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Inefficient splicing of segment 7 and 8 mRNAs is an inherent property of influenza virus A/Brevig Mission/1918/1 (H1N1) that causes elevated expression of NS1 protein.

Winquist, Ellenor LU ; Abdurahman, Samir; Tranell, Anna; Lindström, Sofia; Tingsborg, Susanne and Schwartz, Stefan LU (2012) In Virology 422. p.46-58
Abstract
Influenza A virus encodes two segments (7 and 8) that produce mRNAs that can be spliced. We have investigated if naturally occurring sequence polymorphisms in the influenza A virus family affects splicing of these viral mRNAs, as that could potentially alter the NS1/NS2- and/or M1/M2-protein ratios. We compared splicing efficiency of segment 7 and 8 mRNAs of A/Brevig Mission/1918/1 (H1N1) and A/Netherlands/178/95 (H3N2), as well as various H5N1 avian strains. Results revealed that both segment 7 and 8 mRNAs of A/Brevig Mission/1918/1 (H1N1) were inefficiently spliced compared to other influenza virus segment 7 and 8 mRNAs. This resulted in production of higher levels of functional NS1 protein, which could potentially contribute to the... (More)
Influenza A virus encodes two segments (7 and 8) that produce mRNAs that can be spliced. We have investigated if naturally occurring sequence polymorphisms in the influenza A virus family affects splicing of these viral mRNAs, as that could potentially alter the NS1/NS2- and/or M1/M2-protein ratios. We compared splicing efficiency of segment 7 and 8 mRNAs of A/Brevig Mission/1918/1 (H1N1) and A/Netherlands/178/95 (H3N2), as well as various H5N1 avian strains. Results revealed that both segment 7 and 8 mRNAs of A/Brevig Mission/1918/1 (H1N1) were inefficiently spliced compared to other influenza virus segment 7 and 8 mRNAs. This resulted in production of higher levels of functional NS1 protein, which could potentially contribute to the pathogenic properties of the A/Brevig Mission/1918/1 (H1N1). We also show that A/Brevig Mission/1918/1 (H1N1) segment 8 mRNAs responded differently to overexpression of SR proteins than A/Netherlands/178/95 (H3N2). (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Virology
volume
422
pages
46 - 58
publisher
Elsevier
external identifiers
  • WOS:000297902300006
  • PMID:22036312
  • Scopus:82355190150
ISSN
1096-0341
DOI
10.1016/j.virol.2011.10.004
language
English
LU publication?
yes
id
d65fbd37-d0d3-4789-8095-ceea2f13c4db (old id 2221413)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/22036312?dopt=Abstract
date added to LUP
2011-12-01 14:52:17
date last changed
2017-01-01 07:46:53
@article{d65fbd37-d0d3-4789-8095-ceea2f13c4db,
  abstract     = {Influenza A virus encodes two segments (7 and 8) that produce mRNAs that can be spliced. We have investigated if naturally occurring sequence polymorphisms in the influenza A virus family affects splicing of these viral mRNAs, as that could potentially alter the NS1/NS2- and/or M1/M2-protein ratios. We compared splicing efficiency of segment 7 and 8 mRNAs of A/Brevig Mission/1918/1 (H1N1) and A/Netherlands/178/95 (H3N2), as well as various H5N1 avian strains. Results revealed that both segment 7 and 8 mRNAs of A/Brevig Mission/1918/1 (H1N1) were inefficiently spliced compared to other influenza virus segment 7 and 8 mRNAs. This resulted in production of higher levels of functional NS1 protein, which could potentially contribute to the pathogenic properties of the A/Brevig Mission/1918/1 (H1N1). We also show that A/Brevig Mission/1918/1 (H1N1) segment 8 mRNAs responded differently to overexpression of SR proteins than A/Netherlands/178/95 (H3N2).},
  author       = {Winquist, Ellenor and Abdurahman, Samir and Tranell, Anna and Lindström, Sofia and Tingsborg, Susanne and Schwartz, Stefan},
  issn         = {1096-0341},
  language     = {eng},
  pages        = {46--58},
  publisher    = {Elsevier},
  series       = {Virology},
  title        = {Inefficient splicing of segment 7 and 8 mRNAs is an inherent property of influenza virus A/Brevig Mission/1918/1 (H1N1) that causes elevated expression of NS1 protein.},
  url          = {http://dx.doi.org/10.1016/j.virol.2011.10.004},
  volume       = {422},
  year         = {2012},
}