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Novel anti-apoptotic effect of the retinoblastoma protein: implications for polyamine analogue toxicity.

Johansson, Veronica LU ; Thuvesson, Iréne LU ; Alm, Kersti LU and Oredsson, Stina LU (2012) In Amino Acids 42. p.929-937
Abstract
The retinoblastoma protein (pRb) pathway is frequently altered in breast cancer cells. pRb is involved in the regulation of cell proliferation and cell death. The breast cancer cell line L56Br-C1 does not express pRb and is extremely sensitive to treatment with the polyamine analogue N (1),N (11)-diethylnorspermine (DENSPM) which causes apoptosis. Polyamines are essential for the regulation of cell proliferation, cell differentiation and cell death. DENSPM depletes cells of polyamines, e.g., by inducing the activity of the polyamine catabolic enzyme spermidine/spermine N (1)-acetyltransferase (SSAT). In this study, L56Br-C1 cells were transfected with human pRb-cDNA. Overexpression of pRb inhibited DENSPM-induced cell death and... (More)
The retinoblastoma protein (pRb) pathway is frequently altered in breast cancer cells. pRb is involved in the regulation of cell proliferation and cell death. The breast cancer cell line L56Br-C1 does not express pRb and is extremely sensitive to treatment with the polyamine analogue N (1),N (11)-diethylnorspermine (DENSPM) which causes apoptosis. Polyamines are essential for the regulation of cell proliferation, cell differentiation and cell death. DENSPM depletes cells of polyamines, e.g., by inducing the activity of the polyamine catabolic enzyme spermidine/spermine N (1)-acetyltransferase (SSAT). In this study, L56Br-C1 cells were transfected with human pRb-cDNA. Overexpression of pRb inhibited DENSPM-induced cell death and DENSPM-induced SSAT activity. This suggests that the pRb protein level is a promising marker for polyamine depletion sensitivity and that there is a connection between pRb and the regulation of SSAT activity. We also show that SSAT protein levels and SSAT activity do not always correlate, suggesting that there is an unknown regulation of SSAT (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Amino Acids
volume
42
pages
929 - 937
publisher
Springer
external identifiers
  • wos:000299506000049
  • pmid:21809081
  • scopus:84861657647
ISSN
0939-4451
DOI
10.1007/s00726-011-1007-y
language
English
LU publication?
yes
id
38d0b841-32e1-44b2-93c7-406fde202871 (old id 2151616)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21809081?dopt=Abstract
date added to LUP
2011-09-04 16:55:16
date last changed
2017-01-01 07:29:25
@article{38d0b841-32e1-44b2-93c7-406fde202871,
  abstract     = {The retinoblastoma protein (pRb) pathway is frequently altered in breast cancer cells. pRb is involved in the regulation of cell proliferation and cell death. The breast cancer cell line L56Br-C1 does not express pRb and is extremely sensitive to treatment with the polyamine analogue N (1),N (11)-diethylnorspermine (DENSPM) which causes apoptosis. Polyamines are essential for the regulation of cell proliferation, cell differentiation and cell death. DENSPM depletes cells of polyamines, e.g., by inducing the activity of the polyamine catabolic enzyme spermidine/spermine N (1)-acetyltransferase (SSAT). In this study, L56Br-C1 cells were transfected with human pRb-cDNA. Overexpression of pRb inhibited DENSPM-induced cell death and DENSPM-induced SSAT activity. This suggests that the pRb protein level is a promising marker for polyamine depletion sensitivity and that there is a connection between pRb and the regulation of SSAT activity. We also show that SSAT protein levels and SSAT activity do not always correlate, suggesting that there is an unknown regulation of SSAT},
  author       = {Johansson, Veronica and Thuvesson, Iréne and Alm, Kersti and Oredsson, Stina},
  issn         = {0939-4451},
  language     = {eng},
  pages        = {929--937},
  publisher    = {Springer},
  series       = {Amino Acids},
  title        = {Novel anti-apoptotic effect of the retinoblastoma protein: implications for polyamine analogue toxicity.},
  url          = {http://dx.doi.org/10.1007/s00726-011-1007-y},
  volume       = {42},
  year         = {2012},
}