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IL-7 mediates Ebf-1-dependent lineage restriction in early lymphoid progenitors

Tsapogas, Panagiotis; Zandi, Sasan; Ahsberg, Josefine; Zetterblad, Jenny; Welinder, Eva LU ; Jonsson, Jan Ingvar; Månsson, Robert LU ; Qian, Hong and Sigvardsson, Mikael (2011) In Blood 118(5). p.1283-1290
Abstract
Deficiencies in the IL-7 signaling pathway result in severe disruptions of lymphoid development in adult mice. To understand more about how IL-7 deficiency impacts early lymphoid development, we have investigated lineage restriction events within the common lymphoid progenitor (CLP) compartment in IL-7 knockout mice. This revealed that although IL-7 deficiency had a minor impact on the development of LY6D(-) multipotent CLPs, the formation of the lineage restricted LY6D(+) CLP population was dramatically reduced. This was reflected in a low-level transcription of B-lineage genes as well as in a loss of functional B-cell commitment. The few Ly6D(+) CLPs developed in the absence of IL-7 displayed increased lineage plasticity and low... (More)
Deficiencies in the IL-7 signaling pathway result in severe disruptions of lymphoid development in adult mice. To understand more about how IL-7 deficiency impacts early lymphoid development, we have investigated lineage restriction events within the common lymphoid progenitor (CLP) compartment in IL-7 knockout mice. This revealed that although IL-7 deficiency had a minor impact on the development of LY6D(-) multipotent CLPs, the formation of the lineage restricted LY6D(+) CLP population was dramatically reduced. This was reflected in a low-level transcription of B-lineage genes as well as in a loss of functional B-cell commitment. The few Ly6D(+) CLPs developed in the absence of IL-7 displayed increased lineage plasticity and low expression of Ebf-1. Absence of Ebf-1 could be linked to increased plasticity because even though Ly6D(+) cells develop in Ebf-1-deficient mice, these cells retain both natural killer and dendritic cell potential. This reveals that IL-7 is essential for normal development of Ly6D(+) CLPs and that Ebf-1 is crucial for lineage restriction in early lymphoid progenitors. (Blood. 2011; 118(5):1283-1290) (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Blood
volume
118
issue
5
pages
1283 - 1290
publisher
American Society of Hematology
external identifiers
  • wos:000293510000020
  • scopus:80051573966
ISSN
1528-0020
DOI
10.1182/blood-2011-01-332189
language
English
LU publication?
yes
id
9df59362-2e1a-4968-a7e4-6d0361a5e87f (old id 2071687)
date added to LUP
2011-09-02 08:28:25
date last changed
2017-11-12 03:01:39
@article{9df59362-2e1a-4968-a7e4-6d0361a5e87f,
  abstract     = {Deficiencies in the IL-7 signaling pathway result in severe disruptions of lymphoid development in adult mice. To understand more about how IL-7 deficiency impacts early lymphoid development, we have investigated lineage restriction events within the common lymphoid progenitor (CLP) compartment in IL-7 knockout mice. This revealed that although IL-7 deficiency had a minor impact on the development of LY6D(-) multipotent CLPs, the formation of the lineage restricted LY6D(+) CLP population was dramatically reduced. This was reflected in a low-level transcription of B-lineage genes as well as in a loss of functional B-cell commitment. The few Ly6D(+) CLPs developed in the absence of IL-7 displayed increased lineage plasticity and low expression of Ebf-1. Absence of Ebf-1 could be linked to increased plasticity because even though Ly6D(+) cells develop in Ebf-1-deficient mice, these cells retain both natural killer and dendritic cell potential. This reveals that IL-7 is essential for normal development of Ly6D(+) CLPs and that Ebf-1 is crucial for lineage restriction in early lymphoid progenitors. (Blood. 2011; 118(5):1283-1290)},
  author       = {Tsapogas, Panagiotis and Zandi, Sasan and Ahsberg, Josefine and Zetterblad, Jenny and Welinder, Eva and Jonsson, Jan Ingvar and Månsson, Robert and Qian, Hong and Sigvardsson, Mikael},
  issn         = {1528-0020},
  language     = {eng},
  number       = {5},
  pages        = {1283--1290},
  publisher    = {American Society of Hematology},
  series       = {Blood},
  title        = {IL-7 mediates Ebf-1-dependent lineage restriction in early lymphoid progenitors},
  url          = {http://dx.doi.org/10.1182/blood-2011-01-332189},
  volume       = {118},
  year         = {2011},
}