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On-line Detection of Acetate Formation in Escherichia coli Cultures using Dissolved Oxygen Responses to Feed Transients

Åkesson, Mats; Nordberg Karlsson, Eva LU ; Hagander, Per LU ; Axelsson, Jan Peter and Tocaj, Anita (1999) In Biotechnology and Bioengineering 64(5). p.590-598
Abstract
Recombinant protein production in <i>Escherichia coli</i> can be significantly reduced by acetate accumulation. It is demonstrated that acetate production can be detected on-line with a standard dissolved oxygen sensor by superimposing short pulses to the substrate feed rate. Assuming that acetate formation is linked to a respiratory limitation, a model for dissolved oxygen responses to transients in substrate feed rate is derived. The model predicts a clear change in the character of the transient response when acetate formation starts. The predicted effect was verified in fed-batch cultivations of E. coli TOPP1 and E. coli BL21(DE3), both before and after induction of recombinant protein production. It was also observed that... (More)
Recombinant protein production in <i>Escherichia coli</i> can be significantly reduced by acetate accumulation. It is demonstrated that acetate production can be detected on-line with a standard dissolved oxygen sensor by superimposing short pulses to the substrate feed rate. Assuming that acetate formation is linked to a respiratory limitation, a model for dissolved oxygen responses to transients in substrate feed rate is derived. The model predicts a clear change in the character of the transient response when acetate formation starts. The predicted effect was verified in fed-batch cultivations of E. coli TOPP1 and E. coli BL21(DE3), both before and after induction of recombinant protein production. It was also observed that the critical specific glucose uptake rate, at which acetate formation starts, was significantly decreased after induction. On-line detection of acetate formation with a standard sensor opens up new possibilities for feedback control of substrate feeding. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
on-line detection, acetate, \emph{Escherichia coli}, dissolved oxygen, transients, mathematical model
in
Biotechnology and Bioengineering
volume
64
issue
5
pages
590 - 598
publisher
John Wiley & Sons
external identifiers
  • scopus:0345085733
ISSN
1097-0290
DOI
10.1002/(SICI)1097-0290(19990905)64:5<590::AID-BIT9>3.0.CO;2-T/abstract
language
English
LU publication?
yes
id
de6fcbe2-79c1-4731-8e3b-f7599b507cee (old id 2174874)
date added to LUP
2011-10-19 14:04:50
date last changed
2017-07-23 03:52:15
@article{de6fcbe2-79c1-4731-8e3b-f7599b507cee,
  abstract     = {Recombinant protein production in &lt;i&gt;Escherichia coli&lt;/i&gt; can be significantly reduced by acetate accumulation. It is demonstrated that acetate production can be detected on-line with a standard dissolved oxygen sensor by superimposing short pulses to the substrate feed rate. Assuming that acetate formation is linked to a respiratory limitation, a model for dissolved oxygen responses to transients in substrate feed rate is derived. The model predicts a clear change in the character of the transient response when acetate formation starts. The predicted effect was verified in fed-batch cultivations of E. coli TOPP1 and E. coli BL21(DE3), both before and after induction of recombinant protein production. It was also observed that the critical specific glucose uptake rate, at which acetate formation starts, was significantly decreased after induction. On-line detection of acetate formation with a standard sensor opens up new possibilities for feedback control of substrate feeding.},
  author       = {Åkesson, Mats and Nordberg Karlsson, Eva and Hagander, Per and Axelsson, Jan Peter and Tocaj, Anita},
  issn         = {1097-0290},
  keyword      = {on-line detection,acetate,\emph{Escherichia coli},dissolved oxygen,transients,mathematical model},
  language     = {eng},
  number       = {5},
  pages        = {590--598},
  publisher    = {John Wiley & Sons},
  series       = {Biotechnology and Bioengineering},
  title        = {On-line Detection of Acetate Formation in Escherichia coli Cultures using Dissolved Oxygen Responses to Feed Transients},
  url          = {http://dx.doi.org/10.1002/(SICI)1097-0290(19990905)64:5<590::AID-BIT9>3.0.CO;2-T/abstract},
  volume       = {64},
  year         = {1999},
}