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Infrared monitoring of the adhesion of Catenaria anguillulae zoospores to solid surfaces

Tunlid, Anders LU ; Nivens, David E. ; Jansson, Hans Börje LU and White, David C. (1991) In Experimental Mycology 15(3). p.206-214
Abstract

Electron microscopic studies of nematodes infected with the chytridiomycetous fungusCatenaria anguillulae indicated that zoospores of the fungus adhered to the cuticle of nematodes by a layer of extracellular polymers. The chemical composition of the adhesive polymers and their interaction with a solid surface were examined with Fourier transform infrared spectroscopy, using an attenuated total reflectance cell. On-line monitoring of the adhesion of zoospores to a germanium crystal with this technique showed that the adhesive polymers consisted of a protein(s) containing amide I and II bands. The adsorption of these proteins, measured as the increase in the amide II band, had a rapid initial phase of ca. 20 minutes, followed by a slower... (More)

Electron microscopic studies of nematodes infected with the chytridiomycetous fungusCatenaria anguillulae indicated that zoospores of the fungus adhered to the cuticle of nematodes by a layer of extracellular polymers. The chemical composition of the adhesive polymers and their interaction with a solid surface were examined with Fourier transform infrared spectroscopy, using an attenuated total reflectance cell. On-line monitoring of the adhesion of zoospores to a germanium crystal with this technique showed that the adhesive polymers consisted of a protein(s) containing amide I and II bands. The adsorption of these proteins, measured as the increase in the amide II band, had a rapid initial phase of ca. 20 minutes, followed by a slower increase during the course of incubation. Fluorescein isothiocyanate staining of the attached cells at the end of the experiment showed that the adhesion of the zoospores occurred before the formation of the cyst wall.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
adhesion, Catenaria anguillulae, FTIR, proteins, zoospores
in
Experimental Mycology
volume
15
issue
3
pages
206 - 214
publisher
Elsevier
external identifiers
  • scopus:0000617757
ISSN
0147-5975
DOI
10.1016/0147-5975(91)90022-6
language
English
LU publication?
yes
id
1c54212b-99aa-4f2f-b27f-6829801afdd1
date added to LUP
2019-10-23 17:23:52
date last changed
2020-01-13 02:28:36
@article{1c54212b-99aa-4f2f-b27f-6829801afdd1,
  abstract     = {<p>Electron microscopic studies of nematodes infected with the chytridiomycetous fungusCatenaria anguillulae indicated that zoospores of the fungus adhered to the cuticle of nematodes by a layer of extracellular polymers. The chemical composition of the adhesive polymers and their interaction with a solid surface were examined with Fourier transform infrared spectroscopy, using an attenuated total reflectance cell. On-line monitoring of the adhesion of zoospores to a germanium crystal with this technique showed that the adhesive polymers consisted of a protein(s) containing amide I and II bands. The adsorption of these proteins, measured as the increase in the amide II band, had a rapid initial phase of ca. 20 minutes, followed by a slower increase during the course of incubation. Fluorescein isothiocyanate staining of the attached cells at the end of the experiment showed that the adhesion of the zoospores occurred before the formation of the cyst wall.</p>},
  author       = {Tunlid, Anders and Nivens, David E. and Jansson, Hans Börje and White, David C.},
  issn         = {0147-5975},
  language     = {eng},
  month        = {01},
  number       = {3},
  pages        = {206--214},
  publisher    = {Elsevier},
  series       = {Experimental Mycology},
  title        = {Infrared monitoring of the adhesion of Catenaria anguillulae zoospores to solid surfaces},
  url          = {http://dx.doi.org/10.1016/0147-5975(91)90022-6},
  doi          = {10.1016/0147-5975(91)90022-6},
  volume       = {15},
  year         = {1991},
}