Influence of temperature on water and aqueous glucose absorption spectra in the near- and mid-infrared regions at physiologically relevant temperatures
(2003) In Applied Spectroscopy 57(1). p.28-36- Abstract
- Near- and mid-infrared absorption spectra of pure water and aqueous 1.0 g/dL glucose solutions in the wavenumber range 8000-950 cm(-1) were measured in the temperature range 30-42 C in steps of 2 degreesC. Measurements were carried out with an FT-IR spectrometer and a variable pathlength transmission cell controlled within 0.02 degreesC. Pathlengths of 50 mum and 0.4 mm were used in the mid- and near-infrared spectral region, respectively. Difference spectra were used to determine the effect of temperature on the water spectra quantitatively. These spectra were obtained by subtracting the 37 degreesC water spectrum from the spectra measured at other temperatures. The difference spectra reveal that the effect of temperature is highest in... (More)
- Near- and mid-infrared absorption spectra of pure water and aqueous 1.0 g/dL glucose solutions in the wavenumber range 8000-950 cm(-1) were measured in the temperature range 30-42 C in steps of 2 degreesC. Measurements were carried out with an FT-IR spectrometer and a variable pathlength transmission cell controlled within 0.02 degreesC. Pathlengths of 50 mum and 0.4 mm were used in the mid- and near-infrared spectral region, respectively. Difference spectra were used to determine the effect of temperature on the water spectra quantitatively. These spectra were obtained by subtracting the 37 degreesC water spectrum from the spectra measured at other temperatures. The difference spectra reveal that the effect of temperature is highest in the vicinity of the strong absorption bands, with a number of isosbestic points with no temperature dependence and relatively flat plateaus in between. On the basis of these spectra, prospects for and limitations on data analysis for infrared diagnostic methods are discussed. As an example, the absorptive properties of glucose were studied in the same temperature range in order to determine the effect of temperature on the spectral shape of glucose. The change in water absorption associated with the addition of glucose has also been studied. An estimate of these effects is given and is related to the expected level of infrared signals from glucose in humans. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/318807
- author
- Jensen, PS ; Bak, J and Andersson-Engels, Stefan LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- infrared, principal component analysis, FT-IR, transmission cell, near-infrared, PCA, temperature dependence, water absorption, glucose absorption
- in
- Applied Spectroscopy
- volume
- 57
- issue
- 1
- pages
- 28 - 36
- publisher
- Society for Applied Spectroscopy
- external identifiers
-
- wos:000180902000006
- pmid:14610933
- scopus:0037278155
- ISSN
- 1943-3530
- DOI
- 10.1366/000370203321165179
- language
- English
- LU publication?
- yes
- id
- 1d4c149c-eb88-4e1e-aed9-6fa0f9d3b8ef (old id 318807)
- date added to LUP
- 2016-04-01 12:02:38
- date last changed
- 2022-03-28 19:23:18
@article{1d4c149c-eb88-4e1e-aed9-6fa0f9d3b8ef,
abstract = {{Near- and mid-infrared absorption spectra of pure water and aqueous 1.0 g/dL glucose solutions in the wavenumber range 8000-950 cm(-1) were measured in the temperature range 30-42 C in steps of 2 degreesC. Measurements were carried out with an FT-IR spectrometer and a variable pathlength transmission cell controlled within 0.02 degreesC. Pathlengths of 50 mum and 0.4 mm were used in the mid- and near-infrared spectral region, respectively. Difference spectra were used to determine the effect of temperature on the water spectra quantitatively. These spectra were obtained by subtracting the 37 degreesC water spectrum from the spectra measured at other temperatures. The difference spectra reveal that the effect of temperature is highest in the vicinity of the strong absorption bands, with a number of isosbestic points with no temperature dependence and relatively flat plateaus in between. On the basis of these spectra, prospects for and limitations on data analysis for infrared diagnostic methods are discussed. As an example, the absorptive properties of glucose were studied in the same temperature range in order to determine the effect of temperature on the spectral shape of glucose. The change in water absorption associated with the addition of glucose has also been studied. An estimate of these effects is given and is related to the expected level of infrared signals from glucose in humans.}},
author = {{Jensen, PS and Bak, J and Andersson-Engels, Stefan}},
issn = {{1943-3530}},
keywords = {{infrared; principal component analysis; FT-IR; transmission cell; near-infrared; PCA; temperature dependence; water absorption; glucose absorption}},
language = {{eng}},
number = {{1}},
pages = {{28--36}},
publisher = {{Society for Applied Spectroscopy}},
series = {{Applied Spectroscopy}},
title = {{Influence of temperature on water and aqueous glucose absorption spectra in the near- and mid-infrared regions at physiologically relevant temperatures}},
url = {{https://lup.lub.lu.se/search/files/2756408/2370278.pdf}},
doi = {{10.1366/000370203321165179}},
volume = {{57}},
year = {{2003}},
}