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Establishment and assessments of a new model for the postoperative fatigue syndrome by major small intestinal resection in rats

Zhang, Xiao-Dong; Chen, Bi-Cheng; Dong, Qian-Tong; Andersson, Roland LU ; Pan, Xiao-Dong; Tan, Shan-Jun; Ye, Xing-Zhao; Yao, Jian-Gao and Yu, Zhen (2011) In Scandinavian Journal of Gastroenterology 46(11). p.1302-1309
Abstract
Objective. Postoperative fatigue syndrome (POFS) is a general and main complication after surgery. However, there is no stable and standardized animal model for POFS. The aim of the present study was to establish a rodent model of POFS by small intestinal resection, with POFS evaluated by acknowledged physical and behavioral methods. Material and Methods. Forty-two Sprague-Dawley rats were randomly divided into four groups according to the length of a "middle" small intestinal resection: 0% (sham group; i.e., laparotomy alone), 10%, 40% and 70% groups, with corresponding lengths of small intestinal resections. Following surgery, the general state of health was evaluated. Tail suspension test, open field test and Morris water maze test were... (More)
Objective. Postoperative fatigue syndrome (POFS) is a general and main complication after surgery. However, there is no stable and standardized animal model for POFS. The aim of the present study was to establish a rodent model of POFS by small intestinal resection, with POFS evaluated by acknowledged physical and behavioral methods. Material and Methods. Forty-two Sprague-Dawley rats were randomly divided into four groups according to the length of a "middle" small intestinal resection: 0% (sham group; i.e., laparotomy alone), 10%, 40% and 70% groups, with corresponding lengths of small intestinal resections. Following surgery, the general state of health was evaluated. Tail suspension test, open field test and Morris water maze test were used to evaluate the degree of POFS. Serum albumin, transferrin, prealbumin and fibronectin were measured to assess the nutritional status, and superoxide dismutase (SOD) and malondialdehyde (MDA) were also measured. Results. As compared with the other three groups, the 70% small intestinal resection group showed the worst general state of health, decreased strength of the tail suspension test and decreased score of Morris water maze test (p < 0.05) after operation. All rats in whom the small intestinal resection was done demonstrated a certain degree of malnutrition and behavior of depression, and the 70% resection group had the lowest levels of transferrin, prealbumin and fibronectin as compared with the other groups (p < 0.05), as well as decreased SOD and increased MDA in serum (p < 0.05). Conclusions. Resection of 70% of the small intestine resulted in typical characteristics of POFS. As this procedure is simple, stable and easily reproducible, it may serve as a model for research on POFS. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Animal model, postoperative fatigue syndrome, small intestinal resection
in
Scandinavian Journal of Gastroenterology
volume
46
issue
11
pages
1302 - 1309
publisher
Taylor & Francis
external identifiers
  • wos:000295892100004
  • scopus:80054086146
ISSN
1502-7708
DOI
10.3109/00365521.2011.610001
language
English
LU publication?
yes
id
120ea73b-1b9c-412a-9f54-fd84172f82f1 (old id 2211635)
date added to LUP
2011-12-01 08:36:28
date last changed
2017-01-01 05:47:08
@article{120ea73b-1b9c-412a-9f54-fd84172f82f1,
  abstract     = {Objective. Postoperative fatigue syndrome (POFS) is a general and main complication after surgery. However, there is no stable and standardized animal model for POFS. The aim of the present study was to establish a rodent model of POFS by small intestinal resection, with POFS evaluated by acknowledged physical and behavioral methods. Material and Methods. Forty-two Sprague-Dawley rats were randomly divided into four groups according to the length of a "middle" small intestinal resection: 0% (sham group; i.e., laparotomy alone), 10%, 40% and 70% groups, with corresponding lengths of small intestinal resections. Following surgery, the general state of health was evaluated. Tail suspension test, open field test and Morris water maze test were used to evaluate the degree of POFS. Serum albumin, transferrin, prealbumin and fibronectin were measured to assess the nutritional status, and superoxide dismutase (SOD) and malondialdehyde (MDA) were also measured. Results. As compared with the other three groups, the 70% small intestinal resection group showed the worst general state of health, decreased strength of the tail suspension test and decreased score of Morris water maze test (p &lt; 0.05) after operation. All rats in whom the small intestinal resection was done demonstrated a certain degree of malnutrition and behavior of depression, and the 70% resection group had the lowest levels of transferrin, prealbumin and fibronectin as compared with the other groups (p &lt; 0.05), as well as decreased SOD and increased MDA in serum (p &lt; 0.05). Conclusions. Resection of 70% of the small intestine resulted in typical characteristics of POFS. As this procedure is simple, stable and easily reproducible, it may serve as a model for research on POFS.},
  author       = {Zhang, Xiao-Dong and Chen, Bi-Cheng and Dong, Qian-Tong and Andersson, Roland and Pan, Xiao-Dong and Tan, Shan-Jun and Ye, Xing-Zhao and Yao, Jian-Gao and Yu, Zhen},
  issn         = {1502-7708},
  keyword      = {Animal model,postoperative fatigue syndrome,small intestinal resection},
  language     = {eng},
  number       = {11},
  pages        = {1302--1309},
  publisher    = {Taylor & Francis},
  series       = {Scandinavian Journal of Gastroenterology},
  title        = {Establishment and assessments of a new model for the postoperative fatigue syndrome by major small intestinal resection in rats},
  url          = {http://dx.doi.org/10.3109/00365521.2011.610001},
  volume       = {46},
  year         = {2011},
}