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In vivo cytochrome and alternative pathway respiration in leaves of Arabidopsis thaliana plants with altered alternative oxidase under different light conditions

Florez-Sarasa, Igor; Flexas, Jaume; Rasmusson, Allan LU ; Umbach, Ann L.; Siedow, James N. and Ribas-Carbo, Miquel (2011) In Plant, Cell and Environment 34(8). p.1373-1383
Abstract
The in vivo activity of the alternative pathway (V-alt) has been studied using the oxygen isotope fractionation method in leaves of Arabidopsis thaliana modified for the expression of the AtAOX1a gene by anti-sense (AS-12) or overexpression (XX-2). Under non-stressful conditions, V-alt was similar in all plant lines regardless of its different alternative pathway capacities (V-alt). Total leaf respiration (V-t) and V-alt were directly related to growth light conditions while electron partitioning between the cytochrome pathway (CP) and alternative pathway (AP) was unchanged by light levels. Interestingly, the AP functioned at full capacity in anti-sense plants under both growth light conditions. The role of the AP in response to a high... (More)
The in vivo activity of the alternative pathway (V-alt) has been studied using the oxygen isotope fractionation method in leaves of Arabidopsis thaliana modified for the expression of the AtAOX1a gene by anti-sense (AS-12) or overexpression (XX-2). Under non-stressful conditions, V-alt was similar in all plant lines regardless of its different alternative pathway capacities (V-alt). Total leaf respiration (V-t) and V-alt were directly related to growth light conditions while electron partitioning between the cytochrome pathway (CP) and alternative pathway (AP) was unchanged by light levels. Interestingly, the AP functioned at full capacity in anti-sense plants under both growth light conditions. The role of the AP in response to a high light stress induced by short-term high light treatment (HLT) was also studied. In wild type and XX-2, both CP and AP rates increased proportionally after HLT while in AS-12, where the AP was unable to increase its rate, the CP accommodated all the increase in respiration. The results obtained under high light stress suggest that flexibility in the response of the mitochondrial electron transport chain is involved in sustaining photosynthetic rates in response to this stress while the saturated AP in AS-12 plants may contribute to the observed increase in photoinhibition. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
high light, oxygen isotope fractionation, photoinhibition, plant, mitochondria
in
Plant, Cell and Environment
volume
34
issue
8
pages
1373 - 1383
publisher
Wiley-Blackwell
external identifiers
  • wos:000292698900013
  • scopus:79960265167
ISSN
0140-7791
DOI
10.1111/j.1365-3040.2011.02337.x
language
English
LU publication?
yes
id
4591e68e-b900-4cf7-afb2-7c2a23348e95 (old id 2092907)
date added to LUP
2011-08-25 12:46:12
date last changed
2017-09-24 03:24:16
@article{4591e68e-b900-4cf7-afb2-7c2a23348e95,
  abstract     = {The in vivo activity of the alternative pathway (V-alt) has been studied using the oxygen isotope fractionation method in leaves of Arabidopsis thaliana modified for the expression of the AtAOX1a gene by anti-sense (AS-12) or overexpression (XX-2). Under non-stressful conditions, V-alt was similar in all plant lines regardless of its different alternative pathway capacities (V-alt). Total leaf respiration (V-t) and V-alt were directly related to growth light conditions while electron partitioning between the cytochrome pathway (CP) and alternative pathway (AP) was unchanged by light levels. Interestingly, the AP functioned at full capacity in anti-sense plants under both growth light conditions. The role of the AP in response to a high light stress induced by short-term high light treatment (HLT) was also studied. In wild type and XX-2, both CP and AP rates increased proportionally after HLT while in AS-12, where the AP was unable to increase its rate, the CP accommodated all the increase in respiration. The results obtained under high light stress suggest that flexibility in the response of the mitochondrial electron transport chain is involved in sustaining photosynthetic rates in response to this stress while the saturated AP in AS-12 plants may contribute to the observed increase in photoinhibition.},
  author       = {Florez-Sarasa, Igor and Flexas, Jaume and Rasmusson, Allan and Umbach, Ann L. and Siedow, James N. and Ribas-Carbo, Miquel},
  issn         = {0140-7791},
  keyword      = {high light,oxygen isotope fractionation,photoinhibition,plant,mitochondria},
  language     = {eng},
  number       = {8},
  pages        = {1373--1383},
  publisher    = {Wiley-Blackwell},
  series       = {Plant, Cell and Environment},
  title        = {In vivo cytochrome and alternative pathway respiration in leaves of Arabidopsis thaliana plants with altered alternative oxidase under different light conditions},
  url          = {http://dx.doi.org/10.1111/j.1365-3040.2011.02337.x},
  volume       = {34},
  year         = {2011},
}