Advanced

Growth and assimilation of NH4+ and NO3- by Paxillus involutus in association with Betula pendula and Picea abies as affected by substrate pH

Ek, H.,; Andersson, S.,; Arnebrant, Kristina LU and Söderström, Bengt LU (1994) In New Phytologist 128(4). p.629-637
Abstract
The influence of pH on the growth and assimilation of N-15-labelled ammonium and nitrate was studied in intact ectomycorrhizal systems consisting of Betula pendula Roth and Picea abies (L.) Karst. colonized with a common mycelium of Paxillus involutus (Batsch) Fr. The plants were grown together in Plexiglass observation chambers containing non-sterile peat with three different pH values, 4.0, 5.1 and 6.1. The mycorrhizal mycelium was allowed to grow over a barrier into an area of peat from which plant roots were excluded. Labelled NH4NO3 was supplied, either as (NH4NO3)-N-15 or as (NH4NO3)-N-15, exclusively to the fungal mycelium. Shoots and roots were analyzed for N-15 in total nitrogen while the mycelium was analyzed for N-15 in NH4+,... (More)
The influence of pH on the growth and assimilation of N-15-labelled ammonium and nitrate was studied in intact ectomycorrhizal systems consisting of Betula pendula Roth and Picea abies (L.) Karst. colonized with a common mycelium of Paxillus involutus (Batsch) Fr. The plants were grown together in Plexiglass observation chambers containing non-sterile peat with three different pH values, 4.0, 5.1 and 6.1. The mycorrhizal mycelium was allowed to grow over a barrier into an area of peat from which plant roots were excluded. Labelled NH4NO3 was supplied, either as (NH4NO3)-N-15 or as (NH4NO3)-N-15, exclusively to the fungal mycelium. Shoots and roots were analyzed for N-15 in total nitrogen while the mycelium was analyzed for N-15 in NH4+, NO3- and free amino acids. The N-15 labelling pattern indicated that ammonium was immediately assimilated into amino acids, primarily glutamine, by the fungal mycelium at the uptake site. The amino acids were then translocated to the mycorrhizal roots. In contrast, nitrate-N was not assimilated in the mycelium but rather transferred to the mycorrhizal roots as nitrate. Mycelial uptake and transfer of N to the spruce and birch seedlings were significantly higher for NH4-N than for NO3-N. No firm conclusions about pH effects on the preferential uptake of ammonium and nitrate could be drawn. However, pH had a pronounced effect on the mycelial growth of P. involutus which was hampered severely at pH 6.1 and to a lesser extent at pH 5.1. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Mycorrhizas, ammonium, nitrate, amino acids, 16N
in
New Phytologist
volume
128
issue
4
pages
629 - 637
publisher
Wiley-Blackwell
external identifiers
  • scopus:0028253770
ISSN
1469-8137
DOI
10.1111/j.1469-8137.1994.tb04027.x
language
English
LU publication?
yes
id
8fa30721-c828-4d24-b744-64400f6356a5 (old id 2226316)
alternative location
http://www.jstor.org/stable/2558370
date added to LUP
2012-01-11 15:56:41
date last changed
2017-07-30 03:51:39
@article{8fa30721-c828-4d24-b744-64400f6356a5,
  abstract     = {The influence of pH on the growth and assimilation of N-15-labelled ammonium and nitrate was studied in intact ectomycorrhizal systems consisting of Betula pendula Roth and Picea abies (L.) Karst. colonized with a common mycelium of Paxillus involutus (Batsch) Fr. The plants were grown together in Plexiglass observation chambers containing non-sterile peat with three different pH values, 4.0, 5.1 and 6.1. The mycorrhizal mycelium was allowed to grow over a barrier into an area of peat from which plant roots were excluded. Labelled NH4NO3 was supplied, either as (NH4NO3)-N-15 or as (NH4NO3)-N-15, exclusively to the fungal mycelium. Shoots and roots were analyzed for N-15 in total nitrogen while the mycelium was analyzed for N-15 in NH4+, NO3- and free amino acids. The N-15 labelling pattern indicated that ammonium was immediately assimilated into amino acids, primarily glutamine, by the fungal mycelium at the uptake site. The amino acids were then translocated to the mycorrhizal roots. In contrast, nitrate-N was not assimilated in the mycelium but rather transferred to the mycorrhizal roots as nitrate. Mycelial uptake and transfer of N to the spruce and birch seedlings were significantly higher for NH4-N than for NO3-N. No firm conclusions about pH effects on the preferential uptake of ammonium and nitrate could be drawn. However, pH had a pronounced effect on the mycelial growth of P. involutus which was hampered severely at pH 6.1 and to a lesser extent at pH 5.1.},
  author       = {Ek, H., and Andersson, S., and Arnebrant, Kristina and Söderström, Bengt},
  issn         = {1469-8137},
  keyword      = {Mycorrhizas,ammonium,nitrate,amino acids,16N},
  language     = {eng},
  number       = {4},
  pages        = {629--637},
  publisher    = {Wiley-Blackwell},
  series       = {New Phytologist},
  title        = {Growth and assimilation of NH4+ and NO3- by Paxillus involutus in association with Betula pendula and Picea abies as affected by substrate pH},
  url          = {http://dx.doi.org/10.1111/j.1469-8137.1994.tb04027.x},
  volume       = {128},
  year         = {1994},
}