Spontaneous Formation of Cushioned Model Membranes Promoted by an Intrinsically Disordered Protein
(2020) In Langmuir 36(15). p.3997-4004- Abstract
In this article, it is shown that by exposing commonly used lipids for biomembrane mimicking studies, to a solution containing the histidine-rich intrinsically disordered protein histatin 5, a protein cushion spontaneously forms underneath the bilayer. The underlying mechanism is attributed to have an electrostatic origin, and it is hypothesized that the observed behavior is due to proton charge fluctuations promoting attractive electrostatic interactions between the positively charged proteins and the anionic surfaces, with concomitant counterion release. Hence, we anticipate that this novel "green" approach of forming cushioned bilayers can be an important tool to mimic the cell membrane without the disturbance of the solid substrate,... (More)
In this article, it is shown that by exposing commonly used lipids for biomembrane mimicking studies, to a solution containing the histidine-rich intrinsically disordered protein histatin 5, a protein cushion spontaneously forms underneath the bilayer. The underlying mechanism is attributed to have an electrostatic origin, and it is hypothesized that the observed behavior is due to proton charge fluctuations promoting attractive electrostatic interactions between the positively charged proteins and the anionic surfaces, with concomitant counterion release. Hence, we anticipate that this novel "green" approach of forming cushioned bilayers can be an important tool to mimic the cell membrane without the disturbance of the solid substrate, thereby achieving a further understanding of protein-cell interactions.
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- author
- Gerelli, Yuri ; Eriksson Skog, Amanda LU ; Jephthah, Stephanie LU ; Welbourn, Rebecca J.L. ; Klechikov, Alexey and Skepö, Marie LU
- organization
- publishing date
- 2020
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Langmuir
- volume
- 36
- issue
- 15
- pages
- 8 pages
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- scopus:85083912949
- pmid:32212610
- ISSN
- 0743-7463
- DOI
- 10.1021/acs.langmuir.0c00120
- language
- English
- LU publication?
- yes
- id
- 1ed72331-f8b9-4234-aeb0-c5ab36907584
- date added to LUP
- 2020-05-20 09:27:18
- date last changed
- 2024-06-12 14:29:11
@article{1ed72331-f8b9-4234-aeb0-c5ab36907584,
abstract = {{<p>In this article, it is shown that by exposing commonly used lipids for biomembrane mimicking studies, to a solution containing the histidine-rich intrinsically disordered protein histatin 5, a protein cushion spontaneously forms underneath the bilayer. The underlying mechanism is attributed to have an electrostatic origin, and it is hypothesized that the observed behavior is due to proton charge fluctuations promoting attractive electrostatic interactions between the positively charged proteins and the anionic surfaces, with concomitant counterion release. Hence, we anticipate that this novel "green" approach of forming cushioned bilayers can be an important tool to mimic the cell membrane without the disturbance of the solid substrate, thereby achieving a further understanding of protein-cell interactions.</p>}},
author = {{Gerelli, Yuri and Eriksson Skog, Amanda and Jephthah, Stephanie and Welbourn, Rebecca J.L. and Klechikov, Alexey and Skepö, Marie}},
issn = {{0743-7463}},
language = {{eng}},
number = {{15}},
pages = {{3997--4004}},
publisher = {{The American Chemical Society (ACS)}},
series = {{Langmuir}},
title = {{Spontaneous Formation of Cushioned Model Membranes Promoted by an Intrinsically Disordered Protein}},
url = {{http://dx.doi.org/10.1021/acs.langmuir.0c00120}},
doi = {{10.1021/acs.langmuir.0c00120}},
volume = {{36}},
year = {{2020}},
}