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Glucose receptor deletion and engineering : Impact on xylose sensing and utilization in Saccharomyces cerevisiae

Bolzico, Bruna C. ; Persson, Viktor C. LU orcid ; Comelli, Raul N. and Gorwa-Grauslund, Marie LU (2025) In FEMS Yeast Research 25.
Abstract

Unlike glucose, the sub-optimal xylose utilization in recombinant Saccharomyces cerevisiae strains may stem from an unusual signaling response that is not adapted to detecting xylose as a fermentable substrate. We hypothesize that the membrane receptor Snf3p, known for sensing extracellular low glucose levels, may contribute to xylose recognition. To test this, we explored the effect of SNF3 inactivation and overexpression by measuring the response of the HXT2p-GFP biosensor integrated into S. cerevisiae strains with heterogeneous xylose assimilation and metabolism capacities. We showed that the absence of SNF3 effectively reduced HXT2p induction, while its overexpression improved signaling in the presence of xylose, suggesting the... (More)

Unlike glucose, the sub-optimal xylose utilization in recombinant Saccharomyces cerevisiae strains may stem from an unusual signaling response that is not adapted to detecting xylose as a fermentable substrate. We hypothesize that the membrane receptor Snf3p, known for sensing extracellular low glucose levels, may contribute to xylose recognition. To test this, we explored the effect of SNF3 inactivation and overexpression by measuring the response of the HXT2p-GFP biosensor integrated into S. cerevisiae strains with heterogeneous xylose assimilation and metabolism capacities. We showed that the absence of SNF3 effectively reduced HXT2p induction, while its overexpression improved signaling in the presence of xylose, suggesting the involvement of the receptor in the extracellular detection of this sugar. Although we attempted to engineer a xylose sensing system based on a chimeric receptor, its integration did not lead to considerable improvements in signal activation, indicating the need for further investigation. Finally, we showed that triggering the Snf3p pathway impacted xylose metabolism, with altered receptor levels prompting shifts in both biomass production and metabolite accumulation. Our findings suggest that understanding xylose sensing and its metabolic connection is essential for promoting more efficient xylose utilization in S. cerevisiae, a key step toward optimizing industrial bioprocesses.

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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
GFP-based biosensor, Hxt2p, receptor engineering, saccharomyces cerevisiae, Snf3p, sugar signaling, xylose
in
FEMS Yeast Research
volume
25
article number
foaf040
publisher
Oxford University Press
external identifiers
  • scopus:105013603970
  • pmid:40728911
ISSN
1567-1356
DOI
10.1093/femsyr/foaf040
language
English
LU publication?
yes
id
223c3d46-e74a-47d3-9732-e75b114b27c4
date added to LUP
2025-11-19 11:24:51
date last changed
2025-11-19 11:25:58
@article{223c3d46-e74a-47d3-9732-e75b114b27c4,
  abstract     = {{<p>Unlike glucose, the sub-optimal xylose utilization in recombinant Saccharomyces cerevisiae strains may stem from an unusual signaling response that is not adapted to detecting xylose as a fermentable substrate. We hypothesize that the membrane receptor Snf3p, known for sensing extracellular low glucose levels, may contribute to xylose recognition. To test this, we explored the effect of SNF3 inactivation and overexpression by measuring the response of the HXT2p-GFP biosensor integrated into S. cerevisiae strains with heterogeneous xylose assimilation and metabolism capacities. We showed that the absence of SNF3 effectively reduced HXT2p induction, while its overexpression improved signaling in the presence of xylose, suggesting the involvement of the receptor in the extracellular detection of this sugar. Although we attempted to engineer a xylose sensing system based on a chimeric receptor, its integration did not lead to considerable improvements in signal activation, indicating the need for further investigation. Finally, we showed that triggering the Snf3p pathway impacted xylose metabolism, with altered receptor levels prompting shifts in both biomass production and metabolite accumulation. Our findings suggest that understanding xylose sensing and its metabolic connection is essential for promoting more efficient xylose utilization in S. cerevisiae, a key step toward optimizing industrial bioprocesses.</p>}},
  author       = {{Bolzico, Bruna C. and Persson, Viktor C. and Comelli, Raul N. and Gorwa-Grauslund, Marie}},
  issn         = {{1567-1356}},
  keywords     = {{GFP-based biosensor; Hxt2p; receptor engineering; saccharomyces cerevisiae; Snf3p; sugar signaling; xylose}},
  language     = {{eng}},
  publisher    = {{Oxford University Press}},
  series       = {{FEMS Yeast Research}},
  title        = {{Glucose receptor deletion and engineering : Impact on xylose sensing and utilization in Saccharomyces cerevisiae}},
  url          = {{http://dx.doi.org/10.1093/femsyr/foaf040}},
  doi          = {{10.1093/femsyr/foaf040}},
  volume       = {{25}},
  year         = {{2025}},
}