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Stimulation of blood mononuclear cells with bacterial virulence factors leads to the release of pro-coagulant and pro-inflammatory microparticles.

Oehmcke, Sonja LU ; Mörgelin, Matthias LU ; Malmström, Johan; Linder, Adam LU ; Chew, Michelle LU ; Thorlacius, Henrik LU and Herwald, Heiko LU (2012) In Cellular Microbiology 14. p.107-119
Abstract
Severe infectious diseases remain a major and life-threatening health problem. In serious cases a systemic activation of the coagulation cascade and hypovolemic shock are critical complications that are associated with high mortality rates. Here we report that blood mononuclear cells, stimulated with M1 protein of Streptococcus pyogenes or other bacterial virulence factors, produce not only pro-coagulant, but also pro-inflammatory microparticles (MPs). Our results also show that activation of the contact system on MPs contributes to these two effects. Phosphatidylserine (PS) plays an important role in these processes as its up-regulation on MPs allows an assembly and activation of the contact system. This in turn results in stabilization... (More)
Severe infectious diseases remain a major and life-threatening health problem. In serious cases a systemic activation of the coagulation cascade and hypovolemic shock are critical complications that are associated with high mortality rates. Here we report that blood mononuclear cells, stimulated with M1 protein of Streptococcus pyogenes or other bacterial virulence factors, produce not only pro-coagulant, but also pro-inflammatory microparticles (MPs). Our results also show that activation of the contact system on MPs contributes to these two effects. Phosphatidylserine (PS) plays an important role in these processes as its up-regulation on MPs allows an assembly and activation of the contact system. This in turn results in stabilization of the tissue factor-induced clot and a processing of high-molecular weight kininogen by plasma kallikrein followed by the release of bradykinin, a potent vascular mediator. Pro-coagulant monocyte-derived MPs were identified in plasma samples from septic patients and further analysis of MPs from these patients revealed that their pro-coagulant activity is dependent on the tissue factor- and contact system-driven pathway. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Cellular Microbiology
volume
14
pages
107 - 119
publisher
Wiley-Blackwell
external identifiers
  • wos:000298061800010
  • pmid:21951918
  • scopus:83655163674
ISSN
1462-5814
DOI
10.1111/j.1462-5822.2011.01705.x
language
English
LU publication?
yes
id
54e3a5e8-7149-4018-8836-63905e803d98 (old id 2168362)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21951918?dopt=Abstract
date added to LUP
2011-10-03 14:34:43
date last changed
2017-08-13 04:35:57
@article{54e3a5e8-7149-4018-8836-63905e803d98,
  abstract     = {Severe infectious diseases remain a major and life-threatening health problem. In serious cases a systemic activation of the coagulation cascade and hypovolemic shock are critical complications that are associated with high mortality rates. Here we report that blood mononuclear cells, stimulated with M1 protein of Streptococcus pyogenes or other bacterial virulence factors, produce not only pro-coagulant, but also pro-inflammatory microparticles (MPs). Our results also show that activation of the contact system on MPs contributes to these two effects. Phosphatidylserine (PS) plays an important role in these processes as its up-regulation on MPs allows an assembly and activation of the contact system. This in turn results in stabilization of the tissue factor-induced clot and a processing of high-molecular weight kininogen by plasma kallikrein followed by the release of bradykinin, a potent vascular mediator. Pro-coagulant monocyte-derived MPs were identified in plasma samples from septic patients and further analysis of MPs from these patients revealed that their pro-coagulant activity is dependent on the tissue factor- and contact system-driven pathway.},
  author       = {Oehmcke, Sonja and Mörgelin, Matthias and Malmström, Johan and Linder, Adam and Chew, Michelle and Thorlacius, Henrik and Herwald, Heiko},
  issn         = {1462-5814},
  language     = {eng},
  pages        = {107--119},
  publisher    = {Wiley-Blackwell},
  series       = {Cellular Microbiology},
  title        = {Stimulation of blood mononuclear cells with bacterial virulence factors leads to the release of pro-coagulant and pro-inflammatory microparticles.},
  url          = {http://dx.doi.org/10.1111/j.1462-5822.2011.01705.x},
  volume       = {14},
  year         = {2012},
}