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Serum metabolite signature predicts the acute onset of diabetes in spontaneously diabetic congenic BB rats

Åkesson, Lina LU ; Trygg, Johan; Fuller, Jessica LU ; Madsen, Rasmus; Gabrielsson, Jon; Bruce, Stephen; Stenlund, Hans; Tupling, Terry; Pefley, Ranae and Lundstedt, Torbjorn, et al. (2011) In Metabolomics 7(4). p.593-603
Abstract
The clinical presentation of type 1 diabetes is preceded by a prodrome of beta cell autoimmunity. We probed the short period of subtle metabolic abnormalities, which precede the acute onset of diabetes in the spontaneously diabetic BB rat, by analyzing the serum metabolite profile detected with combined gas chromatography/mass spectrometry (GC/MS) and liquid chromatography/mass spectrometry (LC/MS). We found that the metabolite pattern prior to diabetes included 17 metabolites, which differed between individual diabetes prone (DP) BB rats and their age and sex matched diabetes resistant (DR) littermates. As the metabolite signature at the 40 days of age baseline failed to distinguish DP from DR, there was a brief 10-day period after which... (More)
The clinical presentation of type 1 diabetes is preceded by a prodrome of beta cell autoimmunity. We probed the short period of subtle metabolic abnormalities, which precede the acute onset of diabetes in the spontaneously diabetic BB rat, by analyzing the serum metabolite profile detected with combined gas chromatography/mass spectrometry (GC/MS) and liquid chromatography/mass spectrometry (LC/MS). We found that the metabolite pattern prior to diabetes included 17 metabolites, which differed between individual diabetes prone (DP) BB rats and their age and sex matched diabetes resistant (DR) littermates. As the metabolite signature at the 40 days of age baseline failed to distinguish DP from DR, there was a brief 10-day period after which the diabetes prediction pattern was observed, that includes fatty acids (e.g. oleamide), phospholipids (e.g. phosphocholines) and amino acids (e.g. isoleucine). It is concluded that distinct changes in the serum metabolite pattern predict type 1 diabetes and precede the appearance of insulitis in spontaneously diabetic BB DP rats. This observation should prove useful to dissect mechanisms of type 1 diabetes. (Less)
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Contribution to journal
publication status
published
subject
keywords
Type 1 diabetes, Metabolomics, OPLS-DA, Dynamic modeling
in
Metabolomics
volume
7
issue
4
pages
593 - 603
publisher
Springer
external identifiers
  • wos:000295991900013
  • scopus:80054042661
ISSN
1573-3882
DOI
10.1007/s11306-011-0278-3
language
English
LU publication?
yes
id
a4c69108-d487-479f-88b2-e246555830f9 (old id 2211693)
date added to LUP
2011-12-01 08:36:39
date last changed
2017-02-26 03:04:53
@article{a4c69108-d487-479f-88b2-e246555830f9,
  abstract     = {The clinical presentation of type 1 diabetes is preceded by a prodrome of beta cell autoimmunity. We probed the short period of subtle metabolic abnormalities, which precede the acute onset of diabetes in the spontaneously diabetic BB rat, by analyzing the serum metabolite profile detected with combined gas chromatography/mass spectrometry (GC/MS) and liquid chromatography/mass spectrometry (LC/MS). We found that the metabolite pattern prior to diabetes included 17 metabolites, which differed between individual diabetes prone (DP) BB rats and their age and sex matched diabetes resistant (DR) littermates. As the metabolite signature at the 40 days of age baseline failed to distinguish DP from DR, there was a brief 10-day period after which the diabetes prediction pattern was observed, that includes fatty acids (e.g. oleamide), phospholipids (e.g. phosphocholines) and amino acids (e.g. isoleucine). It is concluded that distinct changes in the serum metabolite pattern predict type 1 diabetes and precede the appearance of insulitis in spontaneously diabetic BB DP rats. This observation should prove useful to dissect mechanisms of type 1 diabetes.},
  author       = {Åkesson, Lina and Trygg, Johan and Fuller, Jessica and Madsen, Rasmus and Gabrielsson, Jon and Bruce, Stephen and Stenlund, Hans and Tupling, Terry and Pefley, Ranae and Lundstedt, Torbjorn and Lernmark, Åke and Moritz, Thomas},
  issn         = {1573-3882},
  keyword      = {Type 1 diabetes,Metabolomics,OPLS-DA,Dynamic modeling},
  language     = {eng},
  number       = {4},
  pages        = {593--603},
  publisher    = {Springer},
  series       = {Metabolomics},
  title        = {Serum metabolite signature predicts the acute onset of diabetes in spontaneously diabetic congenic BB rats},
  url          = {http://dx.doi.org/10.1007/s11306-011-0278-3},
  volume       = {7},
  year         = {2011},
}