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Metabolic Characteristics of Invasive Plant Ipomoea cairica in South China by de novo Transcriptomics

Geng, Yan; Chen, Ling-ling; Lu, Huan; Ning, Chan-juan; Björn, Lars Olof LU and Li, Shao-shan (2016) In Journal of Tropical and Subtropical Botany 24(2). p.128-142
Abstract
To explore the molecular mechanisms of Ipomoea cairica invasiveness insouth China, the de novo transcriptomes from I. cairica and two related species, I. digitata and I. nil,were sequenced and assembled.There were5 6551 all-unigenes obtained by assembling the reads, among them 56522 all-unigenes were annotated, including 7815, 15615, and 180201 all-unigenes in GO, COG and KEGG databases, respectively. Moreover, the activities of NR and GS in I.cairica, keyenzyme in metabolic pathway for nitrogen, were greater than those in related species. In addition, the transcriptome data showed that the genes of key enzymes in secondary metabolism, such aspal, 4cl, cad, chs, and chi, had synergic differential expressionin I. cairica, I. digitata and... (More)
To explore the molecular mechanisms of Ipomoea cairica invasiveness insouth China, the de novo transcriptomes from I. cairica and two related species, I. digitata and I. nil,were sequenced and assembled.There were5 6551 all-unigenes obtained by assembling the reads, among them 56522 all-unigenes were annotated, including 7815, 15615, and 180201 all-unigenes in GO, COG and KEGG databases, respectively. Moreover, the activities of NR and GS in I.cairica, keyenzyme in metabolic pathway for nitrogen, were greater than those in related species. In addition, the transcriptome data showed that the genes of key enzymes in secondary metabolism, such aspal, 4cl, cad, chs, and chi, had synergic differential expressionin I. cairica, I. digitata and I. nil. The production synthesis from metabolic pathway could play a key role in stress-resistant, growth and allelopathy of I. cairica. The RT-qPCR verification results of key genes were similar to those from transcriptome.Therefore, the result of the present research might explain partly the successful invasiveness of I. cairicain South China at the level of molecular biology. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Transcriptome, RNA-seq, Ipomoea cairica, Biological invasion, Metabolism
in
Journal of Tropical and Subtropical Botany
volume
24
issue
2
pages
15 pages
ISSN
1005-3395
DOI
10.11926/j.issn.1005-3395.2016.02.002
language
English
LU publication?
yes
id
1e3ef670-2f3e-4934-8ff8-dcb76fb717aa
date added to LUP
2016-04-11 13:36:51
date last changed
2017-08-10 14:07:23
@article{1e3ef670-2f3e-4934-8ff8-dcb76fb717aa,
  abstract     = {To explore the molecular mechanisms of Ipomoea cairica invasiveness insouth China, the de novo transcriptomes from I. cairica and two related species, I. digitata and I. nil,were sequenced and assembled.There were5 6551 all-unigenes obtained by assembling the reads, among them 56522 all-unigenes were annotated,  including 7815, 15615, and 180201 all-unigenes in GO, COG and KEGG databases, respectively. Moreover, the activities of NR and GS in I.cairica, keyenzyme in metabolic pathway for nitrogen, were greater than those in related species. In addition, the transcriptome data showed that the genes of key enzymes in secondary  metabolism, such aspal, 4cl, cad, chs, and chi, had synergic differential expressionin I. cairica, I. digitata and I. nil. The production synthesis from metabolic pathway could play a key role in stress-resistant, growth and  allelopathy of I. cairica. The RT-qPCR verification results of key genes were similar to those from transcriptome.Therefore, the result of the present research might explain partly the successful invasiveness of I. cairicain South China at the level of molecular biology.},
  author       = {Geng, Yan and Chen, Ling-ling and Lu, Huan and Ning, Chan-juan and Björn, Lars Olof and Li, Shao-shan},
  issn         = {1005-3395},
  keyword      = {Transcriptome,RNA-seq,Ipomoea cairica,Biological invasion,Metabolism},
  language     = {eng},
  number       = {2},
  pages        = {128--142},
  series       = {Journal of Tropical and Subtropical Botany},
  title        = {Metabolic Characteristics of Invasive Plant Ipomoea cairica in South China by de novo Transcriptomics},
  url          = {http://dx.doi.org/10.11926/j.issn.1005-3395.2016.02.002},
  volume       = {24},
  year         = {2016},
}