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Mathematical modeling and statistical analysis of calcium-regulated insulin granule exocytosis in beta-cells from mice and humans.

Pedersen, Morten Gram LU ; Cortese, Giuliana and Eliasson, Lena LU (2011) In Progress in Biophysics and Molecular Biology 107(2). p.257-264
Abstract
Insulin is released from pancreatic beta-cells as a result of Ca(2+)-evoked exocytosis of dense-core granules. Secretion is biphasic, which has been suggested to correspond to the release of different granule pools. Here we review and carefully reanalyze previously published patch-clamp data on depolarization-evoked Ca(2+)-currents and corresponding capacitance measurements. Using a statistical mixed-effects model, we show that the data indicate that pool depletion is negligible in response to short depolarizations in mouse beta-cells. We then review mathematical models of granule dynamics and exocytosis in rodent beta-cells and present a mathematical description of Ca(2+)-evoked exocytosis in human beta-cells, which show clear differences... (More)
Insulin is released from pancreatic beta-cells as a result of Ca(2+)-evoked exocytosis of dense-core granules. Secretion is biphasic, which has been suggested to correspond to the release of different granule pools. Here we review and carefully reanalyze previously published patch-clamp data on depolarization-evoked Ca(2+)-currents and corresponding capacitance measurements. Using a statistical mixed-effects model, we show that the data indicate that pool depletion is negligible in response to short depolarizations in mouse beta-cells. We then review mathematical models of granule dynamics and exocytosis in rodent beta-cells and present a mathematical description of Ca(2+)-evoked exocytosis in human beta-cells, which show clear differences to their rodent counterparts. The model suggests that L- and P/Q-type Ca(2+)-channels are involved to a similar degree in exocytosis during electrical activity in human beta-cells. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Progress in Biophysics and Molecular Biology
volume
107
issue
2
pages
257 - 264
publisher
Elsevier
external identifiers
  • wos:000297489800006
  • pmid:21839108
  • scopus:82455199164
ISSN
1873-1732
DOI
10.1016/j.pbiomolbio.2011.07.012
language
English
LU publication?
yes
id
e76f9bb5-4bfd-441e-b454-af132d72df21 (old id 2151103)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21839108?dopt=Abstract
date added to LUP
2011-09-04 14:46:47
date last changed
2017-08-06 03:09:35
@article{e76f9bb5-4bfd-441e-b454-af132d72df21,
  abstract     = {Insulin is released from pancreatic beta-cells as a result of Ca(2+)-evoked exocytosis of dense-core granules. Secretion is biphasic, which has been suggested to correspond to the release of different granule pools. Here we review and carefully reanalyze previously published patch-clamp data on depolarization-evoked Ca(2+)-currents and corresponding capacitance measurements. Using a statistical mixed-effects model, we show that the data indicate that pool depletion is negligible in response to short depolarizations in mouse beta-cells. We then review mathematical models of granule dynamics and exocytosis in rodent beta-cells and present a mathematical description of Ca(2+)-evoked exocytosis in human beta-cells, which show clear differences to their rodent counterparts. The model suggests that L- and P/Q-type Ca(2+)-channels are involved to a similar degree in exocytosis during electrical activity in human beta-cells.},
  author       = {Pedersen, Morten Gram and Cortese, Giuliana and Eliasson, Lena},
  issn         = {1873-1732},
  language     = {eng},
  number       = {2},
  pages        = {257--264},
  publisher    = {Elsevier},
  series       = {Progress in Biophysics and Molecular Biology},
  title        = {Mathematical modeling and statistical analysis of calcium-regulated insulin granule exocytosis in beta-cells from mice and humans.},
  url          = {http://dx.doi.org/10.1016/j.pbiomolbio.2011.07.012},
  volume       = {107},
  year         = {2011},
}