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Miniaturized solid-phase extraction and sample preparation for MALDI MS using a microfabricated integrated selective enrichment target

Ekström, Simon LU ; Wallman, Lars LU ; Hok, D; Marko-Varga, György LU and Laurell, Thomas LU (2006) In Journal of Proteome Research 5(5). p.1071-1081
Abstract
A microfabricated proteomic sample preparation and sample presentation device, Integrated Selective Enrichment Target, (ISET), comprising an array of 96 perforated nanovials is described. Each perforated nanovial can be filled with solid-phase extraction media for purification and concentration of peptides prior to matrix-assisted laser desorption/ionization mass spectrometry (MALDI IVIS). The validity of the ISET sample preparation is shown by analysis of low nM-pM standard samples, as well as biological samples. The ISET solid-phase extraction sample preparation was compared to ZipTip and MassPREP PROtarget sample preparation, demonstrating a superior performance with respect to number of detected peptides and signal intensity of... (More)
A microfabricated proteomic sample preparation and sample presentation device, Integrated Selective Enrichment Target, (ISET), comprising an array of 96 perforated nanovials is described. Each perforated nanovial can be filled with solid-phase extraction media for purification and concentration of peptides prior to matrix-assisted laser desorption/ionization mass spectrometry (MALDI IVIS). The validity of the ISET sample preparation is shown by analysis of low nM-pM standard samples, as well as biological samples. The ISET solid-phase extraction sample preparation was compared to ZipTip and MassPREP PROtarget sample preparation, demonstrating a superior performance with respect to number of detected peptides and signal intensity of detected peptides. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
proteomics, spectrometry, mass, SPE, solid-phase extraction, solid-phase microextraction, SPME, integrated selective enrichment target, ISET, MALDI MS
in
Journal of Proteome Research
volume
5
issue
5
pages
1071 - 1081
publisher
The American Chemical Society
external identifiers
  • pmid:16674096
  • wos:000237390200005
  • scopus:33646541053
ISSN
1535-3893
DOI
10.1021/pr050434z
project
CREATE Health
language
English
LU publication?
yes
id
1ddd40ab-ae06-466c-adf2-33114de9daed (old id 410024)
date added to LUP
2007-10-02 14:45:51
date last changed
2019-04-02 01:51:01
@article{1ddd40ab-ae06-466c-adf2-33114de9daed,
  abstract     = {A microfabricated proteomic sample preparation and sample presentation device, Integrated Selective Enrichment Target, (ISET), comprising an array of 96 perforated nanovials is described. Each perforated nanovial can be filled with solid-phase extraction media for purification and concentration of peptides prior to matrix-assisted laser desorption/ionization mass spectrometry (MALDI IVIS). The validity of the ISET sample preparation is shown by analysis of low nM-pM standard samples, as well as biological samples. The ISET solid-phase extraction sample preparation was compared to ZipTip and MassPREP PROtarget sample preparation, demonstrating a superior performance with respect to number of detected peptides and signal intensity of detected peptides.},
  author       = {Ekström, Simon and Wallman, Lars and Hok, D and Marko-Varga, György and Laurell, Thomas},
  issn         = {1535-3893},
  keyword      = {proteomics,spectrometry,mass,SPE,solid-phase extraction,solid-phase microextraction,SPME,integrated selective enrichment target,ISET,MALDI MS},
  language     = {eng},
  number       = {5},
  pages        = {1071--1081},
  publisher    = {The American Chemical Society},
  series       = {Journal of Proteome Research},
  title        = {Miniaturized solid-phase extraction and sample preparation for MALDI MS using a microfabricated integrated selective enrichment target},
  url          = {http://dx.doi.org/10.1021/pr050434z},
  volume       = {5},
  year         = {2006},
}