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Dynamic distribution of endoplasmic reticulum in hippocampal neuron dendritic spines

Toresson, Håkan LU and Grant, S G N (2005) In European Journal of Neuroscience 22(7). p.1793-1798
Abstract
The role of the endoplasmic reticulum (ER) localized in dendritic spines has become a subject of intense interest because of its potential functions in local protein synthesis and signal transduction. Although it is recognized from electron microscopic studies that not all spines contain ER, little is know of its dynamic regulation or turnover. Here, we report a surprising degree of turnover of ER within spines. Using confocal microscopy imaging we observed continuity of spine-ER with dendritic ER in hippocampal primary neurons. Over 24 h, less than 50% of spine ER was stable. Despite this high degree of turn over, we identified a significant subset of spines that maintained ER for at least 4 days. These results indicate that within a... (More)
The role of the endoplasmic reticulum (ER) localized in dendritic spines has become a subject of intense interest because of its potential functions in local protein synthesis and signal transduction. Although it is recognized from electron microscopic studies that not all spines contain ER, little is know of its dynamic regulation or turnover. Here, we report a surprising degree of turnover of ER within spines. Using confocal microscopy imaging we observed continuity of spine-ER with dendritic ER in hippocampal primary neurons. Over 24 h, less than 50% of spine ER was stable. Despite this high degree of turn over, we identified a significant subset of spines that maintained ER for at least 4 days. These results indicate that within a single neuron, the organelle composition of a spine is unexpectedly dynamic and may explain aspects of the spine-to-spine variation in calcium spike magnitude and localized protein synthesis and trafficking. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
imaging, light microscopy, hippocampus, endoplasmic reticulum, dendritic spine, primary culture
in
European Journal of Neuroscience
volume
22
issue
7
pages
1793 - 1798
publisher
Wiley-Blackwell
external identifiers
  • wos:000232234700025
  • pmid:16197520
  • scopus:26944455925
ISSN
1460-9568
DOI
10.1111/j.1460-9568.2005.04342.x
language
English
LU publication?
yes
id
5f099b93-0606-4dde-bfcf-ef292690d436 (old id 223157)
date added to LUP
2007-08-10 09:32:43
date last changed
2017-04-23 03:37:06
@article{5f099b93-0606-4dde-bfcf-ef292690d436,
  abstract     = {The role of the endoplasmic reticulum (ER) localized in dendritic spines has become a subject of intense interest because of its potential functions in local protein synthesis and signal transduction. Although it is recognized from electron microscopic studies that not all spines contain ER, little is know of its dynamic regulation or turnover. Here, we report a surprising degree of turnover of ER within spines. Using confocal microscopy imaging we observed continuity of spine-ER with dendritic ER in hippocampal primary neurons. Over 24 h, less than 50% of spine ER was stable. Despite this high degree of turn over, we identified a significant subset of spines that maintained ER for at least 4 days. These results indicate that within a single neuron, the organelle composition of a spine is unexpectedly dynamic and may explain aspects of the spine-to-spine variation in calcium spike magnitude and localized protein synthesis and trafficking.},
  author       = {Toresson, Håkan and Grant, S G N},
  issn         = {1460-9568},
  keyword      = {imaging,light microscopy,hippocampus,endoplasmic reticulum,dendritic spine,primary culture},
  language     = {eng},
  number       = {7},
  pages        = {1793--1798},
  publisher    = {Wiley-Blackwell},
  series       = {European Journal of Neuroscience},
  title        = {Dynamic distribution of endoplasmic reticulum in hippocampal neuron dendritic spines},
  url          = {http://dx.doi.org/10.1111/j.1460-9568.2005.04342.x},
  volume       = {22},
  year         = {2005},
}