Dynamic distribution of endoplasmic reticulum in hippocampal neuron dendritic spines
(2005) In European Journal of Neuroscience 22(7). p.1793-1798- Abstract
- The role of the endoplasmic reticulum (ER) localized in dendritic spines has become a subject of intense interest because of its potential functions in local protein synthesis and signal transduction. Although it is recognized from electron microscopic studies that not all spines contain ER, little is know of its dynamic regulation or turnover. Here, we report a surprising degree of turnover of ER within spines. Using confocal microscopy imaging we observed continuity of spine-ER with dendritic ER in hippocampal primary neurons. Over 24 h, less than 50% of spine ER was stable. Despite this high degree of turn over, we identified a significant subset of spines that maintained ER for at least 4 days. These results indicate that within a... (More)
- The role of the endoplasmic reticulum (ER) localized in dendritic spines has become a subject of intense interest because of its potential functions in local protein synthesis and signal transduction. Although it is recognized from electron microscopic studies that not all spines contain ER, little is know of its dynamic regulation or turnover. Here, we report a surprising degree of turnover of ER within spines. Using confocal microscopy imaging we observed continuity of spine-ER with dendritic ER in hippocampal primary neurons. Over 24 h, less than 50% of spine ER was stable. Despite this high degree of turn over, we identified a significant subset of spines that maintained ER for at least 4 days. These results indicate that within a single neuron, the organelle composition of a spine is unexpectedly dynamic and may explain aspects of the spine-to-spine variation in calcium spike magnitude and localized protein synthesis and trafficking. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/223157
- author
- Toresson, Håkan LU and Grant, S G N
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- imaging, light microscopy, hippocampus, endoplasmic reticulum, dendritic spine, primary culture
- in
- European Journal of Neuroscience
- volume
- 22
- issue
- 7
- pages
- 1793 - 1798
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000232234700025
- pmid:16197520
- scopus:26944455925
- ISSN
- 1460-9568
- DOI
- 10.1111/j.1460-9568.2005.04342.x
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Laboratory for Experimental Brain Research (013041000)
- id
- 5f099b93-0606-4dde-bfcf-ef292690d436 (old id 223157)
- date added to LUP
- 2016-04-01 12:12:26
- date last changed
- 2022-02-11 03:32:13
@article{5f099b93-0606-4dde-bfcf-ef292690d436,
abstract = {{The role of the endoplasmic reticulum (ER) localized in dendritic spines has become a subject of intense interest because of its potential functions in local protein synthesis and signal transduction. Although it is recognized from electron microscopic studies that not all spines contain ER, little is know of its dynamic regulation or turnover. Here, we report a surprising degree of turnover of ER within spines. Using confocal microscopy imaging we observed continuity of spine-ER with dendritic ER in hippocampal primary neurons. Over 24 h, less than 50% of spine ER was stable. Despite this high degree of turn over, we identified a significant subset of spines that maintained ER for at least 4 days. These results indicate that within a single neuron, the organelle composition of a spine is unexpectedly dynamic and may explain aspects of the spine-to-spine variation in calcium spike magnitude and localized protein synthesis and trafficking.}},
author = {{Toresson, Håkan and Grant, S G N}},
issn = {{1460-9568}},
keywords = {{imaging; light microscopy; hippocampus; endoplasmic reticulum; dendritic spine; primary culture}},
language = {{eng}},
number = {{7}},
pages = {{1793--1798}},
publisher = {{Wiley-Blackwell}},
series = {{European Journal of Neuroscience}},
title = {{Dynamic distribution of endoplasmic reticulum in hippocampal neuron dendritic spines}},
url = {{http://dx.doi.org/10.1111/j.1460-9568.2005.04342.x}},
doi = {{10.1111/j.1460-9568.2005.04342.x}},
volume = {{22}},
year = {{2005}},
}