Advanced

Dermal uptake study with 4,4'-diphenylmethane diisocyanate led to active sensitization

Hamada, Haneen LU ; Isaksson, Marléne LU ; Bruze, Magnus LU ; Engfeldt, Malin LU ; Liljelind, Ingrid; Axelsson, Sara; Jönsson, Bo A LU ; Tinnerberg, Håkan LU and Zimerson, Erik LU (2012) In Contact Dermatitis 66(2). p.101-105
Abstract
BACKGROUND:
To investigate the dermal uptake of 4,4'-diphenylmethane diisocyanate (4,4'-MDI), a study was performed in which 2 female volunteers were exposed to 10 and 25 mg, respectively, of 4,4'-MDI by applying 2.0% 4,4'-MDI in petrolatum over areas where the surface concentration corresponded to 800 µg/cm(2) . Ten days later, they developed eczematous dermatitis at the area of application.
OBJECTIVES:
To investigate whether the dermal application caused active sensitization to 4,4'-MDI.
METHODS:
Chemical analysis of the 4,4'-MDI preparation used in the application and the amount of 4,4'-MDI not absorbed by the skin was performed with liquid chromatography-mass spectrometry. The volunteers were tested with serial... (More)
BACKGROUND:
To investigate the dermal uptake of 4,4'-diphenylmethane diisocyanate (4,4'-MDI), a study was performed in which 2 female volunteers were exposed to 10 and 25 mg, respectively, of 4,4'-MDI by applying 2.0% 4,4'-MDI in petrolatum over areas where the surface concentration corresponded to 800 µg/cm(2) . Ten days later, they developed eczematous dermatitis at the area of application.
OBJECTIVES:
To investigate whether the dermal application caused active sensitization to 4,4'-MDI.
METHODS:
Chemical analysis of the 4,4'-MDI preparation used in the application and the amount of 4,4'-MDI not absorbed by the skin was performed with liquid chromatography-mass spectrometry. The volunteers were tested with serial dilutions of 4,4'-MDI and the potentially cross-reacting substances 4,4'-diaminodiphenylmethane (4,4'-MDA), p-phenylenediamine (PPD), and dicyclohexylmethane-4,4'-diisocyanate (DMDI).
RESULTS:
Patch test results suggested that the volunteers were actively sensitized to 4,4'-MDI following the dermal uptake study, as they reacted positively to 4,4'-MDA, a marker for 4,4'-MDI allergy. No positive reactions were seen to PPD or DMDI. Chemical investigation confirmed that the correct concentration had been used for the dermal uptake study, and showed that about 70% of the applied 4,4'-MDI was not absorbed.
CONCLUSIONS:
A dermal uptake study with 4,4'-MDI in 2.0% pet. with an occlusion time of 8 hr induced active sensitization to 4,4'-MDI and subsequently to 4,4'-MDA. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
4, 4 '-MDI, 4 '-diaminodiphenylmethane, dicyclohexylmethane-4, '-diisocyanate, DMDI, MDA, patch test, polyurethane, PPD, p-phenylenediamine
in
Contact Dermatitis
volume
66
issue
2
pages
101 - 105
publisher
Federation of European Neuroscience Societies and Blackwell Publishing Ltd
external identifiers
  • wos:000298980100007
  • pmid:22092061
  • scopus:84855674644
ISSN
0105-1873
DOI
10.1111/j.1600-0536.2011.01995.xhttp://dx.doi.org/10.1111/j.1600-0536.2011.01995.x
language
English
LU publication?
yes
id
5d20d060-889d-4407-819c-ce3cfcdf9a86 (old id 2220702)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/22092061?dopt=Abstract
date added to LUP
2011-12-02 21:58:52
date last changed
2017-08-07 10:28:25
@article{5d20d060-889d-4407-819c-ce3cfcdf9a86,
  abstract     = {BACKGROUND:<br/>To investigate the dermal uptake of 4,4'-diphenylmethane diisocyanate (4,4'-MDI), a study was performed in which 2 female volunteers were exposed to 10 and 25 mg, respectively, of 4,4'-MDI by applying 2.0% 4,4'-MDI in petrolatum over areas where the surface concentration corresponded to 800 µg/cm(2) . Ten days later, they developed eczematous dermatitis at the area of application.<br/>OBJECTIVES:<br/>To investigate whether the dermal application caused active sensitization to 4,4'-MDI.<br/>METHODS:<br/>Chemical analysis of the 4,4'-MDI preparation used in the application and the amount of 4,4'-MDI not absorbed by the skin was performed with liquid chromatography-mass spectrometry. The volunteers were tested with serial dilutions of 4,4'-MDI and the potentially cross-reacting substances 4,4'-diaminodiphenylmethane (4,4'-MDA), p-phenylenediamine (PPD), and dicyclohexylmethane-4,4'-diisocyanate (DMDI).<br/>RESULTS:<br/>Patch test results suggested that the volunteers were actively sensitized to 4,4'-MDI following the dermal uptake study, as they reacted positively to 4,4'-MDA, a marker for 4,4'-MDI allergy. No positive reactions were seen to PPD or DMDI. Chemical investigation confirmed that the correct concentration had been used for the dermal uptake study, and showed that about 70% of the applied 4,4'-MDI was not absorbed.<br/>CONCLUSIONS:<br/>A dermal uptake study with 4,4'-MDI in 2.0% pet. with an occlusion time of 8 hr induced active sensitization to 4,4'-MDI and subsequently to 4,4'-MDA.},
  author       = {Hamada, Haneen and Isaksson, Marléne and Bruze, Magnus and Engfeldt, Malin and Liljelind, Ingrid and Axelsson, Sara and Jönsson, Bo A and Tinnerberg, Håkan and Zimerson, Erik},
  issn         = {0105-1873},
  keyword      = {4,4 '-MDI,4 '-diaminodiphenylmethane,dicyclohexylmethane-4,'-diisocyanate,DMDI,MDA,patch test,polyurethane,PPD,p-phenylenediamine},
  language     = {eng},
  number       = {2},
  pages        = {101--105},
  publisher    = {Federation of European Neuroscience Societies and Blackwell Publishing Ltd},
  series       = {Contact Dermatitis},
  title        = {Dermal uptake study with 4,4'-diphenylmethane diisocyanate led to active sensitization},
  url          = {http://dx.doi.org/10.1111/j.1600-0536.2011.01995.xhttp://dx.doi.org/10.1111/j.1600-0536.2011.01995.x},
  volume       = {66},
  year         = {2012},
}