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Adenosine causes read-through into the late region of the HPV16 genome in a guanosine-dependent manner

Yu, Haoran LU ; Wu, Chengjun ; Nilsson, Kersti LU ; Kajitani, Naoko LU and Schwartz, Stefan LU (2018) In Virology 521. p.1-19
Abstract

Adenosine plays an important role in cell death and differentiation as well as in tumorigenesis and the intra- and extra-cellular levels range from nanomolar to millimolar levels under various physiological or pathophysiological conditions. Here we report that adenosine can activate HPV16 late gene expression in a dose- and time-dependent manner, but only in the presence of guanosine. This activation occurred within hours after addition of the nucleosides and was primarily dependent on the ENT1 nucleoside transporter protein. Induction of HPV16 late gene expression was mainly the result of increased read-through at the early HPV16 polyadenylation signal into the late region of the HPV16 genome, thereby producing HPV16 late L2 mRNAs. The... (More)

Adenosine plays an important role in cell death and differentiation as well as in tumorigenesis and the intra- and extra-cellular levels range from nanomolar to millimolar levels under various physiological or pathophysiological conditions. Here we report that adenosine can activate HPV16 late gene expression in a dose- and time-dependent manner, but only in the presence of guanosine. This activation occurred within hours after addition of the nucleosides and was primarily dependent on the ENT1 nucleoside transporter protein. Induction of HPV16 late gene expression was mainly the result of increased read-through at the early HPV16 polyadenylation signal into the late region of the HPV16 genome, thereby producing HPV16 late L2 mRNAs. The effect of guanosine and adenosine on HPV16 late gene expression was mediated by the increased binding to HPV16 mRNAs and nuclear export of the cellular HuR protein. Our results demonstrate that nucleosides can affect HPV16 gene expression.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Adenosine, ENT1, HPV16, HuR, Nucleoside, Papillomavirus, Polyadenylation, Purine, Splicing
in
Virology
volume
521
pages
19 pages
publisher
Elsevier
external identifiers
  • pmid:29864673
  • scopus:85048497658
ISSN
0042-6822
DOI
10.1016/j.virol.2018.05.019
language
English
LU publication?
yes
id
1fffba94-dbbd-414b-baec-8789340646a4
date added to LUP
2018-06-28 11:26:51
date last changed
2020-01-13 00:47:56
@article{1fffba94-dbbd-414b-baec-8789340646a4,
  abstract     = {<p>Adenosine plays an important role in cell death and differentiation as well as in tumorigenesis and the intra- and extra-cellular levels range from nanomolar to millimolar levels under various physiological or pathophysiological conditions. Here we report that adenosine can activate HPV16 late gene expression in a dose- and time-dependent manner, but only in the presence of guanosine. This activation occurred within hours after addition of the nucleosides and was primarily dependent on the ENT1 nucleoside transporter protein. Induction of HPV16 late gene expression was mainly the result of increased read-through at the early HPV16 polyadenylation signal into the late region of the HPV16 genome, thereby producing HPV16 late L2 mRNAs. The effect of guanosine and adenosine on HPV16 late gene expression was mediated by the increased binding to HPV16 mRNAs and nuclear export of the cellular HuR protein. Our results demonstrate that nucleosides can affect HPV16 gene expression.</p>},
  author       = {Yu, Haoran and Wu, Chengjun and Nilsson, Kersti and Kajitani, Naoko and Schwartz, Stefan},
  issn         = {0042-6822},
  language     = {eng},
  month        = {08},
  pages        = {1--19},
  publisher    = {Elsevier},
  series       = {Virology},
  title        = {Adenosine causes read-through into the late region of the HPV16 genome in a guanosine-dependent manner},
  url          = {http://dx.doi.org/10.1016/j.virol.2018.05.019},
  doi          = {10.1016/j.virol.2018.05.019},
  volume       = {521},
  year         = {2018},
}