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An mTRAN-mRNA interaction mediates mitochondrial translation initiation in plants

Tran, Huy Cuong LU orcid ; Schmitt, Vivian LU ; Lama, Sbatie LU ; Wang, Chuande ; Launay-Avon, Alexandra ; Bernfur, Katja LU ; Sultan, Kristin LU ; Khan, Kasim LU ; Brunaud, Veronique and Liehrmann, Arnaud , et al. (2023) In Science 381(6661).
Abstract
Plant mitochondria represent the largest group of respiring organelles on the planet. Plant mitochondrial messenger RNAs (mRNAs) lack Shine-Dalgarno-like ribosome-binding sites, so it is unknown how plant mitoribosomes recognize mRNA. We show that “mitochondrial translation factors” mTRAN1 and mTRAN2 are land plant–specific proteins, required for normal mitochondrial respiration chain biogenesis. Our studies suggest that mTRANs are noncanonical pentatricopeptide repeat (PPR)–like RNA binding proteins of the mitoribosomal “small” subunit. We identified conserved Adenosine (A)/Uridine (U)-rich motifs in the 5′ regions of plant mitochondrial mRNAs. mTRAN1 binds this motif, suggesting that it is a mitoribosome homing factor to identify mRNAs.... (More)
Plant mitochondria represent the largest group of respiring organelles on the planet. Plant mitochondrial messenger RNAs (mRNAs) lack Shine-Dalgarno-like ribosome-binding sites, so it is unknown how plant mitoribosomes recognize mRNA. We show that “mitochondrial translation factors” mTRAN1 and mTRAN2 are land plant–specific proteins, required for normal mitochondrial respiration chain biogenesis. Our studies suggest that mTRANs are noncanonical pentatricopeptide repeat (PPR)–like RNA binding proteins of the mitoribosomal “small” subunit. We identified conserved Adenosine (A)/Uridine (U)-rich motifs in the 5′ regions of plant mitochondrial mRNAs. mTRAN1 binds this motif, suggesting that it is a mitoribosome homing factor to identify mRNAs. We demonstrate that mTRANs are likely required for translation of all plant mitochondrial mRNAs. Plant mitochondrial translation initiation thus appears to use a protein-mRNA interaction that is divergent from bacteria or mammalian mitochondria. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Plant, Mitochondria, Ribosome, translation initiation, Arabidopsis
in
Science
volume
381
issue
6661
article number
adg0995
publisher
American Association for the Advancement of Science (AAAS)
external identifiers
  • scopus:85169366277
ISSN
1095-9203
language
English
LU publication?
yes
id
1e34af95-f1e4-4b7c-8a32-84212c54dd16
alternative location
https://www.science.org/doi/10.1126/science.adg0995
date added to LUP
2023-09-13 11:49:06
date last changed
2023-09-20 10:06:10
@article{1e34af95-f1e4-4b7c-8a32-84212c54dd16,
  abstract     = {{Plant mitochondria represent the largest group of respiring organelles on the planet. Plant mitochondrial messenger RNAs (mRNAs) lack Shine-Dalgarno-like ribosome-binding sites, so it is unknown how plant mitoribosomes recognize mRNA. We show that “mitochondrial translation factors” mTRAN1 and mTRAN2 are land plant–specific proteins, required for normal mitochondrial respiration chain biogenesis. Our studies suggest that mTRANs are noncanonical pentatricopeptide repeat (PPR)–like RNA binding proteins of the mitoribosomal “small” subunit. We identified conserved Adenosine (A)/Uridine (U)-rich motifs in the 5′ regions of plant mitochondrial mRNAs. mTRAN1 binds this motif, suggesting that it is a mitoribosome homing factor to identify mRNAs. We demonstrate that mTRANs are likely required for translation of all plant mitochondrial mRNAs. Plant mitochondrial translation initiation thus appears to use a protein-mRNA interaction that is divergent from bacteria or mammalian mitochondria.}},
  author       = {{Tran, Huy Cuong and Schmitt, Vivian and Lama, Sbatie and Wang, Chuande and Launay-Avon, Alexandra and Bernfur, Katja and Sultan, Kristin and Khan, Kasim and Brunaud, Veronique and Liehrmann, Arnaud and Castandet, Benoit and Levander, Fredrik and Rasmusson, Allan and Mireau, Hakim and Delannoy, Etienne and Van Aken, Olivier}},
  issn         = {{1095-9203}},
  keywords     = {{Plant; Mitochondria; Ribosome; translation initiation; Arabidopsis}},
  language     = {{eng}},
  month        = {{09}},
  number       = {{6661}},
  publisher    = {{American Association for the Advancement of Science (AAAS)}},
  series       = {{Science}},
  title        = {{An mTRAN-mRNA interaction mediates mitochondrial translation initiation in plants}},
  url          = {{https://lup.lub.lu.se/search/files/158383236/Tran_et_al_2023_Science_adg0995.pdf}},
  volume       = {{381}},
  year         = {{2023}},
}