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The neuroprotective effect of 2-oxoglutarate in the experimental ischemia of hippocampus

Kovalenko, T. N.; Ushakova, G. A.; Osadchenko, I.; Skibo, G. G. and Pierzynowski, Stefan LU (2011) In Journal of Physiology and Pharmacology 62(2). p.239-246
Abstract
In this study we investigated the potential neuroprotective effect of 2-oxoglutarate (2-OG) on the hippocampus in the transient vessel occlusion ischemia model in the Mongolian gerbil. The morphological and biochemical studies were performed at 7 days after occlusion of carotid arteries. The acute reduction of NeuN-positive neurons in the CA1 pyramidal layer of the hippocampus was accompanied by increased staining intensity for GFAP-positive astrocytes, indicative of glial reaction. The neuron death in the CA1 area coincided with a strong 2.4 fold decrease in the membrane forms of neuronal cell adhesion molecules and elevated levels of astrocyte-specific proteins (soluble GFAP to 2,6 times; filament GFAP to 1,5 times; calcium-binding... (More)
In this study we investigated the potential neuroprotective effect of 2-oxoglutarate (2-OG) on the hippocampus in the transient vessel occlusion ischemia model in the Mongolian gerbil. The morphological and biochemical studies were performed at 7 days after occlusion of carotid arteries. The acute reduction of NeuN-positive neurons in the CA1 pyramidal layer of the hippocampus was accompanied by increased staining intensity for GFAP-positive astrocytes, indicative of glial reaction. The neuron death in the CA1 area coincided with a strong 2.4 fold decrease in the membrane forms of neuronal cell adhesion molecules and elevated levels of astrocyte-specific proteins (soluble GFAP to 2,6 times; filament GFAP to 1,5 times; calcium-binding protein S-100b to 1,6 times). Treatment with 2-oxoglutarate (2.28 g/l drinking water) for between 7 and 21 days attenuated the neuronal death and reactive astrogliosis in this model of experimental ischemia by 20-50%. Our results suggest that 2-OG may prevent the disturbances of neural cells that usually take place during ischemic pathology. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
2-oxoglutarate, brain, glial fibrillary acid protein (GFAP), hippocampus, ischemia, neural cell adhesion molecule (NCAM), neuronal, specific nuclear protein (NeuN), S100 calcium binding protein B (S100b)
in
Journal of Physiology and Pharmacology
volume
62
issue
2
pages
239 - 246
publisher
Polish Physiological Society
external identifiers
  • wos:000291769100012
  • scopus:79959342918
ISSN
0867-5910
language
English
LU publication?
yes
id
24d4838c-3069-4e22-8635-857234d648e5 (old id 2052554)
alternative location
http://www.jpp.krakow.pl/journal/archive/04_11/pdf/239_04_11_article.pdf
date added to LUP
2011-07-26 12:26:06
date last changed
2017-09-17 06:50:34
@article{24d4838c-3069-4e22-8635-857234d648e5,
  abstract     = {In this study we investigated the potential neuroprotective effect of 2-oxoglutarate (2-OG) on the hippocampus in the transient vessel occlusion ischemia model in the Mongolian gerbil. The morphological and biochemical studies were performed at 7 days after occlusion of carotid arteries. The acute reduction of NeuN-positive neurons in the CA1 pyramidal layer of the hippocampus was accompanied by increased staining intensity for GFAP-positive astrocytes, indicative of glial reaction. The neuron death in the CA1 area coincided with a strong 2.4 fold decrease in the membrane forms of neuronal cell adhesion molecules and elevated levels of astrocyte-specific proteins (soluble GFAP to 2,6 times; filament GFAP to 1,5 times; calcium-binding protein S-100b to 1,6 times). Treatment with 2-oxoglutarate (2.28 g/l drinking water) for between 7 and 21 days attenuated the neuronal death and reactive astrogliosis in this model of experimental ischemia by 20-50%. Our results suggest that 2-OG may prevent the disturbances of neural cells that usually take place during ischemic pathology.},
  author       = {Kovalenko, T. N. and Ushakova, G. A. and Osadchenko, I. and Skibo, G. G. and Pierzynowski, Stefan},
  issn         = {0867-5910},
  keyword      = {2-oxoglutarate,brain,glial fibrillary acid protein (GFAP),hippocampus,ischemia,neural cell adhesion molecule (NCAM),neuronal,specific nuclear protein (NeuN),S100 calcium binding protein B (S100b)},
  language     = {eng},
  number       = {2},
  pages        = {239--246},
  publisher    = {Polish Physiological Society},
  series       = {Journal of Physiology and Pharmacology},
  title        = {The neuroprotective effect of 2-oxoglutarate in the experimental ischemia of hippocampus},
  volume       = {62},
  year         = {2011},
}