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Coupling of permeabilized cells of Gluconobacter oxydans and Ralstonia eutropha for asymmetric ketone reduction using H(2) as reductant.

Rundbäck, Fabian LU ; Fidanoska, Milica LU and Adlercreutz, Patrick LU (2012) In Journal of Biotechnology 157. p.154-158
Abstract
A combined two-cell reaction system containing Gluconobacter oxydans and Ralstonia eutropha was evaluated with regard to asymmetric ketone reduction using H(2) as the reductant. Whole cells permeabilized by EDTA/toluene were used, and synthesis was performed in a biphasic aqueous/organic reaction medium. The two-cell system was compared with a system in which G. oxydans alone was used for both ketone reduction and cofactor regeneration, using an alcohol as co-substrate. The two-cell system exhibited almost twice the initial reaction rate of the single-cell system, a higher yield (75% vs. 48%) but slightly lower enantiomeric purity (93% vs. 98%) of the product (S)-2-octanol. The permeabilized R. eutropha cells are worth evaluating for... (More)
A combined two-cell reaction system containing Gluconobacter oxydans and Ralstonia eutropha was evaluated with regard to asymmetric ketone reduction using H(2) as the reductant. Whole cells permeabilized by EDTA/toluene were used, and synthesis was performed in a biphasic aqueous/organic reaction medium. The two-cell system was compared with a system in which G. oxydans alone was used for both ketone reduction and cofactor regeneration, using an alcohol as co-substrate. The two-cell system exhibited almost twice the initial reaction rate of the single-cell system, a higher yield (75% vs. 48%) but slightly lower enantiomeric purity (93% vs. 98%) of the product (S)-2-octanol. The permeabilized R. eutropha cells are worth evaluating for byproduct-free NADH regeneration in combination with other whole cell catalysts. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Biotechnology
volume
157
pages
154 - 158
publisher
Elsevier
external identifiers
  • wos:000298635200022
  • pmid:22001848
  • scopus:84855224161
ISSN
1873-4863
DOI
10.1016/j.jbiotec.2011.09.029
language
English
LU publication?
yes
id
54d283fc-3ab7-4e34-b8ac-15a49ff9dc89 (old id 2200476)
date added to LUP
2011-11-09 12:22:36
date last changed
2017-04-23 03:21:27
@article{54d283fc-3ab7-4e34-b8ac-15a49ff9dc89,
  abstract     = {A combined two-cell reaction system containing Gluconobacter oxydans and Ralstonia eutropha was evaluated with regard to asymmetric ketone reduction using H(2) as the reductant. Whole cells permeabilized by EDTA/toluene were used, and synthesis was performed in a biphasic aqueous/organic reaction medium. The two-cell system was compared with a system in which G. oxydans alone was used for both ketone reduction and cofactor regeneration, using an alcohol as co-substrate. The two-cell system exhibited almost twice the initial reaction rate of the single-cell system, a higher yield (75% vs. 48%) but slightly lower enantiomeric purity (93% vs. 98%) of the product (S)-2-octanol. The permeabilized R. eutropha cells are worth evaluating for byproduct-free NADH regeneration in combination with other whole cell catalysts.},
  author       = {Rundbäck, Fabian and Fidanoska, Milica and Adlercreutz, Patrick},
  issn         = {1873-4863},
  language     = {eng},
  pages        = {154--158},
  publisher    = {Elsevier},
  series       = {Journal of Biotechnology},
  title        = {Coupling of permeabilized cells of Gluconobacter oxydans and Ralstonia eutropha for asymmetric ketone reduction using H(2) as reductant.},
  url          = {http://dx.doi.org/10.1016/j.jbiotec.2011.09.029},
  volume       = {157},
  year         = {2012},
}