International collaborative proficiency study of Human Papillomavirus type 16 serology.
(2012) In Vaccine 30. p.294-299- Abstract
- We performed an international proficiency study of Human Papillomavirus (HPV) type 16 serology. A common methodology for serology based on virus-like particle (VLP) ELISA was used by 10 laboratories in 6 continents. The laboratories used the same VLP reference reagent, which was selected as the most stable, sensitive and specific VLP preparation out of VLPs donated from 5 different sources. A blinded proficiency panel consisting of 52 serum samples from women with PCR-verified HPV 16-infection, 11 control serum samples from virginal women and the WHO HPV 16 International Standard (IS) serum were distributed. The mean plus 3 standard deviations of the negative control serum samples was the most generally useful "cut-off" criterion for... (More)
- We performed an international proficiency study of Human Papillomavirus (HPV) type 16 serology. A common methodology for serology based on virus-like particle (VLP) ELISA was used by 10 laboratories in 6 continents. The laboratories used the same VLP reference reagent, which was selected as the most stable, sensitive and specific VLP preparation out of VLPs donated from 5 different sources. A blinded proficiency panel consisting of 52 serum samples from women with PCR-verified HPV 16-infection, 11 control serum samples from virginal women and the WHO HPV 16 International Standard (IS) serum were distributed. The mean plus 3 standard deviations of the negative control serum samples was the most generally useful "cut-off" criterion for distinguishing positive and negative samples. Using sensitivity of at least 50% and a specificity of 100% as proficiency criteria, 6/10 laboratories were proficient. In conclusion, an international Standard Operating Procedure for HPV serology, an international reporting system in International Units (IU) and a common "cut-off" criterion have been evaluated in an international HPV serology proficiency study. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2220842
- author
- Eklund, Carina LU ; Unger, Elizabeth R ; Nardelli-Haefliger, Denise ; Zhou, Tiequn and Dillner, Joakim LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Vaccine
- volume
- 30
- pages
- 294 - 299
- publisher
- Elsevier
- external identifiers
-
- wos:000299971800028
- pmid:22079074
- scopus:84355162812
- pmid:22079074
- ISSN
- 1873-2518
- DOI
- 10.1016/j.vaccine.2011.10.096
- language
- English
- LU publication?
- yes
- id
- e020a2a8-b13e-4ce0-b2fc-4bee43645a45 (old id 2220842)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/22079074?dopt=Abstract
- date added to LUP
- 2016-04-04 08:43:53
- date last changed
- 2022-01-29 03:53:44
@article{e020a2a8-b13e-4ce0-b2fc-4bee43645a45, abstract = {{We performed an international proficiency study of Human Papillomavirus (HPV) type 16 serology. A common methodology for serology based on virus-like particle (VLP) ELISA was used by 10 laboratories in 6 continents. The laboratories used the same VLP reference reagent, which was selected as the most stable, sensitive and specific VLP preparation out of VLPs donated from 5 different sources. A blinded proficiency panel consisting of 52 serum samples from women with PCR-verified HPV 16-infection, 11 control serum samples from virginal women and the WHO HPV 16 International Standard (IS) serum were distributed. The mean plus 3 standard deviations of the negative control serum samples was the most generally useful "cut-off" criterion for distinguishing positive and negative samples. Using sensitivity of at least 50% and a specificity of 100% as proficiency criteria, 6/10 laboratories were proficient. In conclusion, an international Standard Operating Procedure for HPV serology, an international reporting system in International Units (IU) and a common "cut-off" criterion have been evaluated in an international HPV serology proficiency study.}}, author = {{Eklund, Carina and Unger, Elizabeth R and Nardelli-Haefliger, Denise and Zhou, Tiequn and Dillner, Joakim}}, issn = {{1873-2518}}, language = {{eng}}, pages = {{294--299}}, publisher = {{Elsevier}}, series = {{Vaccine}}, title = {{International collaborative proficiency study of Human Papillomavirus type 16 serology.}}, url = {{http://dx.doi.org/10.1016/j.vaccine.2011.10.096}}, doi = {{10.1016/j.vaccine.2011.10.096}}, volume = {{30}}, year = {{2012}}, }