ESI and MALDI LC/MS-MS approaches for large scale protein, identification and quantification : Are they equivalent?
(2002) Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics p.55-56- Abstract
An approach for large scale protein identification and quantification was described. The peptide mixtures from the strong cation exchange (SCX) fractionated protein digests were separated and analyzed by HPLC MS/MS. The human fibrobalst nuclei were purified from stimulated and non-stimulated cells. The quantification results with MALDI and ESI LC/MS were shown to be identical within experimental error.
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https://lup.lub.lu.se/record/1c17dd77-3686-4a50-860e-45ee53ab37e5
- author
- organization
- publishing date
- 2002-12-01
- type
- Contribution to conference
- publication status
- published
- subject
- pages
- 2 pages
- conference name
- Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
- conference location
- Orlando, FL, United States
- conference dates
- 2002-06-02 - 2002-06-06
- external identifiers
-
- scopus:2442714022
- language
- English
- LU publication?
- yes
- id
- 1c17dd77-3686-4a50-860e-45ee53ab37e5
- date added to LUP
- 2018-12-10 08:35:31
- date last changed
- 2024-05-13 21:48:29
@misc{1c17dd77-3686-4a50-860e-45ee53ab37e5, abstract = {{<p>An approach for large scale protein identification and quantification was described. The peptide mixtures from the strong cation exchange (SCX) fractionated protein digests were separated and analyzed by HPLC MS/MS. The human fibrobalst nuclei were purified from stimulated and non-stimulated cells. The quantification results with MALDI and ESI LC/MS were shown to be identical within experimental error.</p>}}, author = {{Juhasz, P. and Falick, A. and Graber, A. and Hattan, S. and Khainovski, N. and Marchese, J. and Martin, S. and Patterson, D. and Williamson, B. and Malmstrom, J. and Westergren-Thorsson, G. and Marko-Varga, G.}}, language = {{eng}}, month = {{12}}, pages = {{55--56}}, title = {{ESI and MALDI LC/MS-MS approaches for large scale protein, identification and quantification : Are they equivalent?}}, year = {{2002}}, }