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Helicobacter species and gut bacterial DNA in Meckel's diverticulum and the appendix.

Karagin, Peren H ; Stenram, Unne LU ; Wadström, Torkel LU and Ljungh, Åsa LU (2011) In World Journal of Gastroenterology 17(36). p.4104-4108
Abstract
AIM: To analyse the possible association of various Helicobacter species and certain common gut bacteria in patients with Meckel's diverticulum and appendicitis.



METHODS:

A nested-polymerase chain reaction (PCR), specific to 16S rRNA of the Helicobacter genus, was performed on paraffin embedded samples, 50 with acute appendicitis, 50 normal appendixes, and 33 Meckel's diverticulum with gastric heterotopia and/or ulcer. Helicobacter genus positive samples were sequenced for species identification. All samples were also analysed for certain gut bacteria by PCR.



RESULTS:

Helicobacter pullorum DNA was found in one out of 33 cases and Enterobacteria in two cases of Meckel's diverticulum.... (More)
AIM: To analyse the possible association of various Helicobacter species and certain common gut bacteria in patients with Meckel's diverticulum and appendicitis.



METHODS:

A nested-polymerase chain reaction (PCR), specific to 16S rRNA of the Helicobacter genus, was performed on paraffin embedded samples, 50 with acute appendicitis, 50 normal appendixes, and 33 Meckel's diverticulum with gastric heterotopia and/or ulcer. Helicobacter genus positive samples were sequenced for species identification. All samples were also analysed for certain gut bacteria by PCR.



RESULTS:

Helicobacter pullorum DNA was found in one out of 33 cases and Enterobacteria in two cases of Meckel's diverticulum. Helicobacter pylori (H. pylori) was found in three, Enterobacter in 18, and Bacteroides in 19 out of 100 appendix samples by PCR. Enterococcus was not found in any MD or appendix samples. All H. pylori positive cases were from normal appendixes.



CONCLUSION:

Helicobacter is not an etiological agent in the pathogenesis of symptomatic Meckel's diverticulum or in acute appendicitis. (Less)
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
World Journal of Gastroenterology
volume
17
issue
36
pages
4104 - 4108
publisher
WJG Press
external identifiers
  • wos:000296871300007
  • pmid:22039324
  • scopus:80155191167
  • pmid:22039324
ISSN
1007-9327
DOI
10.3748/wjg.v17.i36.4104
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Pathology, (Lund) (013030000), Division of Medical Microbiology (013250400)
id
ef9b9a71-05c3-4f34-afbf-9f249463e927 (old id 2221386)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/22039324?dopt=Abstract
date added to LUP
2016-04-04 08:47:22
date last changed
2021-07-13 03:12:17
@article{ef9b9a71-05c3-4f34-afbf-9f249463e927,
  abstract     = {AIM: To analyse the possible association of various Helicobacter species and certain common gut bacteria in patients with Meckel's diverticulum and appendicitis.<br/><br>
<br/><br>
METHODS:<br/><br>
A nested-polymerase chain reaction (PCR), specific to 16S rRNA of the Helicobacter genus, was performed on paraffin embedded samples, 50 with acute appendicitis, 50 normal appendixes, and 33 Meckel's diverticulum with gastric heterotopia and/or ulcer. Helicobacter genus positive samples were sequenced for species identification. All samples were also analysed for certain gut bacteria by PCR.<br/><br>
<br/><br>
RESULTS:<br/><br>
Helicobacter pullorum DNA was found in one out of 33 cases and Enterobacteria in two cases of Meckel's diverticulum. Helicobacter pylori (H. pylori) was found in three, Enterobacter in 18, and Bacteroides in 19 out of 100 appendix samples by PCR. Enterococcus was not found in any MD or appendix samples. All H. pylori positive cases were from normal appendixes.<br/><br>
<br/><br>
CONCLUSION:<br/><br>
Helicobacter is not an etiological agent in the pathogenesis of symptomatic Meckel's diverticulum or in acute appendicitis.},
  author       = {Karagin, Peren H and Stenram, Unne and Wadström, Torkel and Ljungh, Åsa},
  issn         = {1007-9327},
  language     = {eng},
  number       = {36},
  pages        = {4104--4108},
  publisher    = {WJG Press},
  series       = {World Journal of Gastroenterology},
  title        = {Helicobacter species and gut bacterial DNA in Meckel's diverticulum and the appendix.},
  url          = {http://dx.doi.org/10.3748/wjg.v17.i36.4104},
  doi          = {10.3748/wjg.v17.i36.4104},
  volume       = {17},
  year         = {2011},
}