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Regeneration in, and properties of, extracted peripheral nerve allografts and xenografts.

Kvist, Martin LU ; Sondell, Mariann; Kanje, Martin and Dahlin, Lars LU (2011) In Journal of Plastic Surgery and Hand Surgery 45(3). p.122-128
Abstract
Abstract When not enough conventional autologous nerve grafts are available, alternatives are needed to bridge nerve defects. Our aim was to study regeneration of nerves in chemically-extracted acellular nerve grafts from frogs, mice, humans (fresh and stored sural nerve), pigs and rats when defects in rat sciatic nerves were bridged. Secondly, we compared two different extraction procedures (techniques described by Sondell et al. and Hudson et al.) with respect to how efficiently they supported axonal outgrowth, and remaining laminin and myelin basic protein (MBP), after extraction. Isografts (rat) and xenografts (mouse) were transplanted into defects in rat sciatic nerves. Acellular nerve allografts from rats, extracted by the Sondell et... (More)
Abstract When not enough conventional autologous nerve grafts are available, alternatives are needed to bridge nerve defects. Our aim was to study regeneration of nerves in chemically-extracted acellular nerve grafts from frogs, mice, humans (fresh and stored sural nerve), pigs and rats when defects in rat sciatic nerves were bridged. Secondly, we compared two different extraction procedures (techniques described by Sondell et al. and Hudson et al.) with respect to how efficiently they supported axonal outgrowth, and remaining laminin and myelin basic protein (MBP), after extraction. Isografts (rat) and xenografts (mouse) were transplanted into defects in rat sciatic nerves. Acellular nerve allografts from rats, extracted by the Sondell et al's technique, had an appreciably longer axonal outgrowth based on immunohistochemical staining of neurofilaments, than acellular nerve xenografts except those from the pig. Among acellular xenografts there was considerably longer axonal outgrowth in the grafts from pigs compared with those from humans (fresh), but there were no other differences among the xenografts with respect to axonal outgrowth. Axonal outgrowth in acellular nerve xenografts from mice, extracted by the method described by Sondell et al. was longer than in those extracted by Hudson et al's method, while there was no difference in outgrowth between extracted nerve isografts from rats. Electrophoretic analysis of extracted acellular nerve grafts showed remaining laminin, but not MBP, after both extraction procedures. These preserved laminin and removed MBP in acellular nerve grafts. Such grafts can be used to reconstruct short defects in nerves irrespective of their origin. However, selecting and matching a suitable combination of graft and host species may improve axonal outgrowth. (Less)
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type
Contribution to journal
publication status
published
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in
Journal of Plastic Surgery and Hand Surgery
volume
45
issue
3
pages
122 - 128
publisher
Taylor & Francis
external identifiers
  • wos:000291815000001
  • pmid:21682608
  • scopus:79959407377
ISSN
2000-656X
DOI
10.3109/2000656X.2011.571847
language
English
LU publication?
yes
id
52c185e3-ac79-427b-bb5a-f1c102145df4 (old id 2007946)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21682608?dopt=Abstract
date added to LUP
2011-07-06 10:07:09
date last changed
2017-10-01 03:28:09
@article{52c185e3-ac79-427b-bb5a-f1c102145df4,
  abstract     = {Abstract When not enough conventional autologous nerve grafts are available, alternatives are needed to bridge nerve defects. Our aim was to study regeneration of nerves in chemically-extracted acellular nerve grafts from frogs, mice, humans (fresh and stored sural nerve), pigs and rats when defects in rat sciatic nerves were bridged. Secondly, we compared two different extraction procedures (techniques described by Sondell et al. and Hudson et al.) with respect to how efficiently they supported axonal outgrowth, and remaining laminin and myelin basic protein (MBP), after extraction. Isografts (rat) and xenografts (mouse) were transplanted into defects in rat sciatic nerves. Acellular nerve allografts from rats, extracted by the Sondell et al's technique, had an appreciably longer axonal outgrowth based on immunohistochemical staining of neurofilaments, than acellular nerve xenografts except those from the pig. Among acellular xenografts there was considerably longer axonal outgrowth in the grafts from pigs compared with those from humans (fresh), but there were no other differences among the xenografts with respect to axonal outgrowth. Axonal outgrowth in acellular nerve xenografts from mice, extracted by the method described by Sondell et al. was longer than in those extracted by Hudson et al's method, while there was no difference in outgrowth between extracted nerve isografts from rats. Electrophoretic analysis of extracted acellular nerve grafts showed remaining laminin, but not MBP, after both extraction procedures. These preserved laminin and removed MBP in acellular nerve grafts. Such grafts can be used to reconstruct short defects in nerves irrespective of their origin. However, selecting and matching a suitable combination of graft and host species may improve axonal outgrowth.},
  author       = {Kvist, Martin and Sondell, Mariann and Kanje, Martin and Dahlin, Lars},
  issn         = {2000-656X},
  language     = {eng},
  number       = {3},
  pages        = {122--128},
  publisher    = {Taylor & Francis},
  series       = {Journal of Plastic Surgery and Hand Surgery},
  title        = {Regeneration in, and properties of, extracted peripheral nerve allografts and xenografts.},
  url          = {http://dx.doi.org/10.3109/2000656X.2011.571847},
  volume       = {45},
  year         = {2011},
}