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Cystatin C modulates neurodegeneration and neurogenesis following status epilepticus in mouse

Pirttila, T J; Lukasiuk, K; Håkansson, Katarina LU ; Grubb, Anders LU ; Abrahamson, Magnus LU and Pitkanen, A (2005) In Neurobiology of Disease 20(2). p.241-253
Abstract
Brain damaging insults cause alterations in neuronal networks that trigger epileptogenesis, and eventually lead to the appearance of spontaneous seizures. The present experiments were designed to study the cellular expression and functions of a cysteine proteinase inhibitor, cystatin C, whose gene expression is previously shown to be upregulated in the rat hippocampus during status epilepticus (SE)induced epileptogenesis. The present data showed that the expression of cystatin C protein increased in the mouse hippocampus 7 days following SE and localized mainly to astrocytes and microglia. Acute neuronal death in the hippocampus at 24 h after SE was reduced in cystatin C-/- mice. Also, the basal level of neurogenesis in the subgranular... (More)
Brain damaging insults cause alterations in neuronal networks that trigger epileptogenesis, and eventually lead to the appearance of spontaneous seizures. The present experiments were designed to study the cellular expression and functions of a cysteine proteinase inhibitor, cystatin C, whose gene expression is previously shown to be upregulated in the rat hippocampus during status epilepticus (SE)induced epileptogenesis. The present data showed that the expression of cystatin C protein increased in the mouse hippocampus 7 days following SE and localized mainly to astrocytes and microglia. Acute neuronal death in the hippocampus at 24 h after SE was reduced in cystatin C-/- mice. Also, the basal level of neurogenesis in the subgranular layer of dentate gyros was decreased in cystatin C-/- mice compared to wildtype littermates. Interestingly, migration of newly born neurons within the granule cell layer was attenuated in cystatin C-/- mice. These data demonstrate that cystatin C has a role in neuronal death and neurogenesis during SE-induced network reorganization. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
neural stem cells, mice, knock-out, kainic acid, hippocampus, FGF-2, epileptogenesis, epilepsy, neurodegeneration, seizure
in
Neurobiology of Disease
volume
20
issue
2
pages
241 - 253
publisher
Elsevier
external identifiers
  • wos:000233096700008
  • pmid:16242633
  • scopus:23244448732
ISSN
0969-9961
DOI
10.1016/j.nbd.2005.03.006
language
English
LU publication?
yes
id
f01e79f7-f2fa-4ba5-81f4-5089b123cc3b (old id 213780)
date added to LUP
2007-08-07 17:24:57
date last changed
2017-07-23 03:42:24
@article{f01e79f7-f2fa-4ba5-81f4-5089b123cc3b,
  abstract     = {Brain damaging insults cause alterations in neuronal networks that trigger epileptogenesis, and eventually lead to the appearance of spontaneous seizures. The present experiments were designed to study the cellular expression and functions of a cysteine proteinase inhibitor, cystatin C, whose gene expression is previously shown to be upregulated in the rat hippocampus during status epilepticus (SE)induced epileptogenesis. The present data showed that the expression of cystatin C protein increased in the mouse hippocampus 7 days following SE and localized mainly to astrocytes and microglia. Acute neuronal death in the hippocampus at 24 h after SE was reduced in cystatin C-/- mice. Also, the basal level of neurogenesis in the subgranular layer of dentate gyros was decreased in cystatin C-/- mice compared to wildtype littermates. Interestingly, migration of newly born neurons within the granule cell layer was attenuated in cystatin C-/- mice. These data demonstrate that cystatin C has a role in neuronal death and neurogenesis during SE-induced network reorganization.},
  author       = {Pirttila, T J and Lukasiuk, K and Håkansson, Katarina and Grubb, Anders and Abrahamson, Magnus and Pitkanen, A},
  issn         = {0969-9961},
  keyword      = {neural stem cells,mice,knock-out,kainic acid,hippocampus,FGF-2,epileptogenesis,epilepsy,neurodegeneration,seizure},
  language     = {eng},
  number       = {2},
  pages        = {241--253},
  publisher    = {Elsevier},
  series       = {Neurobiology of Disease},
  title        = {Cystatin C modulates neurodegeneration and neurogenesis following status epilepticus in mouse},
  url          = {http://dx.doi.org/10.1016/j.nbd.2005.03.006},
  volume       = {20},
  year         = {2005},
}