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Coreceptor usage of primary HIV type 1 isolates obtained from different lymph node subsets

Casper, C; Mild, Mattias LU ; Jansson, Marianne LU ; Karlsson, A; Holmberg, V; Bratt, G; van Paaschen, H; Biberfeld, P; Bjorndal, A and Albert, J, et al. (2005) In AIDS Research and Human Retroviruses 21(12). p.1003-1010
Abstract
Biological characteristics of virus quantitatively rescued from different cell types present in lymph nodes of HIV-1-infected individuals in various stages of their disease were determined, not including patients with AIDS defining illness. Viruses were obtained by cocultivation with donor monocyte-derived macrophages and T-lymphocytes and their biological phenotype compared to viruses obtained from the peripheral blood mononuclear cells of the same patient. The biological phenotype was determined on established cell lines (U937-2, CEM, and MT-2) and on the U87.CD4 coreceptor indicator cell lines and variable region 3 (V3) of the envelope was subjected to direct sequencing. All isolates obtained from lymph node subsets used CCR5 as... (More)
Biological characteristics of virus quantitatively rescued from different cell types present in lymph nodes of HIV-1-infected individuals in various stages of their disease were determined, not including patients with AIDS defining illness. Viruses were obtained by cocultivation with donor monocyte-derived macrophages and T-lymphocytes and their biological phenotype compared to viruses obtained from the peripheral blood mononuclear cells of the same patient. The biological phenotype was determined on established cell lines (U937-2, CEM, and MT-2) and on the U87.CD4 coreceptor indicator cell lines and variable region 3 (V3) of the envelope was subjected to direct sequencing. All isolates obtained from lymph node subsets used CCR5 as coreceptor. Furthermore, these viruses were also sensitive to inhibition by beta-chemokines as analyzed for viruses of one patient. All 12 V3 regions showed a unique sequence indicating compartmentalization within each patient. The biological phenotype of CCR5-dependent (R5) HIV-1 isolates obtained from PBMC resembles the phenotype of viruses isolated from different lymph node cell subsets. (Less)
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publication status
published
subject
in
AIDS Research and Human Retroviruses
volume
21
issue
12
pages
1003 - 1010
publisher
Mary Ann Liebert, Inc.
external identifiers
  • pmid:16379603
  • wos:000234570100004
  • scopus:30344455310
ISSN
1931-8405
DOI
10.1089/aid.2005.21.1003
language
English
LU publication?
yes
id
1bfaf06c-78c4-46df-8a0d-48c872f2ddb8 (old id 210046)
date added to LUP
2007-08-07 11:35:42
date last changed
2017-01-01 04:57:28
@article{1bfaf06c-78c4-46df-8a0d-48c872f2ddb8,
  abstract     = {Biological characteristics of virus quantitatively rescued from different cell types present in lymph nodes of HIV-1-infected individuals in various stages of their disease were determined, not including patients with AIDS defining illness. Viruses were obtained by cocultivation with donor monocyte-derived macrophages and T-lymphocytes and their biological phenotype compared to viruses obtained from the peripheral blood mononuclear cells of the same patient. The biological phenotype was determined on established cell lines (U937-2, CEM, and MT-2) and on the U87.CD4 coreceptor indicator cell lines and variable region 3 (V3) of the envelope was subjected to direct sequencing. All isolates obtained from lymph node subsets used CCR5 as coreceptor. Furthermore, these viruses were also sensitive to inhibition by beta-chemokines as analyzed for viruses of one patient. All 12 V3 regions showed a unique sequence indicating compartmentalization within each patient. The biological phenotype of CCR5-dependent (R5) HIV-1 isolates obtained from PBMC resembles the phenotype of viruses isolated from different lymph node cell subsets.},
  author       = {Casper, C and Mild, Mattias and Jansson, Marianne and Karlsson, A and Holmberg, V and Bratt, G and van Paaschen, H and Biberfeld, P and Bjorndal, A and Albert, J and Popovic, M and Fenyö, Eva Maria},
  issn         = {1931-8405},
  language     = {eng},
  number       = {12},
  pages        = {1003--1010},
  publisher    = {Mary Ann Liebert, Inc.},
  series       = {AIDS Research and Human Retroviruses},
  title        = {Coreceptor usage of primary HIV type 1 isolates obtained from different lymph node subsets},
  url          = {http://dx.doi.org/10.1089/aid.2005.21.1003},
  volume       = {21},
  year         = {2005},
}