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Divergence in Coding Sequence and Expression of Different Functional Categories of Immune Genes between Two Wild Rodent Species

Zhong, Xiuqin LU ; Lundberg, Max LU and Råberg, Lars LU (2021) In Genome Biology and Evolution 13(3).
Abstract

Differences in immune function between species could be a result of interspecific divergence in coding sequence and/or expression of immune genes. Here, we investigate how the degree of divergence in coding sequence and expression differs between functional categories of immune genes, and if differences between categories occur independently of other factors (expression level, pleiotropy). To this end, we compared spleen transcriptomes of wild-caught yellow-necked mice and bank voles. Immune genes expressed in the spleen were divided into four categories depending on the function of the encoded protein: pattern recognition receptors (PRR); signal transduction proteins; transcription factors; and cyto- and chemokines and their receptors.... (More)

Differences in immune function between species could be a result of interspecific divergence in coding sequence and/or expression of immune genes. Here, we investigate how the degree of divergence in coding sequence and expression differs between functional categories of immune genes, and if differences between categories occur independently of other factors (expression level, pleiotropy). To this end, we compared spleen transcriptomes of wild-caught yellow-necked mice and bank voles. Immune genes expressed in the spleen were divided into four categories depending on the function of the encoded protein: pattern recognition receptors (PRR); signal transduction proteins; transcription factors; and cyto- and chemokines and their receptors. Genes encoding PRR and cyto-/chemokines had higher sequence divergence than genes encoding signal transduction proteins and transcription factors, even when controlling for potentially confounding factors. Genes encoding PRR also had higher expression divergence than genes encoding signal transduction proteins and transcription factors. There was a positive correlation between expression divergence and coding sequence divergence, in particular for PRR genes. We propose that this is a result of that divergence in PRR coding sequence leads to divergence in PRR expression through positive feedback of PRR ligand binding on PRR expression. When controlling for sequence divergence, expression divergence of PRR genes did not differ from other categories. Taken together, the results indicate that coding sequence divergence of PRR genes is a major cause of differences in immune function between species.

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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Apodemus flavicollis, Myodes glareolus, gene expression evolution, protein evolution, wild immunology
in
Genome Biology and Evolution
volume
13
issue
3
article number
evab023
publisher
Oxford University Press
external identifiers
  • scopus:85102657653
  • pmid:33565592
ISSN
1759-6653
DOI
10.1093/gbe/evab023
language
English
LU publication?
yes
id
1e0cc748-ba4c-4a35-90ba-f286038e3941
date added to LUP
2021-03-24 13:59:08
date last changed
2022-08-04 08:05:16
@article{1e0cc748-ba4c-4a35-90ba-f286038e3941,
  abstract     = {{<p>Differences in immune function between species could be a result of interspecific divergence in coding sequence and/or expression of immune genes. Here, we investigate how the degree of divergence in coding sequence and expression differs between functional categories of immune genes, and if differences between categories occur independently of other factors (expression level, pleiotropy). To this end, we compared spleen transcriptomes of wild-caught yellow-necked mice and bank voles. Immune genes expressed in the spleen were divided into four categories depending on the function of the encoded protein: pattern recognition receptors (PRR); signal transduction proteins; transcription factors; and cyto- and chemokines and their receptors. Genes encoding PRR and cyto-/chemokines had higher sequence divergence than genes encoding signal transduction proteins and transcription factors, even when controlling for potentially confounding factors. Genes encoding PRR also had higher expression divergence than genes encoding signal transduction proteins and transcription factors. There was a positive correlation between expression divergence and coding sequence divergence, in particular for PRR genes. We propose that this is a result of that divergence in PRR coding sequence leads to divergence in PRR expression through positive feedback of PRR ligand binding on PRR expression. When controlling for sequence divergence, expression divergence of PRR genes did not differ from other categories. Taken together, the results indicate that coding sequence divergence of PRR genes is a major cause of differences in immune function between species.</p>}},
  author       = {{Zhong, Xiuqin and Lundberg, Max and Råberg, Lars}},
  issn         = {{1759-6653}},
  keywords     = {{Apodemus flavicollis; Myodes glareolus; gene expression evolution; protein evolution; wild immunology}},
  language     = {{eng}},
  number       = {{3}},
  publisher    = {{Oxford University Press}},
  series       = {{Genome Biology and Evolution}},
  title        = {{Divergence in Coding Sequence and Expression of Different Functional Categories of Immune Genes between Two Wild Rodent Species}},
  url          = {{http://dx.doi.org/10.1093/gbe/evab023}},
  doi          = {{10.1093/gbe/evab023}},
  volume       = {{13}},
  year         = {{2021}},
}