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Expression of inducible nitric oxide synthase in trigeminal ganglion cells during culture

Jansen-Olesen, I; Zhou, Mingfang LU ; Zinck, T; Xu, Cang-Bao LU and Edvinsson, Lars LU (2005) In Basic & Clinical Pharmacology & Toxicology 97(6). p.355-363
Abstract
Nitric oxide (NO) is an important signalling molecule that has been suggested to be a key molecule for induction and maintenance of migraine attacks based on clinical studies, animal experimental studies and the expression of nitric oxide synthase (NOS) immuno reactivity within the trigeminovascular system. Sensitisation of the trigeminal system including the trigeminal ganglia neurones is believed to be involved in the pathway leading to migraine pain. In the present study, the NOS expression in rat primary trigeminal ganglia neurones was examined at different time points using immuno-cytochemistry, reverse transcriptase polymerase chain reaction (RT-PCR) and Western blotting. In trigeminal ganglia cells not subjected to culture,... (More)
Nitric oxide (NO) is an important signalling molecule that has been suggested to be a key molecule for induction and maintenance of migraine attacks based on clinical studies, animal experimental studies and the expression of nitric oxide synthase (NOS) immuno reactivity within the trigeminovascular system. Sensitisation of the trigeminal system including the trigeminal ganglia neurones is believed to be involved in the pathway leading to migraine pain. In the present study, the NOS expression in rat primary trigeminal ganglia neurones was examined at different time points using immuno-cytochemistry, reverse transcriptase polymerase chain reaction (RT-PCR) and Western blotting. In trigeminal ganglia cells not subjected to culture, endothelial (e) and neuronal (n) but not inducible (i) NOS mRNA and protein were detected. Culture of rat neurotics resulted in a rapid axonal Outgrowth of NOS positive fibres. At 12, 24 and 48 hr of culture, NOS immunoreactivity was detected in medium-sized trigeminal ganglia cells. Western blotting and RT-PCR revealed an up-regulation of inducible iNOS expression during Culture. However, after Culture only low levels of eNOS protein was found while no eNOS and nNOS mRNA and protein could be detected. The data Suggest that iNOS expression may be a molecular mechanism mediating the adaptive response of trigeminal ganglia cells to the serum free stressful stimulus the Culture environment provides. It may act as a cellular signalling molecule that is expressed after cell activation. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Basic & Clinical Pharmacology & Toxicology
volume
97
issue
6
pages
355 - 363
publisher
Wiley-Blackwell
external identifiers
  • pmid:16364050
  • wos:000234380900004
  • scopus:29744467953
ISSN
1742-7843
language
English
LU publication?
yes
id
383ab392-bf44-483b-b802-8c23907ac884 (old id 210317)
date added to LUP
2007-08-10 13:52:15
date last changed
2017-01-01 04:38:26
@article{383ab392-bf44-483b-b802-8c23907ac884,
  abstract     = {Nitric oxide (NO) is an important signalling molecule that has been suggested to be a key molecule for induction and maintenance of migraine attacks based on clinical studies, animal experimental studies and the expression of nitric oxide synthase (NOS) immuno reactivity within the trigeminovascular system. Sensitisation of the trigeminal system including the trigeminal ganglia neurones is believed to be involved in the pathway leading to migraine pain. In the present study, the NOS expression in rat primary trigeminal ganglia neurones was examined at different time points using immuno-cytochemistry, reverse transcriptase polymerase chain reaction (RT-PCR) and Western blotting. In trigeminal ganglia cells not subjected to culture, endothelial (e) and neuronal (n) but not inducible (i) NOS mRNA and protein were detected. Culture of rat neurotics resulted in a rapid axonal Outgrowth of NOS positive fibres. At 12, 24 and 48 hr of culture, NOS immunoreactivity was detected in medium-sized trigeminal ganglia cells. Western blotting and RT-PCR revealed an up-regulation of inducible iNOS expression during Culture. However, after Culture only low levels of eNOS protein was found while no eNOS and nNOS mRNA and protein could be detected. The data Suggest that iNOS expression may be a molecular mechanism mediating the adaptive response of trigeminal ganglia cells to the serum free stressful stimulus the Culture environment provides. It may act as a cellular signalling molecule that is expressed after cell activation.},
  author       = {Jansen-Olesen, I and Zhou, Mingfang and Zinck, T and Xu, Cang-Bao and Edvinsson, Lars},
  issn         = {1742-7843},
  language     = {eng},
  number       = {6},
  pages        = {355--363},
  publisher    = {Wiley-Blackwell},
  series       = {Basic & Clinical Pharmacology & Toxicology},
  title        = {Expression of inducible nitric oxide synthase in trigeminal ganglion cells during culture},
  volume       = {97},
  year         = {2005},
}