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Efficient production of HPV16 E2 protein from HPV16 late mRNAs spliced from SD880 to SA2709

Zheng, Yunji LU ; Cui, Xiaoxu LU ; Nilsson, Kersti LU ; Yu, Haoran LU ; Gong, Lijing LU ; Wu, Chengjun LU and Schwartz, Stefan LU (2020) In Virus Research 285.
Abstract

Human papillomaviruses (HPVs) produce a large number of alternatively spliced mRNAs, including a number of differently spliced mRNAs with the potential to produce E2 protein. To identify the alternatively spliced HPV16 mRNA with the highest ability to produce E2 protein, we have generated E2 cDNA expression plasmids representing the most common, alternatively spliced E2 mRNAs, and assessed their translational potential. Our results revealed that an mRNA initiated at the HPV16 late promoter p670 and spliced from the HPV16 5'-splice site SD880 to the HPV16 3'-splice site SA2709, located immediately upstream of the E2 ATG, produced higher levels of E2 than any of the other alternatively spliced, E2-encoding mRNAs. Utilization of a known,... (More)

Human papillomaviruses (HPVs) produce a large number of alternatively spliced mRNAs, including a number of differently spliced mRNAs with the potential to produce E2 protein. To identify the alternatively spliced HPV16 mRNA with the highest ability to produce E2 protein, we have generated E2 cDNA expression plasmids representing the most common, alternatively spliced E2 mRNAs, and assessed their translational potential. Our results revealed that an mRNA initiated at the HPV16 late promoter p670 and spliced from the HPV16 5'-splice site SD880 to the HPV16 3'-splice site SA2709, located immediately upstream of the E2 ATG, produced higher levels of E2 than any of the other alternatively spliced, E2-encoding mRNAs. Utilization of a known, alternative 3'-splice site located upstream of the E2 ATG named SA2582, generated mRNAs with lower ability to produce E2 than mRNAs spliced to SA2709. Finally, analysis of HPV16 mRNA splicing demonstrated that SA2709 was more efficiently spliced to the upstream 5'-splice site SD880 than to the upstream 5'-splice site SD226. In conclusion, the HPV16 mRNA with the greatest ability to produce E2 protein is generated from the HPV16 late promoter and is spliced between HPV16 5'-splice site SD880 and HPV16 3'-splice site SA2709.

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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
E2, HPV16, Splicing, Translation
in
Virus Research
volume
285
article number
198004
publisher
Elsevier
external identifiers
  • scopus:85086524462
  • pmid:32380211
ISSN
1872-7492
DOI
10.1016/j.virusres.2020.198004
language
English
LU publication?
yes
id
1e708618-660f-4dee-94b6-164247503c9e
date added to LUP
2020-07-02 09:01:34
date last changed
2024-06-26 18:19:39
@article{1e708618-660f-4dee-94b6-164247503c9e,
  abstract     = {{<p>Human papillomaviruses (HPVs) produce a large number of alternatively spliced mRNAs, including a number of differently spliced mRNAs with the potential to produce E2 protein. To identify the alternatively spliced HPV16 mRNA with the highest ability to produce E2 protein, we have generated E2 cDNA expression plasmids representing the most common, alternatively spliced E2 mRNAs, and assessed their translational potential. Our results revealed that an mRNA initiated at the HPV16 late promoter p670 and spliced from the HPV16 5'-splice site SD880 to the HPV16 3'-splice site SA2709, located immediately upstream of the E2 ATG, produced higher levels of E2 than any of the other alternatively spliced, E2-encoding mRNAs. Utilization of a known, alternative 3'-splice site located upstream of the E2 ATG named SA2582, generated mRNAs with lower ability to produce E2 than mRNAs spliced to SA2709. Finally, analysis of HPV16 mRNA splicing demonstrated that SA2709 was more efficiently spliced to the upstream 5'-splice site SD880 than to the upstream 5'-splice site SD226. In conclusion, the HPV16 mRNA with the greatest ability to produce E2 protein is generated from the HPV16 late promoter and is spliced between HPV16 5'-splice site SD880 and HPV16 3'-splice site SA2709.</p>}},
  author       = {{Zheng, Yunji and Cui, Xiaoxu and Nilsson, Kersti and Yu, Haoran and Gong, Lijing and Wu, Chengjun and Schwartz, Stefan}},
  issn         = {{1872-7492}},
  keywords     = {{E2; HPV16; Splicing; Translation}},
  language     = {{eng}},
  publisher    = {{Elsevier}},
  series       = {{Virus Research}},
  title        = {{Efficient production of HPV16 E2 protein from HPV16 late mRNAs spliced from SD880 to SA2709}},
  url          = {{http://dx.doi.org/10.1016/j.virusres.2020.198004}},
  doi          = {{10.1016/j.virusres.2020.198004}},
  volume       = {{285}},
  year         = {{2020}},
}