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Automated PreScan function for scanning fluorescently stained 2D-PAGE gels

Bäck, Peter LU ; Waldemarson, Sofia LU and James, Peter LU (2005) In Journal of Proteome Research 4(5). p.1511-1515
Abstract
To automate the acquisition of images from fluorescently stained gels, the power of the excitation laser(s) must be optimized for each sample to prevent spot saturation (or to allow unimportant spots to saturate) yet still retaining sensitivity. In this work, we describe the implementation and effectiveness of a pre-scan function in a robotic solution for the automation of 2D gel scanning.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
automation, image acquisition, 2D gel
in
Journal of Proteome Research
volume
4
issue
5
pages
1511 - 1515
publisher
The American Chemical Society
external identifiers
  • wos:000232579100010
  • scopus:26844525093
ISSN
1535-3893
DOI
10.1021/pr0500408
language
English
LU publication?
yes
id
0b724e52-171e-40da-8be2-d6d8de9dbdd2 (old id 220142)
date added to LUP
2007-08-02 11:07:55
date last changed
2017-01-01 04:30:18
@article{0b724e52-171e-40da-8be2-d6d8de9dbdd2,
  abstract     = {To automate the acquisition of images from fluorescently stained gels, the power of the excitation laser(s) must be optimized for each sample to prevent spot saturation (or to allow unimportant spots to saturate) yet still retaining sensitivity. In this work, we describe the implementation and effectiveness of a pre-scan function in a robotic solution for the automation of 2D gel scanning.},
  author       = {Bäck, Peter and Waldemarson, Sofia and James, Peter},
  issn         = {1535-3893},
  keyword      = {automation,image acquisition,2D gel},
  language     = {eng},
  number       = {5},
  pages        = {1511--1515},
  publisher    = {The American Chemical Society},
  series       = {Journal of Proteome Research},
  title        = {Automated PreScan function for scanning fluorescently stained 2D-PAGE gels},
  url          = {http://dx.doi.org/10.1021/pr0500408},
  volume       = {4},
  year         = {2005},
}