The YjbH adaptor protein enhances proteolysis of the transcriptional regulator Spx in Staphylococcus aureus.
(2012) In Journal of Bacteriology 194(5). p.1186-1194- Abstract
- Spx is a global regulator that is widespread among the low G+C Gram-positive bacteria. Spx has been extensively studied in Bacillus subtilis, where it acts as an activator and a repressor of transcription in response to disulfide stress. Under non-stress conditions, Spx is rapidly degraded by the ClpXP protease. This degradation is enhanced by the YjbH adaptor protein. Upon disulfide stress, the amount of Spx rapidly increases due to a decrease in degradation. In the opportunistic pathogen Staphylococcus aureus, Spx is a global regulator influencing growth, biofilm formation and general stress protection, and cells lacking the spx gene exhibit poor growth also under non-stress conditions. To investigate the mechanism by which the activity... (More)
- Spx is a global regulator that is widespread among the low G+C Gram-positive bacteria. Spx has been extensively studied in Bacillus subtilis, where it acts as an activator and a repressor of transcription in response to disulfide stress. Under non-stress conditions, Spx is rapidly degraded by the ClpXP protease. This degradation is enhanced by the YjbH adaptor protein. Upon disulfide stress, the amount of Spx rapidly increases due to a decrease in degradation. In the opportunistic pathogen Staphylococcus aureus, Spx is a global regulator influencing growth, biofilm formation and general stress protection, and cells lacking the spx gene exhibit poor growth also under non-stress conditions. To investigate the mechanism by which the activity of Spx is regulated we identified a homolog in S. aureus of the B. subtilis yjbH gene. The gene encodes a protein that shows approximately 30% sequence identity to YjbH of B. subtilis. Heterologous expression of S. aureus yjbH in a B. subtilis yjbH mutant restored Spx to wild type levels both under non-stress conditions and under conditions of disulfide stress. From these studies we conclude that the two YjbH homologues have a conserved physiological function. Accordingly, inactivation of yjbH in S. aureus increased the level of Spx protein and transcription of the Spx-regulated gene trxB. Notably, the yjbH mutant exhibited reduced growth and increased pigmentation, and both phenotypes were reversed by complementation of the yjbH gene. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2273590
- author
- Engman, Jakob LU ; Rogstam, Annika LU ; Frees, Dorte ; Ingmer, Hanne and von Wachenfeldt, Claes LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Bacteriology
- volume
- 194
- issue
- 5
- pages
- 1186 - 1194
- publisher
- American Society for Microbiology
- external identifiers
-
- wos:000300530800030
- pmid:22194450
- scopus:84857871745
- pmid:22194450
- ISSN
- 0021-9193
- DOI
- 10.1128/JB.06414-11
- language
- English
- LU publication?
- yes
- id
- 1c3eaed5-301d-4c04-a214-ddbb2b169239 (old id 2273590)
- date added to LUP
- 2016-04-01 10:49:23
- date last changed
- 2022-03-20 00:20:19
@article{1c3eaed5-301d-4c04-a214-ddbb2b169239,
abstract = {{Spx is a global regulator that is widespread among the low G+C Gram-positive bacteria. Spx has been extensively studied in Bacillus subtilis, where it acts as an activator and a repressor of transcription in response to disulfide stress. Under non-stress conditions, Spx is rapidly degraded by the ClpXP protease. This degradation is enhanced by the YjbH adaptor protein. Upon disulfide stress, the amount of Spx rapidly increases due to a decrease in degradation. In the opportunistic pathogen Staphylococcus aureus, Spx is a global regulator influencing growth, biofilm formation and general stress protection, and cells lacking the spx gene exhibit poor growth also under non-stress conditions. To investigate the mechanism by which the activity of Spx is regulated we identified a homolog in S. aureus of the B. subtilis yjbH gene. The gene encodes a protein that shows approximately 30% sequence identity to YjbH of B. subtilis. Heterologous expression of S. aureus yjbH in a B. subtilis yjbH mutant restored Spx to wild type levels both under non-stress conditions and under conditions of disulfide stress. From these studies we conclude that the two YjbH homologues have a conserved physiological function. Accordingly, inactivation of yjbH in S. aureus increased the level of Spx protein and transcription of the Spx-regulated gene trxB. Notably, the yjbH mutant exhibited reduced growth and increased pigmentation, and both phenotypes were reversed by complementation of the yjbH gene.}},
author = {{Engman, Jakob and Rogstam, Annika and Frees, Dorte and Ingmer, Hanne and von Wachenfeldt, Claes}},
issn = {{0021-9193}},
language = {{eng}},
number = {{5}},
pages = {{1186--1194}},
publisher = {{American Society for Microbiology}},
series = {{Journal of Bacteriology}},
title = {{The YjbH adaptor protein enhances proteolysis of the transcriptional regulator Spx in Staphylococcus aureus.}},
url = {{http://dx.doi.org/10.1128/JB.06414-11}},
doi = {{10.1128/JB.06414-11}},
volume = {{194}},
year = {{2012}},
}