Subventricular Zone-on-a-Chip : A Model to Study Neurogenesis Disruption in Neonatal Intraventricular Hemorrhage
Zamproni, Laura Nicoleti ; Gökçe, Begüm ; Gram, Magnus LU
; Holliday, Coco
; Sendemir, Aylin
; Porcionatto, Marimélia Aparecida
and Herland, Anna
(2025)
In Advanced Science
- Abstract
Intraventricular hemorrhage (IVH) in preterm infants disrupts neurogenesis in the subventricular zone (SVZ), a key neurogenic niche, yet no effective treatments exist. This work develops a human SVZ-on-a-chip model to investigate the inflammatory response in IVH and its impact on neurogenesis. Using this platform, this work examines the effects of red blood cell lysate (RBCL) and hemorrhagic cerebrospinal fluid (CSF) from preterm infants with IVH on SVZ cells. Transcriptomic analysis reveal activation of inflammatory pathways in fetal astrocytes and brain microvascular endothelial cells exposed to hemoglobin isoforms. Notably, interleukin-1B (IL1B) is upregulated following RBCL and hemorrhagic CSF exposure. To probe its role, this work... (More)
Intraventricular hemorrhage (IVH) in preterm infants disrupts neurogenesis in the subventricular zone (SVZ), a key neurogenic niche, yet no effective treatments exist. This work develops a human SVZ-on-a-chip model to investigate the inflammatory response in IVH and its impact on neurogenesis. Using this platform, this work examines the effects of red blood cell lysate (RBCL) and hemorrhagic cerebrospinal fluid (CSF) from preterm infants with IVH on SVZ cells. Transcriptomic analysis reveal activation of inflammatory pathways in fetal astrocytes and brain microvascular endothelial cells exposed to hemoglobin isoforms. Notably, interleukin-1B (IL1B) is upregulated following RBCL and hemorrhagic CSF exposure. To probe its role, this work applies an IL1 receptor antagonist, which demonstrate that IL1B has a partially protective influence on neurogenesis. These findings highlight the SVZ-on-a-chip as a powerful tool for studying IVH pathology and emphasize the role of inflammation in regulating neurogenesis. IL1B emerges as a potential therapeutic target, offering new avenues for intervention. This study advances the understanding of IVH and lays the groundwork for developing strategies to protect the developing brain.
(Less)
- author
- Zamproni, Laura Nicoleti
; Gökçe, Begüm
; Gram, Magnus
LU
; Holliday, Coco
; Sendemir, Aylin
; Porcionatto, Marimélia Aparecida
and Herland, Anna
- organization
- publishing date
- 2025
- type
- Contribution to journal
- publication status
- epub
- subject
- keywords
- cerebrospinal fluid, inflammation, interleukin-1b, intraventricular hemorrhage, neurogenesis, organ-on-a-chip, subventricular zone
- in
- Advanced Science
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- scopus:105019710045
- ISSN
- 2198-3844
- DOI
- 10.1002/advs.202502145
- language
- English
- LU publication?
- yes
- additional info
- Publisher Copyright: © 2025 The Author(s). Advanced Science published by Wiley-VCH GmbH.
- id
- 1ea41aed-989c-4947-898f-3207a421d7c9
- date added to LUP
- 2026-01-16 11:40:21
- date last changed
- 2026-01-16 11:40:33
@article{1ea41aed-989c-4947-898f-3207a421d7c9,
abstract = {{<p>Intraventricular hemorrhage (IVH) in preterm infants disrupts neurogenesis in the subventricular zone (SVZ), a key neurogenic niche, yet no effective treatments exist. This work develops a human SVZ-on-a-chip model to investigate the inflammatory response in IVH and its impact on neurogenesis. Using this platform, this work examines the effects of red blood cell lysate (RBCL) and hemorrhagic cerebrospinal fluid (CSF) from preterm infants with IVH on SVZ cells. Transcriptomic analysis reveal activation of inflammatory pathways in fetal astrocytes and brain microvascular endothelial cells exposed to hemoglobin isoforms. Notably, interleukin-1B (IL1B) is upregulated following RBCL and hemorrhagic CSF exposure. To probe its role, this work applies an IL1 receptor antagonist, which demonstrate that IL1B has a partially protective influence on neurogenesis. These findings highlight the SVZ-on-a-chip as a powerful tool for studying IVH pathology and emphasize the role of inflammation in regulating neurogenesis. IL1B emerges as a potential therapeutic target, offering new avenues for intervention. This study advances the understanding of IVH and lays the groundwork for developing strategies to protect the developing brain.</p>}},
author = {{Zamproni, Laura Nicoleti and Gökçe, Begüm and Gram, Magnus and Holliday, Coco and Sendemir, Aylin and Porcionatto, Marimélia Aparecida and Herland, Anna}},
issn = {{2198-3844}},
keywords = {{cerebrospinal fluid; inflammation; interleukin-1b; intraventricular hemorrhage; neurogenesis; organ-on-a-chip; subventricular zone}},
language = {{eng}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Advanced Science}},
title = {{Subventricular Zone-on-a-Chip : A Model to Study Neurogenesis Disruption in Neonatal Intraventricular Hemorrhage}},
url = {{http://dx.doi.org/10.1002/advs.202502145}},
doi = {{10.1002/advs.202502145}},
year = {{2025}},
}