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Drug quantification in turbid media by fluorescence imaging combined with light-absorption correction using white Monte Carlo simulations.

Xie, Haiyan LU ; Liu, Haichun LU ; Svenmarker, Pontus LU ; Axelsson, Johan LU ; Xu, Can LU ; Gräfe, Susanna; Holm, Jesper LU ; Cheng, Haynes Pak Hay; Svanberg, Sune LU and Bendsöe, Niels LU , et al. (2011) In Journal of Biomedical Optics 16(6).
Abstract
Accurate quantification of photosensitizers is in many cases a critical issue in photodynamic therapy. As a noninvasive and sensitive tool, fluorescence imaging has attracted particular interest for quantification in pre-clinical research. However, due to the absorption of excitation and emission light by turbid media, such as biological tissue, the detected fluorescence signal does not have a simple and unique dependence on the fluorophore concentration for different tissues, but depends in a complex way on other parameters as well. For this reason, little has been done on drug quantification in vivo by the fluorescence imaging technique. In this paper we present a novel approach to compensate for the light absorption in homogeneous... (More)
Accurate quantification of photosensitizers is in many cases a critical issue in photodynamic therapy. As a noninvasive and sensitive tool, fluorescence imaging has attracted particular interest for quantification in pre-clinical research. However, due to the absorption of excitation and emission light by turbid media, such as biological tissue, the detected fluorescence signal does not have a simple and unique dependence on the fluorophore concentration for different tissues, but depends in a complex way on other parameters as well. For this reason, little has been done on drug quantification in vivo by the fluorescence imaging technique. In this paper we present a novel approach to compensate for the light absorption in homogeneous turbid media both for the excitation and emission light, utilizing time-resolved fluorescence white Monte Carlo simulations combined with the Beer-Lambert law. This method shows that the corrected fluorescence intensity is almost proportional to the absolute fluorophore concentration. The results on controllable tissue phantoms and murine tissues are presented and show good correlations between the evaluated fluorescence intensities after the light-absorption correction and absolute fluorophore concentrations. These results suggest that the technique potentially provides the means to quantify the fluorophore concentration from fluorescence images. (Less)
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published
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Journal of Biomedical Optics
volume
16
issue
6
publisher
Published by SPIE--the International Society for Optical Engineering in cooperation with International Biomedical Optics Society
external identifiers
  • wos:000293086800008
  • pmid:21721803
  • scopus:79958108697
ISSN
1083-3668
DOI
10.1117/1.3585675
language
English
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yes
id
84697669-5d10-41b9-b4bf-07e7b765d97f (old id 2059154)
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http://www.ncbi.nlm.nih.gov/pubmed/21721803?dopt=Abstract
date added to LUP
2011-08-01 18:29:10
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2017-01-01 04:06:58
@article{84697669-5d10-41b9-b4bf-07e7b765d97f,
  abstract     = {Accurate quantification of photosensitizers is in many cases a critical issue in photodynamic therapy. As a noninvasive and sensitive tool, fluorescence imaging has attracted particular interest for quantification in pre-clinical research. However, due to the absorption of excitation and emission light by turbid media, such as biological tissue, the detected fluorescence signal does not have a simple and unique dependence on the fluorophore concentration for different tissues, but depends in a complex way on other parameters as well. For this reason, little has been done on drug quantification in vivo by the fluorescence imaging technique. In this paper we present a novel approach to compensate for the light absorption in homogeneous turbid media both for the excitation and emission light, utilizing time-resolved fluorescence white Monte Carlo simulations combined with the Beer-Lambert law. This method shows that the corrected fluorescence intensity is almost proportional to the absolute fluorophore concentration. The results on controllable tissue phantoms and murine tissues are presented and show good correlations between the evaluated fluorescence intensities after the light-absorption correction and absolute fluorophore concentrations. These results suggest that the technique potentially provides the means to quantify the fluorophore concentration from fluorescence images.},
  articleno    = {066002},
  author       = {Xie, Haiyan and Liu, Haichun and Svenmarker, Pontus and Axelsson, Johan and Xu, Can and Gräfe, Susanna and Holm, Jesper and Cheng, Haynes Pak Hay and Svanberg, Sune and Bendsöe, Niels and Andersen, Peter and Svanberg, Katarina and Andersson-Engels, Stefan},
  issn         = {1083-3668},
  language     = {eng},
  number       = {6},
  publisher    = {Published by SPIE--the International Society for Optical Engineering in cooperation with International Biomedical Optics Society},
  series       = {Journal of Biomedical Optics},
  title        = {Drug quantification in turbid media by fluorescence imaging combined with light-absorption correction using white Monte Carlo simulations.},
  url          = {http://dx.doi.org/10.1117/1.3585675},
  volume       = {16},
  year         = {2011},
}