Effects of liming on ectomycorrhizal fungi infecting Pinus sylvestris L .1. Mycorrhizal infection in limed humus in the laboratory and isolation of fungi from mycorrhizal roots
(1990) In New Phytologist 115(4). p.675-682- Abstract
- Pinus sylvestris L. seedlings were grown in the laboratory in a forest humus which had been treated with different levels of CaO to give a soil pH gradient from 4 to 7. After 12 and 15 wk the resulting ectomycorrhizal infection was classified according to macroscopical morphology. The uniformity of the infection types distinguished was evaluated by isolating the fungi from the root tips and infecting seedlings with the isolates obtained. The isolation method was improved by using a medium containing benomyl and chlortetracyclin and by isolating from very small particles. For some mycorrhizal types the fungus was successfully isolated from 80% of the plated root particles. The number of root tips that became mycorrhizal increased from 70%... (More)
- Pinus sylvestris L. seedlings were grown in the laboratory in a forest humus which had been treated with different levels of CaO to give a soil pH gradient from 4 to 7. After 12 and 15 wk the resulting ectomycorrhizal infection was classified according to macroscopical morphology. The uniformity of the infection types distinguished was evaluated by isolating the fungi from the root tips and infecting seedlings with the isolates obtained. The isolation method was improved by using a medium containing benomyl and chlortetracyclin and by isolating from very small particles. For some mycorrhizal types the fungus was successfully isolated from 80% of the plated root particles. The number of root tips that became mycorrhizal increased from 70% at pH 4 to nearly 100% around pH 5. Thereafter it decreased linearly to reach a minimum of slightly less than 40% at pH 7.5. Five different mycorrhizal types could be distinguished. They occurred with different abundance, distribution and pH optima. They were present throughout the entire pH range, except for a yellow type, identified as Piloderma croceum Erikss. & Hjortst., which was not found at values higher than 6.2. There was no great differences between infections on plants harvested after 12 and 15 wk. Seedling growth was constant to pH 5 then it increased up to about pH 7, at higher pH values growth declined. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2226363
- author
- Erland, Susanne LU and Söderström, Bengt LU
- organization
- publishing date
- 1990
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Ectomycorrhiza, liming, fungal isolation, Pinus sylvestris, humus
- in
- New Phytologist
- volume
- 115
- issue
- 4
- pages
- 675 - 682
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:0025693586
- ISSN
- 1469-8137
- language
- English
- LU publication?
- yes
- id
- ad7a945e-2873-424f-9bcc-47bc830bea43 (old id 2226363)
- alternative location
- http://www.jstor.org/stable/2556809
- date added to LUP
- 2016-04-01 11:59:07
- date last changed
- 2024-01-08 03:52:02
@article{ad7a945e-2873-424f-9bcc-47bc830bea43,
abstract = {{Pinus sylvestris L. seedlings were grown in the laboratory in a forest humus which had been treated with different levels of CaO to give a soil pH gradient from 4 to 7. After 12 and 15 wk the resulting ectomycorrhizal infection was classified according to macroscopical morphology. The uniformity of the infection types distinguished was evaluated by isolating the fungi from the root tips and infecting seedlings with the isolates obtained. The isolation method was improved by using a medium containing benomyl and chlortetracyclin and by isolating from very small particles. For some mycorrhizal types the fungus was successfully isolated from 80% of the plated root particles. The number of root tips that became mycorrhizal increased from 70% at pH 4 to nearly 100% around pH 5. Thereafter it decreased linearly to reach a minimum of slightly less than 40% at pH 7.5. Five different mycorrhizal types could be distinguished. They occurred with different abundance, distribution and pH optima. They were present throughout the entire pH range, except for a yellow type, identified as Piloderma croceum Erikss. & Hjortst., which was not found at values higher than 6.2. There was no great differences between infections on plants harvested after 12 and 15 wk. Seedling growth was constant to pH 5 then it increased up to about pH 7, at higher pH values growth declined.}},
author = {{Erland, Susanne and Söderström, Bengt}},
issn = {{1469-8137}},
keywords = {{Ectomycorrhiza; liming; fungal isolation; Pinus sylvestris; humus}},
language = {{eng}},
number = {{4}},
pages = {{675--682}},
publisher = {{Wiley-Blackwell}},
series = {{New Phytologist}},
title = {{Effects of liming on ectomycorrhizal fungi infecting Pinus sylvestris L .1. Mycorrhizal infection in limed humus in the laboratory and isolation of fungi from mycorrhizal roots}},
url = {{http://www.jstor.org/stable/2556809}},
volume = {{115}},
year = {{1990}},
}