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MRM assay for quantitation of complement components in human blood plasma - a feasibility study on multiple sclerosis.

Rezeli, Melinda LU ; Végvári, Ákos LU ; Ottervald, Jan; Olsson, Tomas LU ; Laurell, Thomas LU and Marko-Varga, György LU (2011) In Journal of Proteomics 75(1). p.211-220
Abstract
As a proof-of-principle study, a multiple reaction monitoring (MRM) assay was developed for quantitation of proteotypic peptides, representing seven plasma proteins associated with inflammation (complement components and C-reactive protein). The assay development and the sample analysis were performed on a linear ion trap mass spectrometer. We were able to quantify 5 of the 7 target proteins in depleted plasma digests with reasonable reproducibility over a 2 orders of magnitude linear range (RSD < 25%). The assay panel was utilized for the analysis of a small multiple sclerosis sample cohort with 10 diseased and 8 control patients.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
linear ion trap, multiple sclerosis, selective reaction monitoring
in
Journal of Proteomics
volume
75
issue
1
pages
211 - 220
publisher
Elsevier
external identifiers
  • wos:000298710400021
  • pmid:21703369
  • scopus:82955196277
ISSN
1874-3919
DOI
10.1016/j.jprot.2011.05.042
language
English
LU publication?
yes
id
a263c94a-0257-4f07-9959-2cb4c36b2486 (old id 2007751)
date added to LUP
2011-07-08 09:15:55
date last changed
2017-09-24 03:12:28
@article{a263c94a-0257-4f07-9959-2cb4c36b2486,
  abstract     = {As a proof-of-principle study, a multiple reaction monitoring (MRM) assay was developed for quantitation of proteotypic peptides, representing seven plasma proteins associated with inflammation (complement components and C-reactive protein). The assay development and the sample analysis were performed on a linear ion trap mass spectrometer. We were able to quantify 5 of the 7 target proteins in depleted plasma digests with reasonable reproducibility over a 2 orders of magnitude linear range (RSD &lt; 25%). The assay panel was utilized for the analysis of a small multiple sclerosis sample cohort with 10 diseased and 8 control patients.},
  author       = {Rezeli, Melinda and Végvári, Ákos and Ottervald, Jan and Olsson, Tomas and Laurell, Thomas and Marko-Varga, György},
  issn         = {1874-3919},
  keyword      = {linear ion trap,multiple sclerosis,selective reaction monitoring},
  language     = {eng},
  number       = {1},
  pages        = {211--220},
  publisher    = {Elsevier},
  series       = {Journal of Proteomics},
  title        = {MRM assay for quantitation of complement components in human blood plasma - a feasibility study on multiple sclerosis.},
  url          = {http://dx.doi.org/10.1016/j.jprot.2011.05.042},
  volume       = {75},
  year         = {2011},
}