Bet v 1 homologues in strawberry identified as IgE-binding proteins and presumptive allergens
Karlsson, Anne-Li ; Alm, Rikard LU ; Ekstrand, Bo ; Fjelkner-Modig, Stina ; Schiött, Åsa LU ; Bengtsson, Ulf ; Björk, Lars ; Hjerno, Karin ; Roepstorff, Peter and Emanuelsson, Cecilia LU
(2004)
In Allergy
59(12).
p.1277-1284
- Abstract
- Background: No strawberry allergen has so far been identified and characterized.
Methods: Serum samples were collected from patients with a suggestive case history of adverse reactions to strawberry and other fruits. Extracts from fresh
and frozen strawberries were analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), Western blotting and mass spectrometry. Patient blood samples were analysed for inhibition of IgE binding and basophil degranulation.
Results: Several IgE-binding proteins could be detected. In more than half of the patient sera, a 20/18-kDa doublet band was observed in Western blotting. These
two bands were excised and analysed by mass spectrometry showing the... (More) - Background: No strawberry allergen has so far been identified and characterized.
Methods: Serum samples were collected from patients with a suggestive case history of adverse reactions to strawberry and other fruits. Extracts from fresh
and frozen strawberries were analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), Western blotting and mass spectrometry. Patient blood samples were analysed for inhibition of IgE binding and basophil degranulation.
Results: Several IgE-binding proteins could be detected. In more than half of the patient sera, a 20/18-kDa doublet band was observed in Western blotting. These
two bands were excised and analysed by mass spectrometry showing the presence of proteins belonging to the Bet v 1 family of allergens. Inhibition of the IgE binding to the 20/18-kDa doublet was obtained by addition of two recombinantly expressed allergens belonging to the Bet v 1 family (Bet v 1 and Mal d 1) and strawberry protein extract. In a cell-based assay of patient blood samples,
basophil degranulation could be induced by strawberry protein extract and by Bet v 1 and Mal d 1.
Conclusions: We conclude that strawberry homologues to Bet v 1 may be allergens of importance for adverse reactions to strawberry. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/936892
- author
- Karlsson, Anne-Li
; Alm, Rikard
LU
; Ekstrand, Bo
; Fjelkner-Modig, Stina
; Schiött, Åsa
LU
; Bengtsson, Ulf
; Björk, Lars
; Hjerno, Karin
; Roepstorff, Peter
and Emanuelsson, Cecilia
LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- IgE, pollen, food allergy, allergens, clinical immunology
- in
- Allergy
- volume
- 59
- issue
- 12
- pages
- 1277 - 1284
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000224735800006
- scopus:8644243740
- ISSN
- 1398-9995
- DOI
- 10.1111/j.1398-9995.2004.00585.x
- language
- English
- LU publication?
- yes
- id
- 1d8e8a4a-5900-4512-9983-f2c73adf58c0 (old id 936892)
- date added to LUP
- 2016-04-01 15:17:57
- date last changed
- 2022-05-13 10:46:10
@article{1d8e8a4a-5900-4512-9983-f2c73adf58c0,
abstract = {{Background: No strawberry allergen has so far been identified and characterized.<br/><br>
Methods: Serum samples were collected from patients with a suggestive case history of adverse reactions to strawberry and other fruits. Extracts from fresh<br/><br>
and frozen strawberries were analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), Western blotting and mass spectrometry. Patient blood samples were analysed for inhibition of IgE binding and basophil degranulation.<br/><br>
Results: Several IgE-binding proteins could be detected. In more than half of the patient sera, a 20/18-kDa doublet band was observed in Western blotting. These<br/><br>
two bands were excised and analysed by mass spectrometry showing the presence of proteins belonging to the Bet v 1 family of allergens. Inhibition of the IgE binding to the 20/18-kDa doublet was obtained by addition of two recombinantly expressed allergens belonging to the Bet v 1 family (Bet v 1 and Mal d 1) and strawberry protein extract. In a cell-based assay of patient blood samples,<br/><br>
basophil degranulation could be induced by strawberry protein extract and by Bet v 1 and Mal d 1.<br/><br>
Conclusions: We conclude that strawberry homologues to Bet v 1 may be allergens of importance for adverse reactions to strawberry.}},
author = {{Karlsson, Anne-Li and Alm, Rikard and Ekstrand, Bo and Fjelkner-Modig, Stina and Schiött, Åsa and Bengtsson, Ulf and Björk, Lars and Hjerno, Karin and Roepstorff, Peter and Emanuelsson, Cecilia}},
issn = {{1398-9995}},
keywords = {{IgE; pollen; food allergy; allergens; clinical immunology}},
language = {{eng}},
number = {{12}},
pages = {{1277--1284}},
publisher = {{Wiley-Blackwell}},
series = {{Allergy}},
title = {{Bet v 1 homologues in strawberry identified as IgE-binding proteins and presumptive allergens}},
url = {{https://lup.lub.lu.se/search/files/4361773/936941.pdf}},
doi = {{10.1111/j.1398-9995.2004.00585.x}},
volume = {{59}},
year = {{2004}},
}