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BB rat Gimap gene expression in sorted lymphoid T and B cells

Moralejo, Daniel H.; Fuller, Jessica LU ; Rutledge, Elizabeth A.; Van Yserloo, Brian; Ettinger, Ruth A.; Jensen, Richard; Osborne, William; Kwitek, Anne and Lernmark, Åke LU (2011) In Life Sciences 89(19-20). p.748-754
Abstract
Aims: The Gimap gene family has been shown to be integral to T cell survival and development. A frameshift mutation in Gimap5, one of seven members of the Gimap family, results in lymphopenia and is a prerequisite for spontaneous type 1 diabetes (T1D) in the BioBreeding (BB) rat. While not contributing to lymphopenia, the Gimap family members proximal to Gimap5, encompassed within the Iddm39 quantitative trait locus (QTL), have been implicated in T1D. We hypothesized that expression of the Gimap family members within the Iddm39 QTL, during thymocyte development as well as in peripheral T and B cells contribute to T1D. Main methods: Cell sorted subpopulations were analyzed by quantitative real time (qRT) PCR. Key findings: Gimap4 expression... (More)
Aims: The Gimap gene family has been shown to be integral to T cell survival and development. A frameshift mutation in Gimap5, one of seven members of the Gimap family, results in lymphopenia and is a prerequisite for spontaneous type 1 diabetes (T1D) in the BioBreeding (BB) rat. While not contributing to lymphopenia, the Gimap family members proximal to Gimap5, encompassed within the Iddm39 quantitative trait locus (QTL), have been implicated in T1D. We hypothesized that expression of the Gimap family members within the Iddm39 QTL, during thymocyte development as well as in peripheral T and B cells contribute to T1D. Main methods: Cell sorted subpopulations were analyzed by quantitative real time (qRT) PCR. Key findings: Gimap4 expression was reduced in DR.(lyp/lyp) rat double negative, double positive and CD8 single positive (SP) thymocytes while expression of Gimap8, Gimap6, and Gimap7 was reduced only in CD8 SP thymocytes. Interestingly, expression of the entire Gimap gene family was reduced in DR.(lyp/lyp) rat peripheral T cells compared to non-lymphopenic, non-diabetic DR.(+/+) rats. With the exception of Gimap6. the Gimap family genes were not expressed in B cells from spleen and mesenteric lymph node (MLN). Expression of Gimap9 was only detected in hematopoietic cells of non B cell lineage such as macrophage, dendritic or NK cells. Significance: These results suggest that lack of the Gimap5 protein in the DR.(lyp/lyp) congenic rat was associated with impaired expression of the entire family of Gimap genes and may regulate T cell homeostasis in the peripheral lymphoid organs. (C) 2011 Elsevier Inc. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Gimap, Lymphopenia, Autoimmunity, Type 1 diabetes
in
Life Sciences
volume
89
issue
19-20
pages
748 - 754
publisher
Elsevier
external identifiers
  • wos:000296311800011
  • scopus:80054970804
ISSN
1879-0631
DOI
10.1016/j.lfs.2011.08.016
language
English
LU publication?
yes
id
3e9e5391-bcfd-4bd1-9e44-93f60dd2f212 (old id 2208154)
date added to LUP
2011-12-01 08:27:50
date last changed
2017-01-01 04:07:07
@article{3e9e5391-bcfd-4bd1-9e44-93f60dd2f212,
  abstract     = {Aims: The Gimap gene family has been shown to be integral to T cell survival and development. A frameshift mutation in Gimap5, one of seven members of the Gimap family, results in lymphopenia and is a prerequisite for spontaneous type 1 diabetes (T1D) in the BioBreeding (BB) rat. While not contributing to lymphopenia, the Gimap family members proximal to Gimap5, encompassed within the Iddm39 quantitative trait locus (QTL), have been implicated in T1D. We hypothesized that expression of the Gimap family members within the Iddm39 QTL, during thymocyte development as well as in peripheral T and B cells contribute to T1D. Main methods: Cell sorted subpopulations were analyzed by quantitative real time (qRT) PCR. Key findings: Gimap4 expression was reduced in DR.(lyp/lyp) rat double negative, double positive and CD8 single positive (SP) thymocytes while expression of Gimap8, Gimap6, and Gimap7 was reduced only in CD8 SP thymocytes. Interestingly, expression of the entire Gimap gene family was reduced in DR.(lyp/lyp) rat peripheral T cells compared to non-lymphopenic, non-diabetic DR.(+/+) rats. With the exception of Gimap6. the Gimap family genes were not expressed in B cells from spleen and mesenteric lymph node (MLN). Expression of Gimap9 was only detected in hematopoietic cells of non B cell lineage such as macrophage, dendritic or NK cells. Significance: These results suggest that lack of the Gimap5 protein in the DR.(lyp/lyp) congenic rat was associated with impaired expression of the entire family of Gimap genes and may regulate T cell homeostasis in the peripheral lymphoid organs. (C) 2011 Elsevier Inc. All rights reserved.},
  author       = {Moralejo, Daniel H. and Fuller, Jessica and Rutledge, Elizabeth A. and Van Yserloo, Brian and Ettinger, Ruth A. and Jensen, Richard and Osborne, William and Kwitek, Anne and Lernmark, Åke},
  issn         = {1879-0631},
  keyword      = {Gimap,Lymphopenia,Autoimmunity,Type 1 diabetes},
  language     = {eng},
  number       = {19-20},
  pages        = {748--754},
  publisher    = {Elsevier},
  series       = {Life Sciences},
  title        = {BB rat Gimap gene expression in sorted lymphoid T and B cells},
  url          = {http://dx.doi.org/10.1016/j.lfs.2011.08.016},
  volume       = {89},
  year         = {2011},
}