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Fixation of microsporidian spores for electron microscopy

Larsson, Ronny LU (2005) In Journal of Invertebrate Pathology 90(1). p.47-50
Abstract
Fresh and frozen spores of the microsporidia Nosema apis and Nosema bombi were fixed using various fixatives at different times and temperatures. Paraformaldehyde and technical formaldehyde gave results comparable to or better than glutaraldehyde. Increased fixation temperature improved the fixation of spores from terrestrial hosts. Freezing did not destroy the cytology or the spore.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
temperature, paraformaldehyde, formaldehyde, microsporidia, spores
in
Journal of Invertebrate Pathology
volume
90
issue
1
pages
47 - 50
publisher
Elsevier
external identifiers
  • pmid:16112682
  • wos:000232727000006
  • scopus:26244458033
ISSN
0022-2011
DOI
10.1016/j.jip.2005.06.016
language
English
LU publication?
yes
id
c1ba5608-97eb-4501-b3a3-f7fe1758f500 (old id 218751)
date added to LUP
2007-08-13 13:11:25
date last changed
2017-01-01 05:11:52
@article{c1ba5608-97eb-4501-b3a3-f7fe1758f500,
  abstract     = {Fresh and frozen spores of the microsporidia Nosema apis and Nosema bombi were fixed using various fixatives at different times and temperatures. Paraformaldehyde and technical formaldehyde gave results comparable to or better than glutaraldehyde. Increased fixation temperature improved the fixation of spores from terrestrial hosts. Freezing did not destroy the cytology or the spore.},
  author       = {Larsson, Ronny},
  issn         = {0022-2011},
  keyword      = {temperature,paraformaldehyde,formaldehyde,microsporidia,spores},
  language     = {eng},
  number       = {1},
  pages        = {47--50},
  publisher    = {Elsevier},
  series       = {Journal of Invertebrate Pathology},
  title        = {Fixation of microsporidian spores for electron microscopy},
  url          = {http://dx.doi.org/10.1016/j.jip.2005.06.016},
  volume       = {90},
  year         = {2005},
}