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Spermatozoa DNA damage measured by sperm chromatin structure assay (SCSA) and birth characteristics in children conceived by IVF and ICSI.

Bungum, Mona LU ; Bungum, Leif LU ; Lynch, Kristian LU ; Wedlund, L; Humaidan, P and Giwercman, Aleksander LU (2012) In International journal of andrology 35(4). p.485-490
Abstract
High levels of spermatozoa DNA damage hinder fertility in vivo but not in vitro. It is a source of worry that following in vitro fertilization (IVF) spermatozoa DNA damage, if not repaired by the oocyte, might have a negative impact on the offspring. The aim of this study was to assess if a high spermatozoa DNA Fragmentation Index (DFI) is associated with alterations in birthweight (BW) and/or gestational length in IVF children. One hundred and thirty-one singleton pregnancies established by standard IVF or intracytoplasmic sperm injection (ICSI) were included in the study. DFI was measured by sperm chromatin structure assay (SCSA) in semen samples used for fertilization. DFI was categorized as low and high, using 20, 30, 40 and 50% as... (More)
High levels of spermatozoa DNA damage hinder fertility in vivo but not in vitro. It is a source of worry that following in vitro fertilization (IVF) spermatozoa DNA damage, if not repaired by the oocyte, might have a negative impact on the offspring. The aim of this study was to assess if a high spermatozoa DNA Fragmentation Index (DFI) is associated with alterations in birthweight (BW) and/or gestational length in IVF children. One hundred and thirty-one singleton pregnancies established by standard IVF or intracytoplasmic sperm injection (ICSI) were included in the study. DFI was measured by sperm chromatin structure assay (SCSA) in semen samples used for fertilization. DFI was categorized as low and high, using 20, 30, 40 and 50% as cut-off levels. Birthweight, gestational age, as well as gestational age adjusted BW score were used in a linear regression model as end points For none of the tested birth characteristics, statistically significant differences between the groups with low and high DFI were seen regardless of whether 20, 30, 40 or 50% were used as cut-off levels, both when the IVF and ICSI data were merged or analysed separately. Spermatozoa DNA damage as assessed by SCSA is not associated with BW or gestational length in IVF and ICSI children. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
International journal of andrology
volume
35
issue
4
pages
485 - 490
publisher
Wiley-Blackwell
external identifiers
  • wos:000306309100002
  • pmid:21950616
  • scopus:84863863307
ISSN
1365-2605
DOI
10.1111/j.1365-2605.2011.01222.x
language
English
LU publication?
yes
id
1439771c-12ae-4389-bfcc-c976835cd727 (old id 2168385)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21950616?dopt=Abstract
date added to LUP
2011-10-03 14:29:24
date last changed
2017-08-20 04:04:47
@article{1439771c-12ae-4389-bfcc-c976835cd727,
  abstract     = {High levels of spermatozoa DNA damage hinder fertility in vivo but not in vitro. It is a source of worry that following in vitro fertilization (IVF) spermatozoa DNA damage, if not repaired by the oocyte, might have a negative impact on the offspring. The aim of this study was to assess if a high spermatozoa DNA Fragmentation Index (DFI) is associated with alterations in birthweight (BW) and/or gestational length in IVF children. One hundred and thirty-one singleton pregnancies established by standard IVF or intracytoplasmic sperm injection (ICSI) were included in the study. DFI was measured by sperm chromatin structure assay (SCSA) in semen samples used for fertilization. DFI was categorized as low and high, using 20, 30, 40 and 50% as cut-off levels. Birthweight, gestational age, as well as gestational age adjusted BW score were used in a linear regression model as end points For none of the tested birth characteristics, statistically significant differences between the groups with low and high DFI were seen regardless of whether 20, 30, 40 or 50% were used as cut-off levels, both when the IVF and ICSI data were merged or analysed separately. Spermatozoa DNA damage as assessed by SCSA is not associated with BW or gestational length in IVF and ICSI children.},
  author       = {Bungum, Mona and Bungum, Leif and Lynch, Kristian and Wedlund, L and Humaidan, P and Giwercman, Aleksander},
  issn         = {1365-2605},
  language     = {eng},
  number       = {4},
  pages        = {485--490},
  publisher    = {Wiley-Blackwell},
  series       = {International journal of andrology},
  title        = {Spermatozoa DNA damage measured by sperm chromatin structure assay (SCSA) and birth characteristics in children conceived by IVF and ICSI.},
  url          = {http://dx.doi.org/10.1111/j.1365-2605.2011.01222.x},
  volume       = {35},
  year         = {2012},
}