In vivo cytochrome and alternative pathway respiration in leaves of Arabidopsis thaliana plants with altered alternative oxidase under different light conditions
(2011) In Plant, Cell and Environment 34(8). p.1373-1383- Abstract
- The in vivo activity of the alternative pathway (V-alt) has been studied using the oxygen isotope fractionation method in leaves of Arabidopsis thaliana modified for the expression of the AtAOX1a gene by anti-sense (AS-12) or overexpression (XX-2). Under non-stressful conditions, V-alt was similar in all plant lines regardless of its different alternative pathway capacities (V-alt). Total leaf respiration (V-t) and V-alt were directly related to growth light conditions while electron partitioning between the cytochrome pathway (CP) and alternative pathway (AP) was unchanged by light levels. Interestingly, the AP functioned at full capacity in anti-sense plants under both growth light conditions. The role of the AP in response to a high... (More)
- The in vivo activity of the alternative pathway (V-alt) has been studied using the oxygen isotope fractionation method in leaves of Arabidopsis thaliana modified for the expression of the AtAOX1a gene by anti-sense (AS-12) or overexpression (XX-2). Under non-stressful conditions, V-alt was similar in all plant lines regardless of its different alternative pathway capacities (V-alt). Total leaf respiration (V-t) and V-alt were directly related to growth light conditions while electron partitioning between the cytochrome pathway (CP) and alternative pathway (AP) was unchanged by light levels. Interestingly, the AP functioned at full capacity in anti-sense plants under both growth light conditions. The role of the AP in response to a high light stress induced by short-term high light treatment (HLT) was also studied. In wild type and XX-2, both CP and AP rates increased proportionally after HLT while in AS-12, where the AP was unable to increase its rate, the CP accommodated all the increase in respiration. The results obtained under high light stress suggest that flexibility in the response of the mitochondrial electron transport chain is involved in sustaining photosynthetic rates in response to this stress while the saturated AP in AS-12 plants may contribute to the observed increase in photoinhibition. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2092907
- author
- Florez-Sarasa, Igor ; Flexas, Jaume ; Rasmusson, Allan LU ; Umbach, Ann L. ; Siedow, James N. and Ribas-Carbo, Miquel
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- high light, oxygen isotope fractionation, photoinhibition, plant, mitochondria
- in
- Plant, Cell and Environment
- volume
- 34
- issue
- 8
- pages
- 1373 - 1383
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000292698900013
- scopus:79960265167
- pmid:21486306
- ISSN
- 0140-7791
- DOI
- 10.1111/j.1365-3040.2011.02337.x
- language
- English
- LU publication?
- yes
- id
- 4591e68e-b900-4cf7-afb2-7c2a23348e95 (old id 2092907)
- date added to LUP
- 2016-04-01 11:06:24
- date last changed
- 2022-02-25 08:32:37
@article{4591e68e-b900-4cf7-afb2-7c2a23348e95,
abstract = {{The in vivo activity of the alternative pathway (V-alt) has been studied using the oxygen isotope fractionation method in leaves of Arabidopsis thaliana modified for the expression of the AtAOX1a gene by anti-sense (AS-12) or overexpression (XX-2). Under non-stressful conditions, V-alt was similar in all plant lines regardless of its different alternative pathway capacities (V-alt). Total leaf respiration (V-t) and V-alt were directly related to growth light conditions while electron partitioning between the cytochrome pathway (CP) and alternative pathway (AP) was unchanged by light levels. Interestingly, the AP functioned at full capacity in anti-sense plants under both growth light conditions. The role of the AP in response to a high light stress induced by short-term high light treatment (HLT) was also studied. In wild type and XX-2, both CP and AP rates increased proportionally after HLT while in AS-12, where the AP was unable to increase its rate, the CP accommodated all the increase in respiration. The results obtained under high light stress suggest that flexibility in the response of the mitochondrial electron transport chain is involved in sustaining photosynthetic rates in response to this stress while the saturated AP in AS-12 plants may contribute to the observed increase in photoinhibition.}},
author = {{Florez-Sarasa, Igor and Flexas, Jaume and Rasmusson, Allan and Umbach, Ann L. and Siedow, James N. and Ribas-Carbo, Miquel}},
issn = {{0140-7791}},
keywords = {{high light; oxygen isotope fractionation; photoinhibition; plant; mitochondria}},
language = {{eng}},
number = {{8}},
pages = {{1373--1383}},
publisher = {{Wiley-Blackwell}},
series = {{Plant, Cell and Environment}},
title = {{In vivo cytochrome and alternative pathway respiration in leaves of Arabidopsis thaliana plants with altered alternative oxidase under different light conditions}},
url = {{http://dx.doi.org/10.1111/j.1365-3040.2011.02337.x}},
doi = {{10.1111/j.1365-3040.2011.02337.x}},
volume = {{34}},
year = {{2011}},
}