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MRM assay for quantitation of complement components in human blood plasma - a feasibility study on multiple sclerosis.

Rezeli, Melinda LU orcid ; Végvári, Ákos LU ; Ottervald, Jan ; Olsson, Tomas LU ; Laurell, Thomas LU and Marko-Varga, György LU (2011) In Journal of Proteomics 75(1). p.211-220
Abstract
As a proof-of-principle study, a multiple reaction monitoring (MRM) assay was developed for quantitation of proteotypic peptides, representing seven plasma proteins associated with inflammation (complement components and C-reactive protein). The assay development and the sample analysis were performed on a linear ion trap mass spectrometer. We were able to quantify 5 of the 7 target proteins in depleted plasma digests with reasonable reproducibility over a 2 orders of magnitude linear range (RSD < 25%). The assay panel was utilized for the analysis of a small multiple sclerosis sample cohort with 10 diseased and 8 control patients.
Please use this url to cite or link to this publication:
author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
linear ion trap, multiple sclerosis, selective reaction monitoring
in
Journal of Proteomics
volume
75
issue
1
pages
211 - 220
publisher
Elsevier
external identifiers
  • wos:000298710400021
  • pmid:21703369
  • scopus:82955196277
  • pmid:21703369
ISSN
1874-3919
DOI
10.1016/j.jprot.2011.05.042
language
English
LU publication?
yes
id
a263c94a-0257-4f07-9959-2cb4c36b2486 (old id 2007751)
date added to LUP
2016-04-01 10:26:34
date last changed
2025-04-04 13:55:22
@article{a263c94a-0257-4f07-9959-2cb4c36b2486,
  abstract     = {{As a proof-of-principle study, a multiple reaction monitoring (MRM) assay was developed for quantitation of proteotypic peptides, representing seven plasma proteins associated with inflammation (complement components and C-reactive protein). The assay development and the sample analysis were performed on a linear ion trap mass spectrometer. We were able to quantify 5 of the 7 target proteins in depleted plasma digests with reasonable reproducibility over a 2 orders of magnitude linear range (RSD &lt; 25%). The assay panel was utilized for the analysis of a small multiple sclerosis sample cohort with 10 diseased and 8 control patients.}},
  author       = {{Rezeli, Melinda and Végvári, Ákos and Ottervald, Jan and Olsson, Tomas and Laurell, Thomas and Marko-Varga, György}},
  issn         = {{1874-3919}},
  keywords     = {{linear ion trap; multiple sclerosis; selective reaction monitoring}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{211--220}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Proteomics}},
  title        = {{MRM assay for quantitation of complement components in human blood plasma - a feasibility study on multiple sclerosis.}},
  url          = {{https://lup.lub.lu.se/search/files/1848029/2028880.pdf}},
  doi          = {{10.1016/j.jprot.2011.05.042}},
  volume       = {{75}},
  year         = {{2011}},
}