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ESI and MALDI LC/MS-MS approaches for large scale protein, identification and quantification : Are they equivalent?

Juhasz, P. ; Falick, A. ; Graber, A. ; Hattan, S. ; Khainovski, N. ; Marchese, J. ; Martin, S. ; Patterson, D. ; Williamson, B. and Malmstrom, J. LU , et al. (2002) Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics p.55-56
Abstract

An approach for large scale protein identification and quantification was described. The peptide mixtures from the strong cation exchange (SCX) fractionated protein digests were separated and analyzed by HPLC MS/MS. The human fibrobalst nuclei were purified from stimulated and non-stimulated cells. The quantification results with MALDI and ESI LC/MS were shown to be identical within experimental error.

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organization
publishing date
type
Contribution to conference
publication status
published
subject
pages
2 pages
conference name
Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
conference location
Orlando, FL, United States
conference dates
2002-06-02 - 2002-06-06
external identifiers
  • scopus:2442714022
language
English
LU publication?
yes
id
1c17dd77-3686-4a50-860e-45ee53ab37e5
date added to LUP
2018-12-10 08:35:31
date last changed
2021-01-04 14:51:45
@misc{1c17dd77-3686-4a50-860e-45ee53ab37e5,
  abstract     = {<p>An approach for large scale protein identification and quantification was described. The peptide mixtures from the strong cation exchange (SCX) fractionated protein digests were separated and analyzed by HPLC MS/MS. The human fibrobalst nuclei were purified from stimulated and non-stimulated cells. The quantification results with MALDI and ESI LC/MS were shown to be identical within experimental error.</p>},
  author       = {Juhasz, P. and Falick, A. and Graber, A. and Hattan, S. and Khainovski, N. and Marchese, J. and Martin, S. and Patterson, D. and Williamson, B. and Malmstrom, J. and Westergren-Thorsson, G. and Marko-Varga, G.},
  language     = {eng},
  month        = {12},
  pages        = {55--56},
  title        = {ESI and MALDI LC/MS-MS approaches for large scale protein, identification and quantification : Are they equivalent?},
  year         = {2002},
}