Advanced

Genetic polymorphism of chemokine receptors CCR2 and CCR5 in Swedish cervical cancer patients

Zheng, Biying; Wiklund, Fredrik; Gharizadeh, Baback; Sadat, Mehdi; Gambelunghe, Giovanni; Hallmans, Goran; Dillner, Joakim LU ; Wallin, Keng-Ling and Ghaderi, Mehran (2006) In Anticancer research 26(5B). p.3669-3674
Abstract
Chemokines are chemotactic cytokines that orchestrate leukocyte trafficking in tissues, thus, playing an important role in regulation of immunological processes. The aim of this study was to investigate the association of human papillomavirus (HPV) infection and cervical cancer with two DNA polymorphisms of the chemokine receptors CCR5-Delta 32 and CCR2-64L The study material consisted of 50 cervical intraepithelial neoplasia (CIN) cases and 50 of age and sampling-date matched controls, 100 invasive cervix cancer cases and 100 of their corresponding matched disease-free controls. Pyrosequencing (TM) was employed to genotype the CCR2-64I polymorphism. CCR5-Delta 32 was genotyped using standard PCR fragment length analysis. The frequencies... (More)
Chemokines are chemotactic cytokines that orchestrate leukocyte trafficking in tissues, thus, playing an important role in regulation of immunological processes. The aim of this study was to investigate the association of human papillomavirus (HPV) infection and cervical cancer with two DNA polymorphisms of the chemokine receptors CCR5-Delta 32 and CCR2-64L The study material consisted of 50 cervical intraepithelial neoplasia (CIN) cases and 50 of age and sampling-date matched controls, 100 invasive cervix cancer cases and 100 of their corresponding matched disease-free controls. Pyrosequencing (TM) was employed to genotype the CCR2-64I polymorphism. CCR5-Delta 32 was genotyped using standard PCR fragment length analysis. The frequencies of CCR2 and CCR5 genotypes from 150 patients and 150 healthy controls were representative of the general population according to the Hardy-Weinberg equilibrium analysis. Risk association was computed with conditional logistic regression analysis. HPV-positive individuals with the rare CCR5 Delta 32/Delta 32 genotype have a risk of 4.58 (CI=0.40-52.64, p-valite=0.045) compare to HPV negative group. The Delta-32 mutation on the CCR locus is imperceptibly associated with increased risk of HPV infection. In total, cervical neoplasia was not associated with genetic polymorphism of CCR2 and CCR5. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cervical neoplasia, chemokine receptor, gene polymorphism, pyrosequencing
in
Anticancer research
volume
26
issue
5B
pages
3669 - 3674
publisher
International Institute of Cancer Research
external identifiers
  • wos:000241707500008
  • scopus:33750685971
ISSN
1791-7530
language
English
LU publication?
yes
id
208b820e-a5c9-479a-a250-cf3d2a865489 (old id 378540)
alternative location
http://www.iiar-anticancer.org/research/level3.php?issue=5B&volume=26&page=3669
date added to LUP
2007-10-21 09:03:31
date last changed
2018-11-21 20:06:35
@article{208b820e-a5c9-479a-a250-cf3d2a865489,
  abstract     = {Chemokines are chemotactic cytokines that orchestrate leukocyte trafficking in tissues, thus, playing an important role in regulation of immunological processes. The aim of this study was to investigate the association of human papillomavirus (HPV) infection and cervical cancer with two DNA polymorphisms of the chemokine receptors CCR5-Delta 32 and CCR2-64L The study material consisted of 50 cervical intraepithelial neoplasia (CIN) cases and 50 of age and sampling-date matched controls, 100 invasive cervix cancer cases and 100 of their corresponding matched disease-free controls. Pyrosequencing (TM) was employed to genotype the CCR2-64I polymorphism. CCR5-Delta 32 was genotyped using standard PCR fragment length analysis. The frequencies of CCR2 and CCR5 genotypes from 150 patients and 150 healthy controls were representative of the general population according to the Hardy-Weinberg equilibrium analysis. Risk association was computed with conditional logistic regression analysis. HPV-positive individuals with the rare CCR5 Delta 32/Delta 32 genotype have a risk of 4.58 (CI=0.40-52.64, p-valite=0.045) compare to HPV negative group. The Delta-32 mutation on the CCR locus is imperceptibly associated with increased risk of HPV infection. In total, cervical neoplasia was not associated with genetic polymorphism of CCR2 and CCR5.},
  author       = {Zheng, Biying and Wiklund, Fredrik and Gharizadeh, Baback and Sadat, Mehdi and Gambelunghe, Giovanni and Hallmans, Goran and Dillner, Joakim and Wallin, Keng-Ling and Ghaderi, Mehran},
  issn         = {1791-7530},
  keyword      = {cervical neoplasia,chemokine receptor,gene polymorphism,pyrosequencing},
  language     = {eng},
  number       = {5B},
  pages        = {3669--3674},
  publisher    = {International Institute of Cancer Research},
  series       = {Anticancer research},
  title        = {Genetic polymorphism of chemokine receptors CCR2 and CCR5 in Swedish cervical cancer patients},
  volume       = {26},
  year         = {2006},
}