Adenovirus-mediated silencing of Synaptotagmin 9 inhibits Ca2+-dependent insulin secretion in islets
(2005) In FEBS Letters 579(23). p.5241-5246- Abstract
- Synaptotagmins (Syts) are involved in Ca2+-dependent insulin release. However, which Syt isoform is functional in primary beta-cells remains unknown. We demonstrate by electron microscopy of pancreatic islets, the association of Syt 9 with insulin granules. Silencing of Syt 9 by RNA interference adenovirus in islet cells had no effect on the expression of Syt 5, Syt 7 and Syt 3 isoforms. The latter was localized at the plasma membrane of pancreatic polypeptide cells. Insulin release in response to glucose or tolbutamide was strongly inhibited in Syt 9 deficient islets, whereas exocytosis potentiated by raising cAMP levels, was unaltered. Thus, Syt 9 may act as Ca2+ sensor for beta-cell secretion.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/223129
- author
- Iezzi, M ; Eliasson, Lena LU ; Fukuda, M and Wollheim, C B
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- insulin exocytosis, pancreatic islets, electron, RNA, microscopy, interference, beta-cell
- in
- FEBS Letters
- volume
- 579
- issue
- 23
- pages
- 5241 - 5246
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:16165130
- wos:000232194300024
- scopus:24944458103
- pmid:16165130
- ISSN
- 1873-3468
- DOI
- 10.1016/j.febslet.2005.08.047
- language
- English
- LU publication?
- yes
- id
- 186553df-d08f-402a-a699-0ec381315ef9 (old id 223129)
- date added to LUP
- 2016-04-01 16:20:17
- date last changed
- 2022-01-28 19:01:19
@article{186553df-d08f-402a-a699-0ec381315ef9, abstract = {{Synaptotagmins (Syts) are involved in Ca2+-dependent insulin release. However, which Syt isoform is functional in primary beta-cells remains unknown. We demonstrate by electron microscopy of pancreatic islets, the association of Syt 9 with insulin granules. Silencing of Syt 9 by RNA interference adenovirus in islet cells had no effect on the expression of Syt 5, Syt 7 and Syt 3 isoforms. The latter was localized at the plasma membrane of pancreatic polypeptide cells. Insulin release in response to glucose or tolbutamide was strongly inhibited in Syt 9 deficient islets, whereas exocytosis potentiated by raising cAMP levels, was unaltered. Thus, Syt 9 may act as Ca2+ sensor for beta-cell secretion.}}, author = {{Iezzi, M and Eliasson, Lena and Fukuda, M and Wollheim, C B}}, issn = {{1873-3468}}, keywords = {{insulin exocytosis; pancreatic islets; electron; RNA; microscopy; interference; beta-cell}}, language = {{eng}}, number = {{23}}, pages = {{5241--5246}}, publisher = {{Wiley-Blackwell}}, series = {{FEBS Letters}}, title = {{Adenovirus-mediated silencing of Synaptotagmin 9 inhibits Ca2+-dependent insulin secretion in islets}}, url = {{http://dx.doi.org/10.1016/j.febslet.2005.08.047}}, doi = {{10.1016/j.febslet.2005.08.047}}, volume = {{579}}, year = {{2005}}, }