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Molecular characterization of the TUBG1 meshwork’s influence on Cytoskeletal organization

Malycheva, Darina LU and Alvarado Kristensson, Maria LU (2025) In Heliyon 11(2).
Abstract
The γ-tubulin (TUBG) meshwork is a central regulator of cellular architecture, orchestrating processes such as microtubule nucleation, mitochondrial organization, and genomic integrity. This study investigates the molecular impact of TUBG depletion on the cytoskeleton. Knockdown of TUBG using single guide RNA disrupted microtubule, vimentin, and lamin B networks while simultaneously reinforcing actin filaments structures. These findings suggest that actin reinforcement may act as a compensatory response to the broader disruption of cytoskeletal integrity. Expression of N-terminal (TUBG1-335) or C-terminal (TUBG334-451) fragments of TUBG1 partially restored these networks, with the C-terminal fragment demonstrating greater effectiveness... (More)
The γ-tubulin (TUBG) meshwork is a central regulator of cellular architecture, orchestrating processes such as microtubule nucleation, mitochondrial organization, and genomic integrity. This study investigates the molecular impact of TUBG depletion on the cytoskeleton. Knockdown of TUBG using single guide RNA disrupted microtubule, vimentin, and lamin B networks while simultaneously reinforcing actin filaments structures. These findings suggest that actin reinforcement may act as a compensatory response to the broader disruption of cytoskeletal integrity. Expression of N-terminal (TUBG1-335) or C-terminal (TUBG334-451) fragments of TUBG1 partially restored these networks, with the C-terminal fragment demonstrating greater effectiveness reestablishing microtubule integrity. Both fragments stabilized vimentin filaments and the nuclear envelope, underscoring TUBG's dual structural and regulatory roles across multiple cytoskeletal systems. This study highlights the critical hubbing properties of TUBG in coordinating cytoskeletal integrity and its potential as a therapeutic target in cytoskeleton-related disorders. (Less)
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author
and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Heliyon
volume
11
issue
2
article number
e41829
publisher
Elsevier
external identifiers
  • scopus:85215400367
  • pmid:40013266
ISSN
2405-8440
DOI
10.1016/j.heliyon.2025.e41829
language
English
LU publication?
yes
id
1c8e6636-4360-485b-943e-7e5cfa15d23f
date added to LUP
2025-01-15 13:45:14
date last changed
2025-04-17 03:00:07
@article{1c8e6636-4360-485b-943e-7e5cfa15d23f,
  abstract     = {{The γ-tubulin (TUBG) meshwork is a central regulator of cellular architecture, orchestrating processes such as microtubule nucleation, mitochondrial organization, and genomic integrity. This study investigates the molecular impact of TUBG depletion on the cytoskeleton. Knockdown of TUBG using single guide RNA disrupted microtubule, vimentin, and lamin B networks while simultaneously reinforcing actin filaments structures. These findings suggest that actin reinforcement may act as a compensatory response to the broader disruption of cytoskeletal integrity. Expression of N-terminal (TUBG1-335) or C-terminal (TUBG334-451) fragments of TUBG1 partially restored these networks, with the C-terminal fragment demonstrating greater effectiveness reestablishing microtubule integrity. Both fragments stabilized vimentin filaments and the nuclear envelope, underscoring TUBG's dual structural and regulatory roles across multiple cytoskeletal systems. This study highlights the critical hubbing properties of TUBG in coordinating cytoskeletal integrity and its potential as a therapeutic target in cytoskeleton-related disorders.}},
  author       = {{Malycheva, Darina and Alvarado Kristensson, Maria}},
  issn         = {{2405-8440}},
  language     = {{eng}},
  number       = {{2}},
  publisher    = {{Elsevier}},
  series       = {{Heliyon}},
  title        = {{Molecular characterization of the TUBG1 meshwork’s influence on Cytoskeletal organization}},
  url          = {{http://dx.doi.org/10.1016/j.heliyon.2025.e41829}},
  doi          = {{10.1016/j.heliyon.2025.e41829}},
  volume       = {{11}},
  year         = {{2025}},
}