[24] Preparation of Stable Radioiodinated Polypeptide Hormones and Proteins Using Polyacrylamide Gel Electrophoresis
Linde, Susanne ; Hansen, Bruno and Lernmark LU
(1983)
In Methods in Enzymology
92(C).
p.309-335
- Abstract
This chapter discusses the preparation of stable radioiodinated polypeptide hormones and proteins using polyacrylamide gel electrophoresis. Radioactively labeled polypeptide hormones and proteins are widely used as tracers in radioimmunoassays and receptor studies. The peptide or protein is most easily labeled using iodination with 125I or 131I. The radioactive iodine is substituted in the tyrosine groups of the peptide as monoiodotyrosine or di-iodotyrosine, resulting in a heterogeneous mixture of iodine-substituted molecules plus native peptide and unreacted iodide. The iodination mixture has to be fractionated to obtain a well-characterized iodinated product to be used as a tracer. An ideal tracer should retain... (More)
This chapter discusses the preparation of stable radioiodinated polypeptide hormones and proteins using polyacrylamide gel electrophoresis. Radioactively labeled polypeptide hormones and proteins are widely used as tracers in radioimmunoassays and receptor studies. The peptide or protein is most easily labeled using iodination with 125I or 131I. The radioactive iodine is substituted in the tyrosine groups of the peptide as monoiodotyrosine or di-iodotyrosine, resulting in a heterogeneous mixture of iodine-substituted molecules plus native peptide and unreacted iodide. The iodination mixture has to be fractionated to obtain a well-characterized iodinated product to be used as a tracer. An ideal tracer should retain the full biological activity or immunological reactivity of the native peptide hormone or protein and have a high specific activity and a long shelf life. Studies in the laboratory have shown that it is possible to fractionate iodinated insulin preparations into homogeneous monoiodoinsulin derivatives by polyacrylamide gel electrophoresis using long rods. The same fractionation technique has been useful for the preparation of a number of radioiodinated polypeptide hormones and proteins.
(Less)
- author
- Linde, Susanne
; Hansen, Bruno
and Lernmark
LU
- organization
- publishing date
- 1983-01-01
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Methods in Enzymology
- volume
- 92
- issue
- C
- pages
- 27 pages
- publisher
- Academic Press
- external identifiers
-
- scopus:0020690747
- pmid:6343769
- ISSN
- 0076-6879
- DOI
- 10.1016/0076-6879(83)92027-X
- language
- English
- LU publication?
- yes
- id
- 1c748886-1bcc-4fd3-8633-ec891f12a12e
- date added to LUP
- 2019-12-12 23:22:15
- date last changed
- 2024-03-13 08:03:13
@article{1c748886-1bcc-4fd3-8633-ec891f12a12e,
abstract = {{<p>This chapter discusses the preparation of stable radioiodinated polypeptide hormones and proteins using polyacrylamide gel electrophoresis. Radioactively labeled polypeptide hormones and proteins are widely used as tracers in radioimmunoassays and receptor studies. The peptide or protein is most easily labeled using iodination with <sup>125</sup>I or <sup>131</sup>I. The radioactive iodine is substituted in the tyrosine groups of the peptide as monoiodotyrosine or di-iodotyrosine, resulting in a heterogeneous mixture of iodine-substituted molecules plus native peptide and unreacted iodide. The iodination mixture has to be fractionated to obtain a well-characterized iodinated product to be used as a tracer. An ideal tracer should retain the full biological activity or immunological reactivity of the native peptide hormone or protein and have a high specific activity and a long shelf life. Studies in the laboratory have shown that it is possible to fractionate iodinated insulin preparations into homogeneous monoiodoinsulin derivatives by polyacrylamide gel electrophoresis using long rods. The same fractionation technique has been useful for the preparation of a number of radioiodinated polypeptide hormones and proteins.</p>}},
author = {{Linde, Susanne and Hansen, Bruno and Lernmark}},
issn = {{0076-6879}},
language = {{eng}},
month = {{01}},
number = {{C}},
pages = {{309--335}},
publisher = {{Academic Press}},
series = {{Methods in Enzymology}},
title = {{[24] Preparation of Stable Radioiodinated Polypeptide Hormones and Proteins Using Polyacrylamide Gel Electrophoresis}},
url = {{http://dx.doi.org/10.1016/0076-6879(83)92027-X}},
doi = {{10.1016/0076-6879(83)92027-X}},
volume = {{92}},
year = {{1983}},
}