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Gene expression analysis suggests that EBF-1 and PPAR gamma 2 induce adipogenesis of NIH-3T3 cells with similar efficiency and kinetics

Akerblad, P; Månsson, Robert LU ; Lagergren, Anna LU ; Westerlund, S; Basta, B; Lind, U; Thelin, A; Gisler, Ramiro LU ; Liberg, David LU and Nelander, S, et al. (2005) In Physiological Genomics 23(2). p.206-216
Abstract
Differentiation of multipotent mesenchymal stem cells into lipid-accumulating adipocytes is a physiological process induced by transcription factors in combination with hormonal stimulation. We have used Affymetrix microarrays to compare the adipogenic differentiation pathways of NIH-3T3 fibroblasts induced to undergo in vitro differentiation by ectopic expression of early B cell factor (EBF)-1 or peroxisome proliferator-activated receptor (PPAR)gamma 2. These experiments revealed that commitment to the adipogenic pathway in the NIH-3T3 cells was not reflected in gene expression until 4 days after induction of differentiation. Furthermore, gene expression patterns at the earlier time points after stimulation indicated that EBF-1 and PPAR... (More)
Differentiation of multipotent mesenchymal stem cells into lipid-accumulating adipocytes is a physiological process induced by transcription factors in combination with hormonal stimulation. We have used Affymetrix microarrays to compare the adipogenic differentiation pathways of NIH-3T3 fibroblasts induced to undergo in vitro differentiation by ectopic expression of early B cell factor (EBF)-1 or peroxisome proliferator-activated receptor (PPAR)gamma 2. These experiments revealed that commitment to the adipogenic pathway in the NIH-3T3 cells was not reflected in gene expression until 4 days after induction of differentiation. Furthermore, gene expression patterns at the earlier time points after stimulation indicated that EBF-1 and PPAR gamma 2 induced different sets of genes, while the similarities increased upon differentiation, and that several genes linked to adipocyte differentiation were also transiently induced in the vector-transduced cells. These data suggest that the initial activation of genes associated with adipocyte development is independent of commitment to the adipogenic pathway and that EBF-1 and PPAR gamma 2 induce adipocyte differentiation with comparable kinetics and efficiency. (Less)
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publication status
published
subject
keywords
early B cell factor, peroxisome proliferator-activated, adipogenesis, receptor-gamma 2
in
Physiological Genomics
volume
23
issue
2
pages
206 - 216
publisher
American Physiological Society
external identifiers
  • wos:000234086200009
  • scopus:33646898028
ISSN
1094-8341
DOI
10.1152/physiolgenomics.00015.2005
language
English
LU publication?
yes
id
725e1f56-b45f-431d-ab4d-856628d6af72 (old id 210428)
date added to LUP
2007-08-14 08:16:20
date last changed
2017-10-01 03:55:56
@article{725e1f56-b45f-431d-ab4d-856628d6af72,
  abstract     = {Differentiation of multipotent mesenchymal stem cells into lipid-accumulating adipocytes is a physiological process induced by transcription factors in combination with hormonal stimulation. We have used Affymetrix microarrays to compare the adipogenic differentiation pathways of NIH-3T3 fibroblasts induced to undergo in vitro differentiation by ectopic expression of early B cell factor (EBF)-1 or peroxisome proliferator-activated receptor (PPAR)gamma 2. These experiments revealed that commitment to the adipogenic pathway in the NIH-3T3 cells was not reflected in gene expression until 4 days after induction of differentiation. Furthermore, gene expression patterns at the earlier time points after stimulation indicated that EBF-1 and PPAR gamma 2 induced different sets of genes, while the similarities increased upon differentiation, and that several genes linked to adipocyte differentiation were also transiently induced in the vector-transduced cells. These data suggest that the initial activation of genes associated with adipocyte development is independent of commitment to the adipogenic pathway and that EBF-1 and PPAR gamma 2 induce adipocyte differentiation with comparable kinetics and efficiency.},
  author       = {Akerblad, P and Månsson, Robert and Lagergren, Anna and Westerlund, S and Basta, B and Lind, U and Thelin, A and Gisler, Ramiro and Liberg, David and Nelander, S and Bamberg, K and Sigvardsson, Mikael},
  issn         = {1094-8341},
  keyword      = {early B cell factor,peroxisome proliferator-activated,adipogenesis,receptor-gamma 2},
  language     = {eng},
  number       = {2},
  pages        = {206--216},
  publisher    = {American Physiological Society},
  series       = {Physiological Genomics},
  title        = {Gene expression analysis suggests that EBF-1 and PPAR gamma 2 induce adipogenesis of NIH-3T3 cells with similar efficiency and kinetics},
  url          = {http://dx.doi.org/10.1152/physiolgenomics.00015.2005},
  volume       = {23},
  year         = {2005},
}