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A Role for Wnt Signaling in the Reversal of Metabolic Disturbances after Gastric Bypass in Obese Mice

Vangoitsenhoven, Roman ; Van der Schueren, Bart ; da Cunha, Joao Paulo LU ; Lannoo, Matthias ; Mathieu, Chantal ; Overbergh, Lut and Maris, Michael (2015) 75th Scientific Sessions of the American Diabetes Association In Diabetes 64(Suppl 1). p.546-546
Abstract
Gastric bypass surgery has benefi cial effects on obesity-induced metabolic disturbances, but the underlying mechanisms remain unclear. As Wnt
genes are both positively and negatively correlated with obesity-induced
adipose tissue infl ammation and insulin resistance, our main aim is to reveal
a potential role for Wnt signaling in the reversal of these metabolic consequences after gastric bypass surgery in mice.
8-weeks old male C57Bl/6 mice were fed a high fat diet (60 kcal% fat) for
14 weeks, followed by either Roux-en-Y gastric bypass (RYGB; n=13) or Sham
surgery (Sham; n=10). After surgery, mice were maintained on a high fat diet
for another 8 weeks (Sham group was pair fed with the RYGB group). Using
... (More)
Gastric bypass surgery has benefi cial effects on obesity-induced metabolic disturbances, but the underlying mechanisms remain unclear. As Wnt
genes are both positively and negatively correlated with obesity-induced
adipose tissue infl ammation and insulin resistance, our main aim is to reveal
a potential role for Wnt signaling in the reversal of these metabolic consequences after gastric bypass surgery in mice.
8-weeks old male C57Bl/6 mice were fed a high fat diet (60 kcal% fat) for
14 weeks, followed by either Roux-en-Y gastric bypass (RYGB; n=13) or Sham
surgery (Sham; n=10). After surgery, mice were maintained on a high fat diet
for another 8 weeks (Sham group was pair fed with the RYGB group). Using
qRT-PCR, the mRNA expression profi le of Wnt ligands and its antagonists
(i.e. secreted Frizzled-related proteins (sFRP)) was determined in epididymal
white adipose tissue of RYGB and Sham mice.
8 weeks post-surgery, Sham mice returned to their pre-surgery weight
(43.4 g ± 6.9), whereas RYGB mice showed a 43% reduction in body weight,
accompanied by improved metabolic parameters, as compared to Sham mice
(P < 0.01). Epididymal white adipose tissue of RYGB mice showed a 75% and
94% decrease in sFRP4 and sFRP5 mRNA expression levels respectively as
compared to Sham mice (P <0.01). Additionally, a trend towards decreased
mRNA expression of Wnt5a and Wnt5b could be detected in RYGB mice as
compared to Sham mice (P = 0.1).
In conclusion, RYGB surgery strongly reduced the expression level of the
secreted Wnt antagonists sFRP4 and sFRP5 in white adipocyte tissue. As
secreted proteins are interesting drug targets, a potential role for these secreted proteins in the reversal of metabolic disturbances after RYGB surgery
will be studied more in depth.
Supported By: FWOG.0857.13 (Less)
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author
; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
in
Diabetes
volume
64
issue
Suppl 1
pages
546 - 546
publisher
American Diabetes Association Inc.
conference name
75th Scientific Sessions of the American Diabetes Association
conference location
Boston, United States
conference dates
2015-06-05 - 2015-06-09
ISSN
1939-327X
DOI
10.2337/db15-1929-2253
language
English
LU publication?
no
id
1c730618-3d80-423b-b153-d8c3940b8c36
date added to LUP
2020-01-30 16:36:42
date last changed
2020-02-04 13:17:37
@misc{1c730618-3d80-423b-b153-d8c3940b8c36,
  abstract     = {Gastric bypass surgery has benefi cial effects on obesity-induced metabolic disturbances, but the underlying mechanisms remain unclear. As Wnt<br>
genes are both positively and negatively correlated with obesity-induced<br>
adipose tissue infl ammation and insulin resistance, our main aim is to reveal<br>
a potential role for Wnt signaling in the reversal of these metabolic consequences after gastric bypass surgery in mice.<br>
8-weeks old male C57Bl/6 mice were fed a high fat diet (60 kcal% fat) for<br>
14 weeks, followed by either Roux-en-Y gastric bypass (RYGB; n=13) or Sham<br>
surgery (Sham; n=10). After surgery, mice were maintained on a high fat diet<br>
for another 8 weeks (Sham group was pair fed with the RYGB group). Using<br>
qRT-PCR, the mRNA expression profi le of Wnt ligands and its antagonists<br>
(i.e. secreted Frizzled-related proteins (sFRP)) was determined in epididymal<br>
white adipose tissue of RYGB and Sham mice.<br>
8 weeks post-surgery, Sham mice returned to their pre-surgery weight<br>
(43.4 g ± 6.9), whereas RYGB mice showed a 43% reduction in body weight,<br>
accompanied by improved metabolic parameters, as compared to Sham mice<br>
(P &lt; 0.01). Epididymal white adipose tissue of RYGB mice showed a 75% and<br>
94% decrease in sFRP4 and sFRP5 mRNA expression levels respectively as<br>
compared to Sham mice (P &lt;0.01). Additionally, a trend towards decreased<br>
mRNA expression of Wnt5a and Wnt5b could be detected in RYGB mice as<br>
compared to Sham mice (P = 0.1).<br>
In conclusion, RYGB surgery strongly reduced the expression level of the<br>
secreted Wnt antagonists sFRP4 and sFRP5 in white adipocyte tissue. As<br>
secreted proteins are interesting drug targets, a potential role for these secreted proteins in the reversal of metabolic disturbances after RYGB surgery<br>
will be studied more in depth.<br>
Supported By: FWOG.0857.13},
  author       = {Vangoitsenhoven, Roman and Van der Schueren, Bart and da Cunha, Joao Paulo and Lannoo, Matthias and Mathieu, Chantal and Overbergh, Lut and Maris, Michael},
  issn         = {1939-327X},
  language     = {eng},
  note         = {Conference Abstract},
  number       = {Suppl 1},
  pages        = {546--546},
  publisher    = {American Diabetes Association Inc.},
  series       = {Diabetes},
  title        = {A Role for Wnt Signaling in the Reversal of Metabolic Disturbances after Gastric Bypass in Obese Mice},
  url          = {http://dx.doi.org/10.2337/db15-1929-2253},
  doi          = {10.2337/db15-1929-2253},
  volume       = {64},
  year         = {2015},
}