Gene expression analysis suggests that EBF-1 and PPAR gamma 2 induce adipogenesis of NIH-3T3 cells with similar efficiency and kinetics
(2005) In Physiological Genomics 23(2). p.206-216- Abstract
- Differentiation of multipotent mesenchymal stem cells into lipid-accumulating adipocytes is a physiological process induced by transcription factors in combination with hormonal stimulation. We have used Affymetrix microarrays to compare the adipogenic differentiation pathways of NIH-3T3 fibroblasts induced to undergo in vitro differentiation by ectopic expression of early B cell factor (EBF)-1 or peroxisome proliferator-activated receptor (PPAR)gamma 2. These experiments revealed that commitment to the adipogenic pathway in the NIH-3T3 cells was not reflected in gene expression until 4 days after induction of differentiation. Furthermore, gene expression patterns at the earlier time points after stimulation indicated that EBF-1 and PPAR... (More)
- Differentiation of multipotent mesenchymal stem cells into lipid-accumulating adipocytes is a physiological process induced by transcription factors in combination with hormonal stimulation. We have used Affymetrix microarrays to compare the adipogenic differentiation pathways of NIH-3T3 fibroblasts induced to undergo in vitro differentiation by ectopic expression of early B cell factor (EBF)-1 or peroxisome proliferator-activated receptor (PPAR)gamma 2. These experiments revealed that commitment to the adipogenic pathway in the NIH-3T3 cells was not reflected in gene expression until 4 days after induction of differentiation. Furthermore, gene expression patterns at the earlier time points after stimulation indicated that EBF-1 and PPAR gamma 2 induced different sets of genes, while the similarities increased upon differentiation, and that several genes linked to adipocyte differentiation were also transiently induced in the vector-transduced cells. These data suggest that the initial activation of genes associated with adipocyte development is independent of commitment to the adipogenic pathway and that EBF-1 and PPAR gamma 2 induce adipocyte differentiation with comparable kinetics and efficiency. (Less)
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https://lup.lub.lu.se/record/210428
- author
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- early B cell factor, peroxisome proliferator-activated, adipogenesis, receptor-gamma 2
- in
- Physiological Genomics
- volume
- 23
- issue
- 2
- pages
- 206 - 216
- publisher
- American Physiological Society
- external identifiers
-
- wos:000234086200009
- scopus:33646898028
- ISSN
- 1094-8341
- DOI
- 10.1152/physiolgenomics.00015.2005
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Hematopoietic Stem Cell Laboratory (013022012)
- id
- 725e1f56-b45f-431d-ab4d-856628d6af72 (old id 210428)
- date added to LUP
- 2016-04-01 12:29:13
- date last changed
- 2022-07-30 03:14:46
@article{725e1f56-b45f-431d-ab4d-856628d6af72, abstract = {{Differentiation of multipotent mesenchymal stem cells into lipid-accumulating adipocytes is a physiological process induced by transcription factors in combination with hormonal stimulation. We have used Affymetrix microarrays to compare the adipogenic differentiation pathways of NIH-3T3 fibroblasts induced to undergo in vitro differentiation by ectopic expression of early B cell factor (EBF)-1 or peroxisome proliferator-activated receptor (PPAR)gamma 2. These experiments revealed that commitment to the adipogenic pathway in the NIH-3T3 cells was not reflected in gene expression until 4 days after induction of differentiation. Furthermore, gene expression patterns at the earlier time points after stimulation indicated that EBF-1 and PPAR gamma 2 induced different sets of genes, while the similarities increased upon differentiation, and that several genes linked to adipocyte differentiation were also transiently induced in the vector-transduced cells. These data suggest that the initial activation of genes associated with adipocyte development is independent of commitment to the adipogenic pathway and that EBF-1 and PPAR gamma 2 induce adipocyte differentiation with comparable kinetics and efficiency.}}, author = {{Akerblad, P and Månsson, Robert and Lagergren, Anna and Westerlund, S and Basta, B and Lind, U and Thelin, A and Gisler, Ramiro and Liberg, David and Nelander, S and Bamberg, K and Sigvardsson, Mikael}}, issn = {{1094-8341}}, keywords = {{early B cell factor; peroxisome proliferator-activated; adipogenesis; receptor-gamma 2}}, language = {{eng}}, number = {{2}}, pages = {{206--216}}, publisher = {{American Physiological Society}}, series = {{Physiological Genomics}}, title = {{Gene expression analysis suggests that EBF-1 and PPAR gamma 2 induce adipogenesis of NIH-3T3 cells with similar efficiency and kinetics}}, url = {{http://dx.doi.org/10.1152/physiolgenomics.00015.2005}}, doi = {{10.1152/physiolgenomics.00015.2005}}, volume = {{23}}, year = {{2005}}, }