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High-capacity composite adsorbents for nucleic acids.

Tiainen, Peter LU ; Rokebul Anower, M and Larsson, Per-Olof LU (2011) In Journal of chromatography. A 1218(31). p.5235-5240
Abstract
Cytopore is a bead-shaped, macroporous and easily compressible cellulose-based anion-exchange material intended for cultivation of anchor-dependent animal cells. Reticulated vitreous carbon (RVC) is a strong, non-compressible, high voidage (97%) matrix material that can be cut to desired geometrical shapes. Cytopore and RVC were combined to cylindrical composites (25mm 10mm) fitted inside chromatography columns. The composite combined the advantageous properties of both its constituents, making it suitable for column chromatography. The composite could withstand very high flow rates without compaction of the bed (>25column volumes/min; 4000cmh(-1)). Chromatography runs with tracers showed a low HETP value (0.3mm), suggesting that pore... (More)
Cytopore is a bead-shaped, macroporous and easily compressible cellulose-based anion-exchange material intended for cultivation of anchor-dependent animal cells. Reticulated vitreous carbon (RVC) is a strong, non-compressible, high voidage (97%) matrix material that can be cut to desired geometrical shapes. Cytopore and RVC were combined to cylindrical composites (25mm 10mm) fitted inside chromatography columns. The composite combined the advantageous properties of both its constituents, making it suitable for column chromatography. The composite could withstand very high flow rates without compaction of the bed (>25column volumes/min; 4000cmh(-1)). Chromatography runs with tracers showed a low HETP value (0.3mm), suggesting that pore flow was in operation. The dynamic binding capacities (10% breakthrough) per gram of dry weight Cytopore were determined for several compounds including DNA and RNA and were found to be 240-370mg/g. The composite was used to isolate pUC 18-type plasmids from a cleared alkaline lysate in a good yield. Confocal microscopy studies showed that plasmids were bound not only to the surface of the Cytopore material but also within the matrix walls, thus offering an explanation to the very high binding capacities observed. The concept of using a composite prepared from a mechanically weak, high-binding material and a strong scaffold material may be applied to other systems as well. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of chromatography. A
volume
1218
issue
31
pages
5235 - 5240
publisher
Elsevier
external identifiers
  • wos:000293432200025
  • pmid:21719019
  • scopus:79960112275
ISSN
1873-3778
DOI
10.1016/j.chroma.2011.06.001
language
English
LU publication?
yes
id
b1b59faf-3dfe-44ac-a0a7-18fcb0d106cb (old id 2059210)
date added to LUP
2011-07-27 08:39:45
date last changed
2017-01-01 05:22:15
@article{b1b59faf-3dfe-44ac-a0a7-18fcb0d106cb,
  abstract     = {Cytopore is a bead-shaped, macroporous and easily compressible cellulose-based anion-exchange material intended for cultivation of anchor-dependent animal cells. Reticulated vitreous carbon (RVC) is a strong, non-compressible, high voidage (97%) matrix material that can be cut to desired geometrical shapes. Cytopore and RVC were combined to cylindrical composites (25mm 10mm) fitted inside chromatography columns. The composite combined the advantageous properties of both its constituents, making it suitable for column chromatography. The composite could withstand very high flow rates without compaction of the bed (>25column volumes/min; 4000cmh(-1)). Chromatography runs with tracers showed a low HETP value (0.3mm), suggesting that pore flow was in operation. The dynamic binding capacities (10% breakthrough) per gram of dry weight Cytopore were determined for several compounds including DNA and RNA and were found to be 240-370mg/g. The composite was used to isolate pUC 18-type plasmids from a cleared alkaline lysate in a good yield. Confocal microscopy studies showed that plasmids were bound not only to the surface of the Cytopore material but also within the matrix walls, thus offering an explanation to the very high binding capacities observed. The concept of using a composite prepared from a mechanically weak, high-binding material and a strong scaffold material may be applied to other systems as well.},
  author       = {Tiainen, Peter and Rokebul Anower, M and Larsson, Per-Olof},
  issn         = {1873-3778},
  language     = {eng},
  number       = {31},
  pages        = {5235--5240},
  publisher    = {Elsevier},
  series       = {Journal of chromatography. A},
  title        = {High-capacity composite adsorbents for nucleic acids.},
  url          = {http://dx.doi.org/10.1016/j.chroma.2011.06.001},
  volume       = {1218},
  year         = {2011},
}