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Palm tree peroxidase-based biosensor with unique characteristics for hydrogen peroxide monitoring

Alpeeva, IS; Nistor, Mihaela LU ; Castillo Leon, Jaime LU ; Csöregi, Elisabeth LU and Sakharova, IY (2005) In Biosensors & Bioelectronics 21(5). p.742-748
Abstract
Three amperometric enzyme electrodes have been constructed by adsorbing anionic royal palm tree peroxidase (RPTP), anionic sweet potato peroxidase (SPP), or cationic horseradish peroxidase (HRP-C) on spectroscopic graphite electrodes. The resulting H2O2-sensitive biosensors were characterized both in a flow injection system and in batch mode to evaluate their main bioelectrochemical parameters, such as pH dependency,I-max, K-M(app), detection limit, linear range, operational and storage stability. The obtained results showed a distinctly different behavior for the plant peroxidase electrodes, demonstrating uniquely superior characteristics of the RPTP-based sensors. The broader linear range observed for the RPTP-based biosensor is... (More)
Three amperometric enzyme electrodes have been constructed by adsorbing anionic royal palm tree peroxidase (RPTP), anionic sweet potato peroxidase (SPP), or cationic horseradish peroxidase (HRP-C) on spectroscopic graphite electrodes. The resulting H2O2-sensitive biosensors were characterized both in a flow injection system and in batch mode to evaluate their main bioelectrochemical parameters, such as pH dependency,I-max, K-M(app), detection limit, linear range, operational and storage stability. The obtained results showed a distinctly different behavior for the plant peroxidase electrodes, demonstrating uniquely superior characteristics of the RPTP-based sensors. The broader linear range observed for the RPTP-based biosensor is explained by a high stability of this enzyme in presence of H2O2. The higher storage and operational stability of RPTP-based biosensor as well as its capability to measure hydrogen peroxide under acidic conditions connect with an extremely high thermal and pH-stability of RPTP. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
sweet potato, horseradish, palm tree, peroxidase, biosensors, hydrogen, peroxide
in
Biosensors & Bioelectronics
volume
21
issue
5
pages
742 - 748
publisher
Elsevier
external identifiers
  • wos:000233154300008
  • pmid:16242613
  • scopus:26944481128
ISSN
1873-4235
DOI
10.1016/j.bios.2005.01.008
language
English
LU publication?
yes
id
38f55db1-9f6b-4636-af5f-7e8ca61d84b6 (old id 214038)
date added to LUP
2007-08-21 11:37:40
date last changed
2017-07-02 04:27:26
@article{38f55db1-9f6b-4636-af5f-7e8ca61d84b6,
  abstract     = {Three amperometric enzyme electrodes have been constructed by adsorbing anionic royal palm tree peroxidase (RPTP), anionic sweet potato peroxidase (SPP), or cationic horseradish peroxidase (HRP-C) on spectroscopic graphite electrodes. The resulting H2O2-sensitive biosensors were characterized both in a flow injection system and in batch mode to evaluate their main bioelectrochemical parameters, such as pH dependency,I-max, K-M(app), detection limit, linear range, operational and storage stability. The obtained results showed a distinctly different behavior for the plant peroxidase electrodes, demonstrating uniquely superior characteristics of the RPTP-based sensors. The broader linear range observed for the RPTP-based biosensor is explained by a high stability of this enzyme in presence of H2O2. The higher storage and operational stability of RPTP-based biosensor as well as its capability to measure hydrogen peroxide under acidic conditions connect with an extremely high thermal and pH-stability of RPTP.},
  author       = {Alpeeva, IS and Nistor, Mihaela and Castillo Leon, Jaime and Csöregi, Elisabeth and Sakharova, IY},
  issn         = {1873-4235},
  keyword      = {sweet potato,horseradish,palm tree,peroxidase,biosensors,hydrogen,peroxide},
  language     = {eng},
  number       = {5},
  pages        = {742--748},
  publisher    = {Elsevier},
  series       = {Biosensors & Bioelectronics},
  title        = {Palm tree peroxidase-based biosensor with unique characteristics for hydrogen peroxide monitoring},
  url          = {http://dx.doi.org/10.1016/j.bios.2005.01.008},
  volume       = {21},
  year         = {2005},
}