Essential role of DivIVA in polar growth and morphogenesis in Streptomyces coelicolor A3(2)
(2003) In Molecular Microbiology 49(6). p.1523-1536- Abstract
- Streptomycetes grow by cell wall extension at hyphal
tips. The molecular basis for such polar growth in
prokaryotes is largely unknown. It is reported here
that DivIVA
SC
, the
Streptomyces coelicolor
homologue
of the
Bacillus subtilis
protein DivIVA, is essential
and directly involved in hyphal tip growth and
morphogenesis. A DivIVA
SC
-EGFP hybrid was distinctively
localized to hyphal tips and lateral branches.
Reduction of
divIVA
SC
expression to about 10% of the
normal level produced a phenotype strikingly similar
to that of many tip growth mutants... (More) - Streptomycetes grow by cell wall extension at hyphal
tips. The molecular basis for such polar growth in
prokaryotes is largely unknown. It is reported here
that DivIVA
SC
, the
Streptomyces coelicolor
homologue
of the
Bacillus subtilis
protein DivIVA, is essential
and directly involved in hyphal tip growth and
morphogenesis. A DivIVA
SC
-EGFP hybrid was distinctively
localized to hyphal tips and lateral branches.
Reduction of
divIVA
SC
expression to about 10% of the
normal level produced a phenotype strikingly similar
to that of many tip growth mutants in fungi, including
irregular curly hyphae and apical branching. Overexpression
of the gene dramatically perturbed determination
of cell shape at the growing tips. Furthermore,
staining of nascent peptidoglycan with a fluorescent
vancomycin conjugate revealed that induction of
overexpression in normal hyphae disturbed tip
growth, and gave rise to several new sites of cell wall
assembly, effectively causing hyperbranching. The
results show that DivIVA
SC
is a novel bacterial morphogene,
and it is localized at or very close to the
apical sites of peptidoglycan assembly in
Streptomyces
hyphae. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/770242
- author
- Flärdh, Klas LU
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Molecular Microbiology
- volume
- 49
- issue
- 6
- pages
- 1523 - 1536
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:0141789744
- ISSN
- 1365-2958
- DOI
- 10.1046/j.1365-2958.2003.03660.x
- language
- English
- LU publication?
- no
- id
- 1dd2911c-e59e-4a21-9117-fdcc9ba17984 (old id 770242)
- date added to LUP
- 2016-04-01 12:13:00
- date last changed
- 2022-04-13 07:49:18
@article{1dd2911c-e59e-4a21-9117-fdcc9ba17984,
abstract = {{Streptomycetes grow by cell wall extension at hyphal<br/><br>
tips. The molecular basis for such polar growth in<br/><br>
prokaryotes is largely unknown. It is reported here<br/><br>
that DivIVA<br/><br>
SC<br/><br>
, the<br/><br>
Streptomyces coelicolor<br/><br>
homologue<br/><br>
of the<br/><br>
Bacillus subtilis<br/><br>
protein DivIVA, is essential<br/><br>
and directly involved in hyphal tip growth and<br/><br>
morphogenesis. A DivIVA<br/><br>
SC<br/><br>
-EGFP hybrid was distinctively<br/><br>
localized to hyphal tips and lateral branches.<br/><br>
Reduction of<br/><br>
divIVA<br/><br>
SC<br/><br>
expression to about 10% of the<br/><br>
normal level produced a phenotype strikingly similar<br/><br>
to that of many tip growth mutants in fungi, including<br/><br>
irregular curly hyphae and apical branching. Overexpression<br/><br>
of the gene dramatically perturbed determination<br/><br>
of cell shape at the growing tips. Furthermore,<br/><br>
staining of nascent peptidoglycan with a fluorescent<br/><br>
vancomycin conjugate revealed that induction of<br/><br>
overexpression in normal hyphae disturbed tip<br/><br>
growth, and gave rise to several new sites of cell wall<br/><br>
assembly, effectively causing hyperbranching. The<br/><br>
results show that DivIVA<br/><br>
SC<br/><br>
is a novel bacterial morphogene,<br/><br>
and it is localized at or very close to the<br/><br>
apical sites of peptidoglycan assembly in<br/><br>
Streptomyces<br/><br>
hyphae.}},
author = {{Flärdh, Klas}},
issn = {{1365-2958}},
language = {{eng}},
number = {{6}},
pages = {{1523--1536}},
publisher = {{Wiley-Blackwell}},
series = {{Molecular Microbiology}},
title = {{Essential role of DivIVA in polar growth and morphogenesis in Streptomyces coelicolor A3(2)}},
url = {{http://dx.doi.org/10.1046/j.1365-2958.2003.03660.x}},
doi = {{10.1046/j.1365-2958.2003.03660.x}},
volume = {{49}},
year = {{2003}},
}